Leech Parasitism of the Gulf Coast Box Turtle, Terrapene carolina major (Testudines:Emydidae) in Mississippi, USA

Ten leeches were collected from a Gulf Coast box turtle, Terrapene carolina major, found crossing a road in Gulfport, Harrison County, Mississippi, USA. Eight of the leeches were identified as Placobdella multilineata and 2 were identified as Helobdella europaea. This represents the second vouchered report of leeches from a box turtle. Helobdella europaea is reported for the first time associated with a turtle and for the second time from the New World

Characterisation of Cryogenic Material Properties of 3D-Printed Superconducting Niobium using a 3D Lumped Element Microwave Cavity

We present an experimental characterisation of the electrical properties of 3D-printed Niobium. The study was performed by inserting a 3D-printed Nb post inside an Aluminium cylindrical cavity, forming a 3D lumped element re-entrant microwave cavity resonator. The resonator was cooled to temperatures below the critical temperature of Niobium (9.25K) and then Aluminium (1.2K), while measuring the quality factors of the electromagnetic resonances. This was then compared with finite element analysis of the cavity and a measurement of the same cavity with an Aluminium post of similar dimensions and frequency, to extract the surface resistance of the Niobium post. The 3D-printed Niobium exhibited a transition to the superconducting state at a similar temperature to the regular Niobium, as well as a surface resistance of $3.1\times10^{-4}$ $\Omega$. This value was comparable to many samples of traditionally machined Niobium previously studied without specialised surface treatment. Furthermore, this study demonstrates a simple new method for characterizing the material properties of a relatively small and geometrically simple sample of superconductor, which could be easily applied to other materials, particularly 3D-printed materials. Further research and development in additive manufacturing may see the application of 3D-printed Niobium in not only superconducting cavity designs, but in the innovative technology of the future.Comment: 5 pages, 4 figure

Expression of Ifnlr1 on intestinal epithelial cells is critical to the antiviral effects of IFN-lambda against norovirus and reovirus

Lambda interferon (IFN-λ) has potent antiviral effects against multiple enteric viral pathogens, including norovirus and rotavirus, in both preventing and curing infection. Because the intestine includes a diverse array of cell types, however, the cell(s) upon which IFN-λ acts to exert its antiviral effects is unclear. Here, we sought to identify IFN-λ-responsive cells by generation of mice with lineage-specific deletion of the receptor for IFN-λ, Ifnlr1. We found that expression of IFNLR1 on intestinal epithelial cells (IECs) in the small intestine and colon is required for enteric IFN-λ antiviral activity. IEC Ifnlr1 expression also determines the efficacy of IFN-λ in resolving persistent murine norovirus (MNoV) infection and regulates fecal shedding and viral titers in tissue. Thus, the expression of Ifnlr1 by IECs is necessary for the response to both endogenous and exogenous IFN-λ. We further demonstrate that IEC Ifnlr1 expression is required for the sterilizing innate immune effects of IFN-λ by extending these findings in Rag1-deficient mice. Finally, we assessed whether our findings pertained to multiple viral pathogens by infecting mice specifically lacking IEC Ifnlr1 expression with reovirus. These mice phenocopied Ifnlr1-null animals, exhibiting increased intestinal tissue titers and enhanced reovirus fecal shedding. Thus, IECs are the critical cell type responding to IFN-λ to control multiple enteric viruses. This is the first genetic evidence that supports an essential role for IECs in IFN-λ-mediated control of enteric viral infection, and these findings provide insight into the mechanism of IFN-λ-mediated antiviral activity. IMPORTANCE Human noroviruses (HNoVs) are the leading cause of epidemic gastroenteritis worldwide. Type III interferons (IFN-λ) control enteric viral infections in the gut and have been shown to cure mouse norovirus, a small-animal model for HNoVs. Using a genetic approach with conditional knockout mice, we identified IECs as the dominant IFN-λ-responsive cells in control of enteric virus infection in vivo. Upon murine norovirus or reovirus infection, Ifnlr1 depletion in IECs largely recapitulated the phenotype seen in Ifnlr1(−/−) mice of higher intestinal tissue viral titers and increased viral shedding in the stool. Moreover, IFN-λ-mediated sterilizing immunity against murine norovirus requires the capacity of IECs to respond to IFN-λ. These findings clarify the mechanism of action of this cytokine and emphasize the therapeutic potential of IFN-λ for treating mucosal viral infections

Parasites of the Spotted Sucker, Minytrema melanops (Cypriniformes: Catostomidae) from Arkansas and Oklahoma

During October 2015, March and April 2016 and again between March and April 2017, 15 Spotted Sucker (Minytrema melanops) were collected from sites in the Ouachita (n = 5), Red (n = 1), and St. Francis (n = 5) river drainages, Arkansas, and the Arkansas River drainage, Oklahoma (n = 4), and examined for protozoan and metazoan parasites. Found were Calyptospora sp., Myxobolus sp., Pseudomurraytrema alabarrum, Biacetabulum banghami, Penarchigetes oklensis, and Acanthocephalus sp. New host and distributional records are documented for these parasites