956 research outputs found
Blood on white picket fences : the American dream in George A. Romero's living dead nightmare.
"George A. Romero has been called the "Father of the Modern Zombie Movie." His 1968 classic, Night of the Living Dead, sparked an entirely new sub-genre of horror cinema. Along with this new medium of fright came a new way to interpret America. The Dead series brings in aspects of the American Dream including racial tensions, home ownership, and consumerism and how survivors must cope with the new menace and each other within an apocalyptic scenario. Each film offers a glimpse into the extra-filmic culture surrounding the films' release allowing the audience to interpret the socio-historical subtext. Romero's films demonstrate the inability for human cooperation to occur within a disaster scenario, and that this spells as the ultimate end for the status quo. As for the living dead, the shambling masses are the mirror of the human society that is on its knees, operating on an instinctual mode of mass consumption. As the films progress, the living dead become self-aware and move to protect their own society they have taken from the survivors. The American Dream, the fallen society followed, only turned humanity into the living dead, and those that kept with the American Dream after the beginning of the contagion were not capable of surviving within such a hostile environment."--Abstract.
In-frame Mutagenesis Of Genes Encoding A Selenium-dependent Molybdenum Hydroxylase And Putative Accessory Proteins In Enterococcus Faecalis
Enterococcus faecalis is a well known nosocomial drug resistant pathogen that is responsible for urinary tract infections, bacteremia, wound infections and endocarditis through the formation of biofilms. It has been shown that 68 genes present within the core genome of E. faecalis are upregulated in biofilm formation. One of those 68 genes is a putative seleniumdependent molybdenum hydroxylase (SDMH). Adjacent to this gene are a series of open reading frames that have been postulated to play a role in the maturation of a labile selenium cofactor. The biosynthesis of this labile cofactor has yet to be studied at either the genetic or biochemical level. The addition of selenium to growth medium caused a significant increase in biofilm density and extracellular hydrogen peroxide by wild type E. faecalis. By site-directed mutagenesis gene products encoded in the SDMH operon were shown to be necessary for the selenium-dependent biofilm formation as well as extracellular hydrogen peroxide production. This biofilm and peroxide phenotype is inhibited both by tungsten or auranofin in wild type E. faecalis suggesting the SDMH is a necessary enzyme for selenium-dependent biofilm and peroxide formation. These results show that the gene products encoded within the SDMH operon are necessary for a selenium-dependent biofilm formation as well as extracellular hydrogen peroxide production. These mutants will provide the basis for defining the synthesis of the labile selenium cofactor and allow for an expanded understanding of the biological use of selenium
Modeling natural emissions in the Community Multiscale Air Quality (CMAQ) model – Part 2: Modifications for simulating natural emissions
The Community Multiscale Air Quality (CMAQ) model version 4.6 has been revised with regard to the representation of chlorine (HCl, ClNO<sub>2</sub>) and sulfur (dimethylsulfide, or DMS, and H<sub>2</sub>S), and evaluated against observations and earlier published models. Chemistry parameterizations were based on published reaction kinetic data and a recently developed cloud chemistry model that includes heterogeneous reactions of organic sulfur compounds. Evaluation of the revised model was conducted using a recently enhanced data base of natural emissions that includes ocean and continental sources of DMS, H<sub>2</sub>S, chlorinated gases and lightning NO<sub>x</sub> for the continental United States and surrounding regions. Results using 2002 meteorology and emissions indicated that most simulated "natural" (plus background) chemical and aerosol species exhibit the expected seasonal variations at the surface. Ozone exhibits a winter and early spring maximum consistent with ozone data and an earlier published model. Ozone distributions reflect the influences of atmospheric dynamics and pollutant background levels imposed on the CMAQ simulation by boundary conditions derived from a global model. A series of model experiments reveals that the consideration of gas-phase organic sulfur chemistry leads to sulfate aerosol increases over most of the continental United States. Cloud chemistry parameterization changes result in widespread decreases in SO<sub>2</sub> across the modeling domain and both increases and decreases in sulfate. Most cloud-mediated sulfate increases occurred mainly over the Pacific Ocean (up to about 0.1 μg m<sup>−3</sup>) but also over and downwind from the Gulf of Mexico (including parts of the eastern US). Geographic variations in simulated SO<sub>2</sub> and sulfate are due to the link between DMS/H<sub>2</sub>S and their byproduct SO<sub>2</sub>, the heterogeneity of cloud cover and precipitation (precipitating clouds act as net sinks for SO<sub>2</sub> and sulfate), and the persistence of cloud cover (the largest relative sulfate increases occurred over the persistently cloudy Gulf of Mexico and western Atlantic Ocean). Overall, the addition of organic sulfur chemistry increased hourly surface sulfate levels by up to 1–2 μg m<sup>−3</sup> but reduced sulfate levels in the vicinity of high SO<sub>2</sub> emissions (e.g., wildfires). Simulated surface levels of DMS compare reasonably well with observations in the marine boundary layer where DMS oxidation product levels are lower than observed. This implies either a low bias in model oxidation rates of organic sulfur species or a low bias in the boundary conditions for DMS oxidation products. This revised version of CMAQ provides a tool for realistically simulating the influence of natural emissions on air quality
Quantitative genetics of growth traits in the edible snail, Helix aspersa Müller
Genetic parameters of adult weight, age at maturity (adult age), weight after hibernation and relative loss of weight during hibernation were estimated in a population of edible snails (Helix aspersa Müller). Eight thousand four hundred and eighthy three animals were sampled from 143 pairs for adult weight, 4 333 from 87 pairs for adult age and 2 256 from 123 pairs for traits after hibernation. An animal model taking into account all the relationships was used to estimate genetic parameters. Estimates were also computed from the covariances between full-sibs and parent offspring regressions to assess possible non-additive genetic effects. Heritabilities were high except for relative loss of weight during hibernation. Estimates from the animal model were 0.48 ± 0.04 for adult weight, 0.40 ± 0.05 for adult age, 0.40 ± 0.05 for weight after hibernation and 0.12 ± 0.03 for relative loss of weight during hibernation. Adult weight and adult age were neither phenotypically nor genetically correlated (0.05 and 0.003 ± 0.07, respectively). A substantial maternal effect, especially on adult weight was found.Les paramètres génétiques de plusieurs caractères de croissance ont été estimés dans une population d’escargots Petit-Gris (Helix aspersa Müller). Il s’agit du poids adulte, de l’âge à maturité (âge adulte), du poids après hibernation et de la perte relative de poids lors de l’hibernation. Le nombre d’observations collectées se répartit ainsi : 8 483 animaux issus de 143 couples pour le poids adulte, 4 333 issus de 87 couples pour l’âge adulte et 2 256 issus de 123 couples pour les caractères mesurés après hibernation. Afin de tenir compte de toutes les relations de parenté, nous avons utilisé un modèle animal pour estimer les paramètres génétiques. Ils ont également été estimés à partir des covariances entre plein-frères et de la régression parents-descendants. Cela nous a permis de discuter des effets génétiques non additifs. Tous les caractères sauf la perte de poids relative lors de l’hibernation révèlent des héritabilités élevées. Les estimations issues du modèle animal sont de 0,48 ± 0,04 pour le poids adulte, 0,40 ± 0,05 pour l’âge adulte, 0,40 ± 0,05 pour le poids après hibernation et 0, 12 ± 0 03 pour la perte relative de poids lors de l’hibernation. Il n’y a pas de corrélation (ni phénotypique, ni génétique) significative entre le poids et l’âge adultes (0,05 et 0,003 ± 0,07, respectivement). Nous avons également mis en évidence un effet maternel important, en particulier sur le poids adulte
Experimental investigation of planar ion traps
Chiaverini et al. [Quant. Inf. Comput. 5, 419 (2005)] recently suggested a
linear Paul trap geometry for ion trap quantum computation that places all of
the electrodes in a plane. Such planar ion traps are compatible with modern
semiconductor fabrication techniques and can be scaled to make compact, many
zone traps. In this paper we present an experimental realization of planar ion
traps using electrodes on a printed circuit board to trap linear chains of tens
of 0.44 micron diameter charged particles in a vacuum of 15 Pa (0.1 torr). With
these traps we address concerns about the low trap depth of planar ion traps
and develop control electrode layouts for moving ions between trap zones
without facing some of the technical difficulties involved in an atomic ion
trap experiment. Specifically, we use a trap with 36 zones (77 electrodes)
arranged in a cross to demonstrate loading from a traditional four rod linear
Paul trap, linear ion movement, splitting and joining of ion chains, and
movement of ions through intersections. We further propose an additional DC
biased electrode above the trap which increases the trap depth dramatically,
and a novel planar ion trap geometry that generates a two dimensional lattice
of point Paul traps.Comment: 11 pages, 20 figure
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