X-Ray Diffraction Intensities of a Smectic-A Liquid Crystal

Higher-order diffraction from the smectic-A liquid-crystalline phase of cyanooctylbiphenyl (COB) has been measured. The dominant short-range disorder, described by the relative intensities, is in agreement with our estimate of the smectic order parameter based on McMillan's mean-field theory

Pressure Induced Topological Phase Transitions in Membranes

Some highly unusual features of a lipid-water liquid crystal are revealed by high pressure x-ray diffraction, light scattering and dilatometric studies of the lamellar (bilayer $L_{\alpha}$) to nonlamellar inverse hexagonal ($H_{II}$) phase transition. (i) The size of the unit cell of the $H_{II}$ phase increases with increasing pressure. (ii) The transition volume, $\Delta V_{bh}$, decreases and appears to vanish as the pressure is increased. (iii) The intensity of scattered light increases as $\Delta V_{bh}$ decreases. Data are presented which suggest that this increase is due to the formation of an intermediate cubic phase, as predicted by recent theoretical suggestions of the underlying universal phase sequence.Comment: 12 pages, typed using REVTEX 2.

An approach for time-resolved x-ray scattering.

Recent biological optical spectroscopic studies have correlated discrete spectroscopic states with biological function in several systems. One of the challenges of molecular biophysics is to correlate structural changes with these spectroscopic states. From small-angle x-ray scattering one can obtain a key structural parameter, the radius of gyration of solubilized proteins. The method described in this paper would permit determination small changes in the radius using polychromatic synchrotron radiation. The high flux of the storage ring combined with an enhancement factor of approximately 10(4), obtained by removing the requirement for monochromatic radiation, will permit determining the radius on a millisecond time scale. Unlike energy-dispersive methods, this method would use all available energies over a wide range of angles

Phase determination of x-ray reflections for membrane-type systems with constant fluid density.

A new technique for phase determination of X-ray reflections from symmetric structures is presented. This method, involving comparison of intensity data from structures with variable fluid layer thickness and constant fluid electron density, permits computation of phase angles, scaling factors, and origin reflection values independently. Possible sources of error inherent in other methods of phase determination are thereby eliminated. Results of the application of this method to model structures and to myelin data are reported. Advantages of the technique, which tests all possible phase angle combinations in a rapid fashion, are discussed

X-ray scattering from labeled membranes.

We present a new method for the determination of structural parameters in biological membranes. Recording the continuous scattering of heavy-atom labeled membranes and applying elementary Fourier methods we obtain the scattering of the heavy-atom distribution alone. The details of this distribution are explored by developing a simple model and testing for cases relevant to biological membranes. We find that the intensity distribution is highly sensitive to many key parameters. The increased signal from heavy-atom labeling and the use of an improved x-ray system make it possible to record patterns from dilute membrane suspensions. Thus determination of these parameters is possible in the same environment where many membrane biochemical studies are performed. Application of the method is made to a model lipid bilayer membrane, dipalmitoyl phosphatidylcholine by labeling with UO2++ ions. We determine the precise distance between UO2++ layers on either side of the membrane as well as the width of the label on each side. This determination permits estimation of phosphate separation across single labeled bilayers in an aqueous suspension

Etude diffractométrique d'un composé smectique C* sous pression

We present the first X-ray study of a smectic C* liquid crystal as a function of pressure. In the vicinity of the smectic C* ↔ smectic A transition, we show that the variation of the tilt angle, θ, of the molecules in the C* phase can be expressed as a function of pressure by the law : θ = K(P - Pc)β. The critical exponent β we are able to determine is close to 0.5.Nous présentons la première étude diffractométrique d'un cristal liquide smectique C*, en fonction de la pression. Nous montrons qu'au voisinage de la transition smectique C* ↔ smectique A, la variation de l'angle d'inclinaison, θ, des molécules dans la phase C* peut s'exprimer en fonction de la pression par la loi : θ = K(P — Pc) β. L'exposant critique β ainsi déterminé est voisin de 0,5