Towards ultra-high resolution 3D reconstruction of a whole rat brain from 3D-PLI data

3D reconstruction of the fiber connectivity of the rat brain at microscopic scale enables gaining detailed insight about the complex structural organization of the brain. We introduce a new method for registration and 3D reconstruction of high- and ultra-high resolution (64 $\mu$m and 1.3 $\mu$m pixel size) histological images of a Wistar rat brain acquired by 3D polarized light imaging (3D-PLI). Our method exploits multi-scale and multi-modal 3D-PLI data up to cellular resolution. We propose a new feature transform-based similarity measure and a weighted regularization scheme for accurate and robust non-rigid registration. To transform the 1.3 $\mu$m ultra-high resolution data to the reference blockface images a feature-based registration method followed by a non-rigid registration is proposed. Our approach has been successfully applied to 278 histological sections of a rat brain and the performance has been quantitatively evaluated using manually placed landmarks by an expert.Comment: 9 pages, Accepted at 2nd International Workshop on Connectomics in NeuroImaging (CNI), MICCAI'201

Unoccupied states of individual silver clusters and chains on Ag(111)

Size-selected silver clusters on Ag(111) were fabricated with the tip of a scanning tunneling microscope. Unoccupied electron resonances give rise to image contrast and spectral features which shift toward the Fermi level with increasing cluster size. Linear assemblies exhibit higher resonance energies than equally sized compact assemblies. Density functional theory calculations reproduce the observed energies and enable an assignment of the resonances to hybridized atomic 5s and 5p orbitals with silver substrate states.Comment: 9 pages, 8 figure

Tribological system for cold sheet metal forming based on volatile lubricants and laser structured surfaces

AbschlussberichtA novel tribological system has been developed, in which volatile lubricants (carbon dioxide - CO2 or nitrogen- N2) are used as a substitute for mineral oil-based lubricants in deep drawing processes. This process enables the introduction of an intermediate medium under high pressure through flow-optimized, laser-drilled micro holes into the contact surfaces. This eliminates the need for subsequent, cost-intensive cleaning processes, as the volatile lubricants evaporate without leaving any residue during expansion to ambient pressure. The design of initial micro hole geometries was based on simulations of the flow behaviour of the lubricants passing through, which in turn were validated using pressure reactor tests. In addition, the wetting behaviour of CO2 on relevant surfaces (tool surface and sheet material surface) was investigated experimentally using the captive-bubble-method. Thus, the optimal design of the micro holes (diameter, hole geometry and number of micro holes) could be determined using flat strip drawing tests. The optimal micro hole geometry determined in this way is suited for the use of both CO2 and N2 as volatile lubricant. Furthermore, extensive investigations for the production of the required micro hole geometry by laser drilling were carried out. The fundamentals for drilling micro holes in steel with high aspect ratios could be developed using an ultrashort pulsed research laser with very high pulse energy. Further experiments were conducted using an ultrashort pulsed prototype laser of the kW-class specially developed to increase productivity when drilling a multitude of micro holes with higher average laser power. The novel tribological system has been characterised by means of strip drawing tests and stretch bending tests. For both, CO2 (liquid) and N2 (gaseous), relatively low friction values could be achieved compared to conventional lubricants. It could be shown that deep drawing with both CO2 and N2 as dry lubricants is possible. Here, usage of the volatile lubricants not only allows the replacement of mineral oil based lubricants, but even improves the tribological system with regard to frictional forces in sheet metal forming. The feasibility of the new tribological system has been proven by performing deep drawing tests of rectangular cups. These tests showed a significantly enlarged process window of the forming process, which emphasise the tremendous potential of this new tribological system.12816

Interaction-induced effects in the nonlinear coherent response of quantum-well excitons

Interaction-induced processes are studied using the third-order nonlinear polarization created in polarization-dependent four-wave-mixing experiments (FWM) on a ZnSe single quantum well. We discuss their influence by a comparison of the experimental FWM with calculations based on extended optical Bloch equations including local-field effects, excitation-induced dephasing, and biexciton formation. The investigations show that, for copolarized input fields, excitation-induced dephasing is the dominant FWM mechanism, followed by the conventional density-grating FWM process, biexcitonic contributions, and local-field effects. For cross-linear polarized input fields the excitation-induced dephasing mechanism is canceled so that the conventional density-grating FWM process and biexcitonic contributions are dominating

Mixed biexcitons in single quantum wells

Biexcitonic complexes in a ZnSe single quantum well are investigated by spectrally resolved four-wave mixing (FWM). The formation of heavy-heavy-hole XXh and of mixed heavy-light-hole XXm biexcitons showing binding energies of Δh=4.8meV and Δm=2.8meV is identified by polarization selection rules. The coherent dynamics of the FWM response and the observed FWM intensity ratio between the XXh and XXm biexciton-induced nonlinear signals are in agreement with the solution of an extended optical Bloch equation