ANALGETIC ASSAY OF TREMBESI LEAVES (Samanea saman (Jacq.) Merr.)

Background: Trembesi (Samanea saman (Jacq.) Merr.) is a plant of the genus Samanea spread in tropical areainclude Indonesia. This plant have some chemical ingredients such as flavonoids, alkaloids, tannins, saponins,  and steroids or triterpenoids. Objective: To know the analgesic activity and the effective dose of ethanol extract of leaves of trembesi  in white male mice (Mus musculus L.). Method: This research is experimental with Post-Test Only Control Group Design techniques. Analgesic activity test was performed by chemical induction method using acetic acid in vivo with a negative control treatment (CMC-Na 0,5%), a positive control (aspirin 500mg), dose of ethanol extract of trembesi 150, 300 and 600 mg/kgBB. Data were analyzed with Hendersoth and Forsaith equation to determine the amount of stretching by mice. Analysis by One Way ANOVA test and LSD test. Results: The results showed the analgesic power percent ethanol extract of trembesi leaves are respectively 16.6%, 45.8% and 69.6%. Conclusions: The dose of ethanol extract of trembesi leaves have analgesic activity with the most effective dose is 600 mg/kgBB is equivalent to 500mg of aspirin (p=0.560)

ANALGETIC ASSAY OF TREMBESI LEAVES (Samanea saman (Jacq.) Merr.)

Background: Trembesi (Samanea saman (Jacq.) Merr.) is a plant of the genus Samanea spread in tropical areainclude Indonesia. This plant have some chemical ingredients such as flavonoids, alkaloids, tannins, saponins,  and steroids or triterpenoids. Objective: To know the analgesic activity and the effective dose of ethanol extract of leaves of trembesi  in white male mice (Mus musculus L.). Method: This research is experimental with Post-Test Only Control Group Design techniques. Analgesic activity test was performed by chemical induction method using acetic acid in vivo with a negative control treatment (CMC-Na 0,5%), a positive control (aspirin 500mg), dose of ethanol extract of trembesi 150, 300 and 600 mg/kgBB. Data were analyzed with Hendersoth and Forsaith equation to determine the amount of stretching by mice. Analysis by One Way ANOVA test and LSD test. Results: The results showed the analgesic power percent ethanol extract of trembesi leaves are respectively 16.6%, 45.8% and 69.6%. Conclusions: The dose of ethanol extract of trembesi leaves have analgesic activity with the most effective dose is 600 mg/kgBB is equivalent to 500mg of aspirin (p=0.560)

Uji Aktivitas Tonikum Ekstrak Etanol Daun Seledri (Apium graveolens L.) Metode Natatory Exhaustion

Fatigue syndrome menunjukkan karakter kelelahan yang intens dengan durasi lebih dari enam bulan dan berhubungan dengan berbagai gejala pada pasien. Kelelahan ini tidak pulih setelah tidur malam dan dapat mengganggu aktivitas sehari-hari sebanyak 50% (Fernández et al., 2009). Penggunaan tonikum untuk mengurangi gejala sindrom ini menjadi salah satu pilihan masyarakat. Tujuan penelitian ini adalah untuk mengetahui adanya aktivitas tonikum beserta dosis optimum ekstrak etanol Daun Seledri (Apium graveolens L.) secara in vivo. Metode penelitian yaitu dengan mengujikan ekstrak etanol 96% pada 25 ekor mencit yang terbagi dalam 5 kelompok. Kelompok kontrol negatif CMC Na 0,5%, kontrol positif kafein 100 mg/KgBB, kelompok ekstrak daun seledri 100 mg/KgBB, 200 mg/KgBB dan 400 mg/KgBB diberikan secara peroral. Pengujian dilakukan dengan metode natatory exhaustion. Analisis data dilakukan dengan cara membandingkan waktu berenang sebelum dan sesudah perlakuan dan disajikan dalam presentase. Hasil penelitian menunjukkan ekstrak etanol daun seledri dosis 400 mg/KgBB memiliki aktivitas tonikum paling tinggi sebesar 30,35% sedangkan kontrol positif kafein menunjukkan aktivitas tonikum sebesar 23,44%. Hal ini diduga kerena adanya berbagai senyawa yang dapat bersinergi meningkatkan aktivitas tonikum