Características epidemiológicas y moleculares de <i>Chlamydia psittaci</i> provenientes de 8 casos humanos de psitacosis y de 4 aves relacionadas en la Argentina

In Argentina, the epidemiological and molecular characteristics of Chlamydia psittaci infections are still not sufficiently known. A total of 846 respiratory and 10 ocular samples from patients with suspected human psittacosis were tested for C. psittaci from January 2010 to March 2015. Four samples of birds related to these patients were also studied. Forty-eight samples were positive for C. psittaci by a nested PCR. The molecular characterization of twelve C. psittaci PCR-positive samples received in the National Reference Laboratory INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires, Argentina was performed. Eight positive samples from humans and four from birds were genotyped by ompA gene sequencing. C. psittaci genotype A was found in all human samples and in the related birds. This report contributes to our increasing knowledge of the epidemiological and molecular characteristics of C. psittaci to conduct effective surveillance of its zoonotic infections.En la Argentina, aún no se conocen suficientemente las características epidemiológicas y moleculares de las infecciones por Chlamydia psittaci. Entre enero del 2010 y marzo del 2015 se estudiaron 846 muestras respiratorias y 10 oculares de pacientes con sospecha de psitacosis para la búsqueda de C. psittaci. También se estudiaron 4 muestras de aves relacionadas con estos pacientes. De ese total, 48 muestras fueron positivas para C. psittaci mediante una reacción en cadena de la polimerasa (PCR) anidada. Posteriormente, se realizó en el INEI-ANLIS «Dr. Carlos G. Malbrán» la caracterización molecular de 12 muestras positivas para C. psittaci, 8 de humanos y 4 de aves, que fueron genotipificadas por secuenciación del gen ompA. C. psittaci genotipo A se encontró en todas esas muestras. Este informe contribuye a mejorar nuestro conocimiento de las características epidemiológicas y moleculares de C. psittaci para lograr una vigilancia efectiva de la zoonosis que produce.Facultad de Ciencias Veterinaria

Exercise Therapy for Fibromyalgia

Fibromyalgia syndrome, a chronic condition typically characterized by widespread pain, nonrestorative sleep, fatigue, cognitive dysfunction, and other somatic symptoms, negatively impacts physical and emotional function and reduces quality of life. Exercise is commonly recommended in the management of people with fibromyalgia, and interest in examining exercise benefits for those with the syndrome has grown substantially over the past 25 years. Research supports aerobic and strength training to improve physical fitness and function, reduce fibromyalgia symptoms, and improve quality of life. However, other forms of exercise (e.g., tai chi, yoga, Nordic walking, vibration techniques) and lifestyle physical activity also have been investigated to determine their effects. This paper highlights findings from recent randomized controlled trials and reviews of exercise for people with fibromyalgia, and includes information regarding factors that influence response and adherence to exercise to assist clinicians with exercise and physical activity prescription decision-making to optimize health and well-being

Rehabilitation at the Time of Pandemic: Patient Journey Recommendations

Purpose: The World Health Organization (WHO) declared severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) a pandemic in March 2020, causing almost 3.5 million coronavirus disease (COVID-19) related deaths worldwide. The COVID-19 pandemic has imposed a significant burden on healthcare systems, economies, and social systems in many countries around the world. The access and delivery of rehabilitation care were severely disrupted, and patients have faced several challenges during the COVID-19 outbreak. These challenges include addressing new functional impairments faced by survivors of COVID-19 and infection prevention to avoid the virus spread to healthcare workers and other patients not infected with COVID-19. In this scoping review, we aim to develop rehabilitation recommendations during the COVID-19 pandemic across the continuum of rehabilitation care. Materials and Methods: Established frameworks were used to guide the scoping review methodology. Medline, Embase, Pubmed, CINAHL databases from inception to August 1, 2020, and prominent rehabilitation organizations’ websites were searched. Study Selection: We included articles and reports if they were focused on rehabilitation recommendations for COVID-19 survivors or the general population at the time of the COVID-19 pandemic. Data Extraction: Two of our team members used the pre-tested data extraction form to extract data from included full-text articles. The strength and the quality of the extracted recommendations were evaluated by two reviewers using the GRADE (Grading of Recommendations, Assessment, Development and Evaluation) approach. Results: We retrieved 6,468 citations, of which 2,086 were eligible after removing duplicates. We excluded 1,980 citations based on the title and the abstract. Of the screened full-text articles, we included 106 studies. We present recommendations based on the patient journey at the time of the pandemic. We assessed the evidence to be of overall fair quality and strong for the recommendations. Conclusion: We have combined the latest research results and accumulated expert opinions on rehabilitation to develop acute and post-acute rehabilitation recommendations in response to the global COVID-19 pandemic. Further updates are warranted in order to incorporate the emerging evidence into rehabilitation guidelines

Características epidemiológicas y moleculares de <i>Chlamydia psittaci</i> provenientes de 8 casos humanos de psitacosis y de 4 aves relacionadas en la Argentina

In Argentina, the epidemiological and molecular characteristics of Chlamydia psittaci infections are still not sufficiently known. A total of 846 respiratory and 10 ocular samples from patients with suspected human psittacosis were tested for C. psittaci from January 2010 to March 2015. Four samples of birds related to these patients were also studied. Forty-eight samples were positive for C. psittaci by a nested PCR. The molecular characterization of twelve C. psittaci PCR-positive samples received in the National Reference Laboratory INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires, Argentina was performed. Eight positive samples from humans and four from birds were genotyped by ompA gene sequencing. C. psittaci genotype A was found in all human samples and in the related birds. This report contributes to our increasing knowledge of the epidemiological and molecular characteristics of C. psittaci to conduct effective surveillance of its zoonotic infections.En la Argentina, aún no se conocen suficientemente las características epidemiológicas y moleculares de las infecciones por Chlamydia psittaci. Entre enero del 2010 y marzo del 2015 se estudiaron 846 muestras respiratorias y 10 oculares de pacientes con sospecha de psitacosis para la búsqueda de C. psittaci. También se estudiaron 4 muestras de aves relacionadas con estos pacientes. De ese total, 48 muestras fueron positivas para C. psittaci mediante una reacción en cadena de la polimerasa (PCR) anidada. Posteriormente, se realizó en el INEI-ANLIS «Dr. Carlos G. Malbrán» la caracterización molecular de 12 muestras positivas para C. psittaci, 8 de humanos y 4 de aves, que fueron genotipificadas por secuenciación del gen ompA. C. psittaci genotipo A se encontró en todas esas muestras. Este informe contribuye a mejorar nuestro conocimiento de las características epidemiológicas y moleculares de C. psittaci para lograr una vigilancia efectiva de la zoonosis que produce.Facultad de Ciencias Veterinaria

Production system and value chain in oregano (Origanum sp.) cultivation in the province of Córdoba (Argentina)

El objetivo de esta revisión fue realizar un análisis e identificación de la problemática del Sistema de Producción y de la Cadena de Valor del Orégano en la zona del Valle de Traslasierra de la Provincia de Córdoba. En la región, se cultivan ecotipos tradicionales como Criollo, Chileno II y Compacto, y nuevos como el ?Serrano Cordobés?, ?Flor Rosa? y ?Rosa Fuerte?. El Valle Traslasierra de la Provincia de Córdoba es agroecológicamente muy apto para la producción, y para incrementar la producción del cultivo de orégano se debe optimizar el manejo agronómico de los distintos ecotipos. Los estudios ecofisiológicos realizados hasta el momento en la región sugieren que los ecotipos de arquitectura erecta (Criollo) se comportan como un idiotipo más productivo que el rastrero (Compacto). Se citan por primera vez especies fitófagas de lepidópteros como Achira bifidalis (F), Rachiplusia nu (Guenée) y Spodoptera frugiperda (Smith) entre otras, además de la presencia de distintos géneros de nemátodos. Como enfermedades, se encontró la presencia de Fusarium spp y Phomopsis sp; roya y síntomas de virosis. La cadena de valor agroalimentaria se caracterizó por poseer pequeñas explotaciones que, en su mayoría, carecieron de título de propiedad, con todo lo que esto implica. Es necesario generar asociaciones activas de productores, de manera de concentrar el poder de comercialización y de organización.The aim of the present review was to analyze and identify the problems associated with the Production System and Chain Value of Oregano in the area of Traslasierra Valley, province of Córdoba. Traditional ecotypes, such as Criollo, Chileno II and Compacto, are cultivated in the region, as well as new ecotypes such as "Serrano Cordobés", "Flor Rosa" and "Rosa Fuerte". The Traslasierra Valley of Córdoba is a very suitable area for the production and for increasing the production of oregano. However, the agricultural management of the different oregano ecotypes should be optimized. Ecophysiological studies conducted to date in the region suggest that the ecotypes of erect architecture ("Criollo") behave as more productive than the creeping one ("Compacto"). Phytophagous lepidopteran species, such as Achira bifidalis (F), Rachiplusia nu (Guenée) and Spodoptera frugiperda (Smith), among others, as well as the presence of different nematode genera are mentioned for the first time. The diseases detected included Fusarium spp., Phomopsis sp, rust, and virus symptoms. The agricultural food chain value is characterized by small farms where most of the owners lacked a property title, which has adverse consequences. Active producers associations are necessary to concentrate trade and organization possibilities.Fil: Argüello, J. A.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Núñez, S. B.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Davidenco, Vanina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Suárez, D. A.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Seisdedos, L.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Baigorria, M. C.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: la Porta, Norma Cristina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Ruiz, G.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Yossen, Viviana Ester. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentin

Production system and value chain in oregano (Origanum sp.) cultivation in the province of Córdoba (Argentina)

El objetivo de esta revisión fue realizar un análisis e identificación de la problemática del Sistema de Producción y de la Cadena de Valor del Orégano en la zona del Valle de Traslasierra de la Provincia de Córdoba. En la región, se cultivan ecotipos tradicionales como Criollo, Chileno II y Compacto, y nuevos como el ?Serrano Cordobés?, ?Flor Rosa? y ?Rosa Fuerte?. El Valle Traslasierra de la Provincia de Córdoba es agroecológicamente muy apto para la producción, y para incrementar la producción del cultivo de orégano se debe optimizar el manejo agronómico de los distintos ecotipos. Los estudios ecofisiológicos realizados hasta el momento en la región sugieren que los ecotipos de arquitectura erecta (Criollo) se comportan como un idiotipo más productivo que el rastrero (Compacto). Se citan por primera vez especies fitófagas de lepidópteros como Achira bifidalis (F), Rachiplusia nu (Guenée) y Spodoptera frugiperda (Smith) entre otras, además de la presencia de distintos géneros de nemátodos. Como enfermedades, se encontró la presencia de Fusarium spp y Phomopsis sp; roya y síntomas de virosis. La cadena de valor agroalimentaria se caracterizó por poseer pequeñas explotaciones que, en su mayoría, carecieron de título de propiedad, con todo lo que esto implica. Es necesario generar asociaciones activas de productores, de manera de concentrar el poder de comercialización y de organización.The aim of the present review was to analyze and identify the problems associated with the Production System and Chain Value of Oregano in the area of Traslasierra Valley, province of Córdoba. Traditional ecotypes, such as Criollo, Chileno II and Compacto, are cultivated in the region, as well as new ecotypes such as "Serrano Cordobés", "Flor Rosa" and "Rosa Fuerte". The Traslasierra Valley of Córdoba is a very suitable area for the production and for increasing the production of oregano. However, the agricultural management of the different oregano ecotypes should be optimized. Ecophysiological studies conducted to date in the region suggest that the ecotypes of erect architecture ("Criollo") behave as more productive than the creeping one ("Compacto"). Phytophagous lepidopteran species, such as Achira bifidalis (F), Rachiplusia nu (Guenée) and Spodoptera frugiperda (Smith), among others, as well as the presence of different nematode genera are mentioned for the first time. The diseases detected included Fusarium spp., Phomopsis sp, rust, and virus symptoms. The agricultural food chain value is characterized by small farms where most of the owners lacked a property title, which has adverse consequences. Active producers associations are necessary to concentrate trade and organization possibilities.Fil: Argüello, J. A.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Núñez, S. B.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Davidenco, Vanina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Suárez, D. A.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Seisdedos, L.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Baigorria, M. C.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: la Porta, Norma Cristina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Ruiz, G.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Yossen, Viviana Ester. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentin

Increasing arterial blood pressure with norepinephrine does not improve microcirculatory blood flow: a prospective study

Introduction Our goal was to assess the effects of titration of a norepinephrine infusion to increasing levels of mean arterial pressure (MAP) on sublingual microcirculation. Methods Twenty septic shock patients were prospectively studied in two teaching intensive care units. The patients were mechanically ventilated and required norepinephrine to maintain a mean arterial pressure (MAP) of 65 mmHg. We measured systemic hemodynamics, oxygen transport and consumption (DO2 and VO2), lactate, albumin-corrected anion gap, and gastric intramucosal-arterial PCO2 difference (Delta PCO2). Sublingual microcirculation was evaluated by sidestream darkfield (SDF) imaging. After basal measurements at a MAP of 65 mmHg, norepinephrine was titrated to reach a MAP of 75 mmHg, and then to 85 mmHg. Data were analyzed using repeated measurements ANOVA and Dunnett test. Linear trends between the different variables and increasing levels of MAP were calculated. Results Increasing doses of norepinephrine reached the target values of MAP. The cardiac index, pulmonary pressures, systemic vascular resistance, and left and right ventricular stroke work indexes increased as norepinephrine infusion was augmented. Heart rate, DO2 and VO2, lactate, albumin-corrected anion gap, and Delta PCO2 remained unchanged. There were no changes in sublingual capillary microvascular flow index (2.1 +/- 0.7, 2.2 +/- 0.7, 2.0 +/- 0.8) and the percent of perfused capillaries (72 +/- 26, 71 +/- 27, 67 +/- 32%) for MAP values of 65, 75, and 85 mmHg, respectively. There was, however, a trend to decreased capillary perfused density (18 +/- 10,17 +/- 10,14 +/- 2 vessels/mm(2), respectively, ANOVA P = 0.09, linear trend P = 0.045). In addition, the changes of perfused capillary density at increasing MAP were inversely correlated with the basal perfused capillary density (R-2 = 0.95, P < 0.0001). Conclusions Patients with septic shock showed severe sublingual microcirculatory alterations that failed to improve with the increases in MAP with norepinephrine. Nevertheless, there was a considerable interindividual variation. Our results suggest that the increase in MAP above 65 mmHg is not an adequate approach to improve microcirculatory perfusion and might be harmful in some patient

Incidence and prevalence of dementia in linked administrative health data in Saskatchewan, Canada: a retrospective cohort study.

Determining the epidemiology of dementia among the population as a whole in specific jurisdictions - including the long-term care population-is essential to providing appropriate care. The objectives of this study were to use linked administrative databases in the province of Saskatchewan to determine the 12-month incidence and prevalence of dementia for the 2012/13 period (1) among individuals aged 45 and older in the province of Saskatchewan, (2) according to age group and sex, and (3) according to diagnosis code and other case definition criteria