79 research outputs found

    Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells

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    Cathepsin G (CatG) is an endopeptidase that is associated with the early immune response. The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of CatG via a new surface plasmon resonance imaging assay. CGI-I was immobilized on the gold surface of an SPR sensor that was first modified with 1-octadecanethiol. A concentration of CGI-I equal to 4.0 μg·mL-1 and a pH of 8.0 were found to give the best results. The dynamic response of the sensor ranges from 0.25 to 1.5 ng·mL-1, and the detection limit is 0.12 ng·mL-1. The sensor was applied to detect CatG in human saliva and white blood cells

    Use of anticoagulants and antiplatelet agents in stable outpatients with coronary artery disease and atrial fibrillation. International CLARIFY registry

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    Decentralization or (re)centralization: struggle for political power in Poland

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    The author argues that in Poland the 1989 commitment to political and administrative decentralization has weakened over the last seven years and has resulted in (re)centralizing tendencies. It is argued that the center's interest in gaining short-term political successes rather than engaging in long-term reforms fostered the retention of past centralized practices and the emergence of new (re)centralizing tendencies. This has been accomplished by the central state through application of three sets of forces: (1) fiscal, (2) political, and (3) systemic (preservation of the past). The first part of the paper lays out a theoretical framework for analysis of pressures and barriers to decentralization and (re)centralization. The second is an examination of the process of implementation in Poland, of local government and public administration reform over the period 1989 - 96. The third is an examination of (re)centralization processes in the context of political and fiscal forces as well as the inability of the center to reform itself and terminate the legacy of the past system in its multidimensional form (legal, institutional, territorial). The author concludes by asserting that, although clear (re)centralizing tendencies can be identified, Polish (re)centralization is characterized by the duality of the struggle: to move forward with the reforms and to dismantle the remnants of the past centralized system. Therefore, although Polish (re)centralization may appear similar in form, it differs in terms of causes from countries that are not subject to political and economic restructuring.

    A method for determination of tocopherols in the serum

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    Chromium content in daily food rations in school canteens

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    SOCIALISM AND ITS IMPACT ON URBAN PROCESSES AND THE CITY

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