Pol I DNA polymerases stimulate DNA end-joining by Escherichia coli DNA ligase

© 2018 Klenow and Klentaq are the large fragment domains of the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus, respectively. Herein, we show that both polymerases can significantly stimulate complementary intermolecular end-joining ligations by E.coli DNA ligase when the polymerases are present at concentrations lower than that of the DNA substrates. In contrast, high polymerase concentrations relative to the DNA substrates inhibit the intermolecular ligation activity of DNA ligase. Neither polymerase was able to stimulate the DNA ligase from T4 bacteriophage. Additionally, nick-closure by E. coli DNA ligase (but not T4 ligase) is slightly stimulated by both polymerases, but only at about 10% of the magnitude seen for end-joining enhancement. The data represent one of the first observations of direct polymerase-ligase interactions in prokaryotes, and suggest that the polymerases stabilize the associated DNA ends during intermolecular ligation, and that such a complex can be taken advantage of by some, but not all, DNA ligases

The roles of aldehyde dehydrogenases (ALDHs) in the PDH bypass of Arabidopsis

<p>Abstract</p> <p>Background</p> <p>Eukaryotic aldehyde dehydrogenases (ALDHs, EC 1.2.1), which oxidize aldehydes into carboxylic acids, have been classified into more than 20 families. In mammals, Family 2 ALDHs detoxify acetaldehyde. It has been hypothesized that plant Family 2 ALDHs oxidize acetaldehyde generated via ethanolic fermentation, producing acetate for acetyl-CoA biosynthesis via acetyl-CoA synthetase (ACS), similar to the yeast pathway termed the "pyruvate dehydrogenase (PDH) bypass". Evidence for this pathway in plants has been obtained from pollen.</p> <p>Results</p> <p>To test for the presence of the PDH bypass in the sporophytic tissue of plants, Arabidopsis plants homozygous for mutant alleles of all three Family 2 ALDH genes were fed with ¹⁴C-ethanol along with wild type controls. Comparisons of the incorporation rates of ¹⁴C-ethanol into fatty acids in mutants and wild type controls provided direct evidence for the presence of the PDH bypass in sporophytic tissue. Among the three Family 2 ALDHs, one of the two mitochondrial ALDHs (ALDH2B4) appears to be the primary contributor to this pathway. Surprisingly, single, double and triple ALDH mutants of Arabidopsis did not exhibit detectable phenotypes, even though a Family 2 ALDH gene is required for normal anther development in maize.</p> <p>Conclusion</p> <p>The PDH bypass is active in sporophytic tissue of plants. Blocking this pathway via triple ALDH mutants does not uncover obvious visible phenotypes.</p

Elemental Abundances of Blue Compact Dwarfs from mid-IR Spectroscopy with Spitzer

We present a study of elemental abundances in a sample of thirteen Blue Compact Dwarf (BCD) galaxies, using the $\sim$10--37$\mu$m high resolution spectra obtained with Spitzer/IRS. We derive the abundances of neon and sulfur for our sample using the infrared fine-structure lines probing regions which may be obscured by dust in the optical and compare our results with similar infrared studies of starburst galaxies from ISO. We find a good correlation between the neon and sulfur abundances, though sulfur is under-abundant relative to neon with respect to the solar value. A comparison of the elemental abundances (neon, sulfur) measured from the infrared data with those derived from the optical (neon, sulfur, oxygen) studies reveals a good overall agreement for sulfur, while the infrared derived neon abundances are slightly higher than the optical values. This indicates that either the metallicities of dust enshrouded regions in BCDs are similar to the optically accessible regions, or that if they are different they do not contribute substantially to the total infrared emission of the host galaxy.Comment: 11 pages, 6 figures, accepted by Ap

The Energetics of Molecular Gas in NGC 891 from H_2 and Far-infrared Spectroscopy

We have studied the molecular hydrogen energetics of the edge-on spiral galaxy NGC 891, using a 34 position map in the lowest three pure rotational H_2 lines observed with the Spitzer Infrared Spectrograph. The S(0), S(1), and S(2) lines are bright with an extinction-corrected total luminosity of ~2.8 × 10^7 L_☉, or 0.09% of the total-infrared luminosity of NGC 891. The H_2 line ratios are nearly constant along the plane of the galaxy—we do not observe the previously reported strong drop-off in the S(1)/S(0) line intensity ratio in the outer regions of the galaxy, so we find no evidence for the very massive cold CO-free molecular clouds invoked to explain the past observations. The H_2 level excitation temperatures increase monotonically indicating that there is more than one component to the emitting gas. More than 99% of the mass is in the lowest excitation (T_(ex) ~ 125 K) "warm" component. In the inner galaxy, the warm H_2 emitting gas is ~16% of the CO(1-0)-traced cool molecular gas, while in the outer regions the fraction is twice as high. This large mass of warm gas is heated by a combination of the far-UV photons from stars in photodissociation regions (PDRs) and the dissipation of turbulent kinetic energy. Including the observed far-infrared [O I] and [C II] fine-structure line emission and far-infrared continuum emission in a self-consistent manner to constrain the PDR models, we find essentially all of the S(0) and most (70%) of the S(1) line arise from low excitation PDRs, while most (80%) of the S(2) and the remainder of the S(1) line emission arise from low-velocity microturbulent dissipation

On the binary input Gaussian wiretap channel with/without output quantization

Abstract: In this paper, we investigate the effect of output quantization on the secrecy capacity of the binary-input Gaussian wiretap channel. As a result, a closed-form expression with infinite summation terms of the secrecy capacity of the binary-input Gaussian wiretap channel is derived for the case when both the legitimate receiver and the eavesdropper have unquantized outputs. In particular, computable tight upper and lower bounds on the secrecy capacity are obtained. Theoretically, we prove that when the legitimate receiver has unquantized outputs while the eavesdropper has binary quantized outputs, the secrecy capacity is larger than that when both the legitimate receiver and the eavesdropper have unquantized outputs or both have binary quantized outputs. Further, numerical results show that in the low signal-to-noise ratio (SNR) (of the main channel) region, the secrecy capacity of the binary input Gaussian wiretap channel when both the legitimate receiver and the eavesdropper have unquantized outputs is larger than the capacity when both the legitimate receiver and the eavesdropper have binary quantized outputs; as the SNR increases, the secrecy capacity when both the legitimate receiver and the eavesdropper have binary quantized outputs tends to overtake

The association of household food security, household characteristics and school environment with obesity status among off-reserve first nations and métis children and youth in Canada: Results from the 2012 aboriginal peoples survey

Introduction: Indigenous children are twice as likely to be classified as obese and three times as likely to experience household food insecurity when compared with non-Indigenous Canadian children. The purpose of this study was to explore the relationship between food insecurity and weight status among Métis and off-reserve First Nations children and youth across Canada. Methods: We obtained data on children and youth aged 6 to 17 years (n = 6900) from the 2012 Aboriginal Peoples Survey. We tested bivariate relationships using Pearson chi-square tests and used nested binary logistic regressions to examine the food insecurity−weight status relationship, after controlling for geography, household and school characteristics and cultural factors. Results: Approximately 22% of Métis and First Nations children and youth were overweight, and 15% were classified as obese. Over 80% of the sample was reported as food secure, 9% experienced low food security and 7% were severely food insecure. Off-reserve Indigenous children and youth from households with very low food security were at higher risk of overweight or obese status; however, this excess risk was not independent of household socioeconomic status, and was reduced by controlling for household income, adjusted for household size. Negative school environment was also a significant predictor of obesity risk, independent of demographic, household and geographic factors. Conclusion: Both food insecurity and obesity were prevalent among the Indigenous groups studied, and our results suggest that a large proportion of children and youth who are food insecure are also overweight or obese. This study reinforces the importance of including social determinants of health, such as income, school environment and geography, in programs or policies targeting child obesity