Toxicity of Certain Penta-Coordinated Organotin(IV) and Tetra-Coordinated Tin(II) Complexes of Heterocyclic β-Diketones Against the Larvae of Aedes Aegypti (Liston)

Some organotin(IV) and tin(II) complexes of composition R3Sn[R'COC:CON(C6H5)N:CCH3] (where R=C4H9, R'=CH3, C3H5, p-ClC6H4; R=C6H5, R'=C6H5 and p-ClC6H4) and Sn[p-ClC6H4COC:CON(C6H5)N:CCH3]2 were screened for their toxicity against Aedes aegypti larvae. Organotin(IV) complexes were more active than tin(II) complexes

ORIENTAL JOURNAL OF CHEMISTRY Development and Validation of RP-HPLC Method for Simultaneous Determination of Niacin (Extended Release) and Lovastatin in Oral Solid Dosage Form

ABSTRACT A simple, precise and rapid HPLC method has been developed and validated for the estimation of Lovastatin and niacin simultaneously in combined dosage form. Chromatographic separation of the two drugs was performed on a Purospher BDSC8 column (150mm×4.6mmid, 5µm particlesize).The mobile phase used was a mixture of 0.1% v/v triethylamine (pH 5.0), containing 20 mM of ammonium acetate buffer: methanol (30:70% v/v).Detection was performed at 237nm and sharp peaks were obtained forniacin and Lovastatin at retention times of 3.2±0.01 min and.6.4±0.01 min respectively.The calibration curve was linear in the concentration range 100-700µg/ml for niacin 3-18µg/ml for Lovastatin; the correlation coefficients were 0.9991 and 0.9992, respectively. The optimized method showed good performance in terms of specificity, linearity, detection and quantitationlimits, precision and accuracy in accordance with the International Conference on Harmonization (ICH) Q2 (R1) guidelines.This assay was demonstrated to be applicable for routine quantitation of Lovastatin and niacin in tablet dosage form

Isocratic RP-HPLC Method for Simultaneous Separation and Estimation of Zofenopril and Hydrochlorthiazide in Pharmaceutical Dosage Forms

A simple, rapid, sensitive and accurate isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of Zofenopril and Hydrochlorthiazide in combined dosage form. Chromatographic separation of the two drugs was performed on a Purospher BDS C18 column (250 mm × 4.6 mm id, 5 μm particle size). The mobile phase comprising of acetonitrile methanol: 0.02M NaH22PO4 buffer (40:20:40) was delivered at a flow rate of 1.0mL/min. The pH of the mobile phase is adjusted to 7.2 with Sodium hydroxide solution. Detection was performed at 245 nm.The separation was completed within 10 min and the retention time of hydrochlorthiazide is 4.62 and Zofenopril is 6.86 min respectively. Calibration curves were linear with R2 between 0.99-1.0 over a concentration range of 100-600 μg/ml for Zofenopril calcium and 50-300 μg/ml for hydrochlorthiazide..The developed method was successfully applied to determ

RP- HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METOLAZONE AND RAMIPRIL IN ORAL SOLID DOSAGE FORM.

A Simple, efficient and reproducible reverse phase high performance liquid chromatographic method was developed and validated for the Simultaneous determination of metolazone (MET) and ramipril (RAM) in combined dosage form. The separation was effected on a Hypersil ODS C18 column (250 mm x 4.6 mm;5µ) using a mobile phase mixture of 30mM sodium dihydrogen phosphate buffer adjusted to pH 2.5.0 with orthophosphoric acid and acetonitrile in a ratio of 40:60 v/v at a flow rate of 1.0ml/min. The detection was made at 242 nm. The retention time of metolazone and ramipril was found to be 4.140 ± 0.007min and 7.007 ± 0.006min. Calibration curve was linear over the concentration range of 10-70 µg/ml for bothMetolazone and Ramipril.All the analytical validation parameters were determined and found in the limit as per ICH guidelines, which indicates the validity of the method. The developed method is also found to be precise, accurate, specific, robust and rapid for the simultaneous determination of Metolazone and Ramipril in tablet dosage forms