Inflation in a two 3-form fields scenario

A setting constituted by $\mathbb{N}$ 3-form fields, without any direct interaction between them, minimally coupled to gravity, is introduced in this paper as a framework to study the early evolution of the universe. We focus particularly on the two 3-forms case. An inflationary scenario is found, emerging from the coupling to gravity. More concretely, the fields coupled in this manner exhibit a complex interaction, mediated by the time derivative of the Hubble parameter. Our investigation is supported by means of a suitable choice of potentials, employing numerical methods and analytical approximations. In more detail, the oscillations on the small field limit become correlated, and one field is intertwined with the other. In this type of solution, a varying sound speed is present, together with the generation of isocurvature perturbations. The mentioned features allow to consider an interesting model, to test against observation. It is subsequently shown how our results are consistent with current CMB data (viz.Planck and BICEP2).Comment: Version accepted in JCAP. 22 pages, 12 figures, new refs adde

Non-Gaussianity in multiple three-form field inflation

13 pages, 3 figures13 pages, 3 figures14 pages, 3 figures, discussion added, references updated, version accepted in PRD14 pages, 3 figures, discussion added, references updated, version accepted in PR

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes

Endovascular management of iatrogenic renal vascular injuries complicating percutaneous nephrolithotomy: Role of renal angiography and embolization; an analysis of 159 cases

Objective: To describe the use of renal angiography in the detection of renal vascular injuries following percutaneous nephrolithotomy and to assess the efficacy of endovascular management of these complications. Materials and Methods: This was a retrospective review of 159 consecutive patients over a period of 12 years from 2005 to 2016 who presented with significant post nephrolithotomy hemorrhage and who were evaluated by renal angiography as a first-line diagnostic study, followed by embolization of identified renal vascular injuries in the same session. The parameters assessed for each patient included type of renal vascular injury identified, type of embolic material and therapeutic outcome. Results: Renal vascular lesions identified in 119 patients were treated with embolization with complete resolution of hemorrhage, no further clinical deterioration and preservation of renal function. Conclusions: Renal vascular injury is a rare complication of percutaneous nephrolithotomy. Early renal angiography and selective embolization can play an integral diagnostic and therapeutic role

Theranostic nanoemulsions for macrophage COX-2 inhibition in a murine inflammation model

Targeting macrophages for therapeutic and diagnostic purposes is an attractive approach applicable to multiple diseases. Here, we present a theranostic nanoemulsion platform for simultaneous delivery of an anti-inflammatory drug (celecoxib) to macrophages and monitoring of macrophage migration patterns by optical imaging, as measurement of changes in inflammation. The anti-inflammatory effect of the theranostic nanoemulsions was evaluated in a mouse inflammation model induced with complete Freund\u27s adjuvant (CFA). Nanoemulsions showed greater accumulation in the inflamed vs. control paw, with histology confirming their specific localization in CD68 positive macrophages expressing cyclooxygenase-2 (COX-2) compared to neutrophils. With a single dose administration of the celecoxib-loaded theranostic, we observed a reduction in fluorescence in the paw with time, corresponding to a reduction in macrophage infiltration. Our data strongly suggest that delivery of select agents to infiltrating macrophages can potentially lead to new treatments of inflammatory diseases where macrophage behavior changes are monitored in vivo

Ethyl Gallate: Promising Cytoprotective against HIV-1-Induced Cytopathy and Antiretroviral-Induced Cytotoxicity

Introduction. HIV-1 infection in cell culture is typically characterized by certain cytopathic effects such as vacuolization of cells and development of syncytia, which further lead to cell death. In addition, the majority of drugs during HIV treatment exhibit serious adverse effects in patients, apart from their beneficial role. During the screening of cytoprotective agents to protect the cells from HIV-1-associated cell death and also drug-associated toxicity, antioxidants from a natural source are assumed to be a choice. A well-known antioxidant, ethyl gallate (EG), was selected for cytoprotection studies which have already been proven as an anti-HIV agent. Objective. The main objective of the study was to explore the cytoprotective potential of EG against HIV-1-induced cytopathic effect and antiretroviral drug toxicity. Methods. DPPH free radical scavenging assay was performed with EG to find the effective concentration for antioxidant activity. HIV-1infection-associated cytopathic effects and further rescue by EG were studied in MT-2 lymphocytes by the microscopic method and XTT cytopathic assays. The cellular toxicity of different antiretroviral drugs in different cell lines and the consequent cytoprotective effectiveness of EG were investigated using an MTT cell viability assay. Results. Like ascorbic acid, EG exhibited promising antioxidant activity. HIV-1 infection of MT2 cells induces cell death often referred to as the cytopathic effect. In addition, the usage of antiretroviral drugs also causes severe adverse effects like cytotoxicity. In this context, EG was tested for its cytoprotective properties against HIV-1-induced cytopathic effect and drug-mediated cellular toxicity. EG reclaimed back the MT2 cells from HIV-1-induced cell death. Antiretroviral drugs, such as ritonavir, efavirinz, AZT, and nevirapine, were tested for their toxicity and induced more cell death at higher concentrations in different tissue models such as the liver (THLE-3), lung (AEpiCM), colorectal (HT-29), and brain (U87 MG). Pretreated cells with EG were rescued from the toxic doses of ART. Conclusion. EG was found to be exhibited cytoprotection not only from HIV-1-linked cell death but also from the chemotoxicity of antiretroviral drugs. Evidently, EG could be a cytoprotective supplement in the management of AIDS along with its enormous antioxidant benefits

Binary chemical reaction optimization based feature selection techniques for machine learning classification problems

Feature selection is an important pre-processing technique for dimensionality reduction of high-dimensional data in machine learning (ML) field. In this paper, we propose a binary chemical reaction optimization (BCRO) and a hybrid binary chemical reaction optimization-binary particle swarm optimization (HBCRO-BPSO) based feature selection techniques to optimize the number of selected features and improve the classification accuracy. Three objective functions have been used for the proposed feature selection techniques to compare their performances with a BPSO and advanced binary ant colony optimization (ABACO) along with an implemented GA based feature selection approach called as binary genetic algorithm (BGA). Five ML algorithms including K-nearest neighbor (KNN), logistic regression, Naïve Bayes, decision tree, and random forest are considered for classification tasks. Experimental results tested on eleven benchmark datasets from UCI ML repository show that the proposed HBCRO-BPSO algorithm improves the average percentage of reduction in features (APRF) and average percentage of improvement in accuracy (APIA) by 5.01% and 3.83%, respectively over the existing BPSO based feature selection method; 4.58% and 3.12% over BGA; and 4.15% and 2.27% over ABACO when used with a KNN classifier

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Not AvailableIn rice WA (Wild Abortive) CMS system is commercially used for hybrid seed production. In WA-CMS fertility restoration is governed by two independent and dominant genes namely Rf4 and Rf3. Conventionally, the process of screening for the trait of fertility restoration is by tedious testcross progeny evaluation. In this study, earlier reported SSR markers RM6100 and RM 10313 linked to Rf4 and Rf3, respectively have been utilized to screen one hundred breeding lines and identified that 61 lines to carry both Rf3 and Rf4 genes and these lines can be utilized in hybrid rice breeding as restorers. A set of eighteen restorer lines with different combination of Rf4 and Rf3 were selected for crossing with five CMS lines viz., APMS6A, Pusa 5A, IR58025, IR68897, IR79156 and IR68888 and seventy test cross progenies were evaluated for their fertility restoration based on pollen and spikelet fertility. The hybrids viz., APMS6A X GQ-86, IR 79156A X IR-55778R, APMS 6A X VG-269 and IR 68888A X BR-827- 35 were observed to have more than 90% spikelet fertility. In this study observed that restoration ability varied with different CMS lines hence CMS lines also playing major role achieving higher heterosis.Not Availabl