157 research outputs found
Thymidine Suicide Assay of Exogenous Spleen Colony Forming Units: Interfering Influence of the Chemical Cytotoxicity of 3H-thymidine Preparations
Regeneration of Murine Erythropoiesis in Individual Bone Marrow Regions (Bones) and Spleen in Lethally Irradiated Marrow Graft Recipients
Use of bio-loggers to characterize red fox behavior with implications for studies of magnetic alignment responses in free-roaming animals
Spin-locking in low-frequency reaction yield detected magnetic resonance
The purported effects of weak magnetic fields on various biological systems from animal magnetoreception to human health have
generated widespread interest and sparked much controversy in the past decade. To date the only well established mechanism
by which the rates and yields of chemical reactions are known to be influenced by magnetic fields is the radical pair mechanism,
based on the spin-dependent reactivity of radical pairs. A diagnostic test for the operation of the radical pair mechanism was
proposed by Henbest et al. [J. Am. Chem. Soc., 2004, 126, 8102] based on the combined effects of weak static magnetic
fields and radiofrequency oscillating fields in a reaction yield detected magnetic resonance experiment. Here we investigate
the effects on radical pair reactions of applying relatively strong oscillating fields, both parallel and perpendicular to the static
field. We demonstrate the importance of understanding the effect of the strength of the radiofrequency oscillating field; our
experiments demonstrate that there is an optimal oscillating field strength above which the observed signal decreases in intensity
and eventually inverts. We establish the correlation between the onset of this effect and the hyperfine structure of the radicals
involved, and identify the existence of ‘overtone’ type features appearing at multiples of the expected resonance field positio
Pollution of Flooded Arable Soils with Heavy Metals and Polycyclic Aromatic Hydrocarbons (PAHs)
Nuclear MET requires ARF and is inhibited by carbon nanodots through binding to phospho-tyrosine in prostate cancer
Nuclear receptor tyrosine kinases (nRTKs) are aberrantly upregulated in many types of cancers, but the regulation of nRTK remains unclear. We previously showed androgen deprivation therapy (ADT) induces nMET in castration-resistant prostate cancer (CRPC) specimens. Through gene expression microarray profiles reanalysis, we identified that nMET signaling requires ARF for CRPC growth in Pten/Trp53 conditional knockout mouse model. Accordingly, aberrant MET/nMET elevation correlates with ARF in human prostate cancer (PCa) specimens. Mechanistically, ARF elevates nMET through binding to MET cytoplasmic domain to stabilize MET. Furthermore, carbon nanodots resensitize cancer cells to MET inhibitors through DNA damage response. The inhibition of phosphorylation by carbon nanodots was identified through binding to phosphate group of phospho-tyrosine via computational calculation and experimental assay. Thus, nMET is essential to precision therapy of MET inhibitor. Our findings reveal for the first time that targeting nMET axis by carbon nanodots can be a novel avenue for overcoming drug resistance in cancers especially prostate cancer
Hematological and morphometric blood value of four cultured species of economically important tropical foodfish
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