29 research outputs found
The GPI anchor of cell-surface proteins is synthesized on the cytoplasmic face of the endoplasmic reticulum.
Full text linkCharacterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease.
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Early lipid intermediates in glycosyl-phosphatidylinositol anchor assembly are synthesized in the ER and located in the cytoplasmic leaflet of the ER membrane bilayer.
No full textGeneClip™ U1 Hairpin Cloning Systems for expression of short hairpin RNAs in vivo
No full textThe GeneClip™ U1 Hairpin Cloning Systems enable cloning and expression of short hairpin RNAs (shRNAs) for RNA interference (RNAi) experiments. The GeneClip™ Systems contain linearized vectors that facilitate cloning of complementary oligonucleotides downstream of a U1 promoter. Upon transfection with pGeneClip™ Vectors containing these cloned sequences, shRNA is produced in the cell, leading to reduction of target gene expression through RNAi. In this article we illustrate use of the GeneClip™ Systems to suppress Renilla luciferase expression in a range of cell types and to suppress p53 expression in both a transient and stable manner
Novel Pyrimidines as Multitarget Protein Tyrosine Kinase Inhibitors for the Treatment of Idiopathic Pulmonary Fibrosis (IPF)
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