Visions beyond reality : Keat's poetic rejection of enlightenment philosophy

John Keats, as a principal figure in the Romantic movement in Europe, reacted against the philosophy of the Enlightenment. His poetry and letters are testament to his distrust of, and resistance to, an ever increasingly clinical and "reasonable" society. Keats's poetic thought--imaginative, speculative, dialectic--is the antithesis of an Enlightenment philosophy which put its faith in science, objectivity, and logical deduction. The purpose of this thesis is to examine the letters and poems of Keats through the lens of Enlightenment philosophy; it will gauge Keats's theories of the imagination in terms of that philosophy. As I move through Keats's literary response to the enlightenment, I am also examining Keats's professional progression from surgeon's apprentice--a trade strongly associated with the Enlightenment--to a primary figure in the movement away from Enlightenment and toward Romantic philosophy. This study focuses on the writings of Keats, both his letters and poetry, contextualizing his poetic thought and work while answering the critical question: How does Keats's writing reveal his reaction to Enlightenment philosophy

Both Constitutive and Infection‐Responsive Secondary Metabolites Linked to Resistance against Austropuccinia psidii (Myrtle Rust) in Melaleuca quinquenervia

Austropuccinia psidii is a fungal plant pathogen that infects species within the Myrtaceae, causing the disease myrtle rust. Myrtle rust is causing declines in populations within natural and managed ecosystems and is expected to result in species extinctions. Despite this, variation in response to A. psidii exist within some species, from complete susceptibility to resistance that prevents or limits infection by the pathogen. Untargeted metabolomics using Ultra Performance Liquid Chromatography with Ion Mobility followed by analysis using MetaboAnalyst 3.0, was used to ex-plore the chemical defence profiles of resistant, hypersensitive and susceptible phenotypes within Melaleuca quinquenervia during the early stages of A. psidii infection. We were able to identify three separate pools of secondary metabolites: (i) metabolites classified structurally as flavonoids that were naturally higher in the leaves of resistant individuals prior to infection, (ii) organoheterocyclic and carbohydrate‐related metabolites that varied with the level of host resistance post‐infection, and (iii) metabolites from the terpenoid pathways that were responsive to disease progression re-gardless of resistance phenotype suggesting that these play a minimal role in disease resistance during the early stages of colonization of this species. Based on the classes of these secondary me-tabolites, our results provide an improved understanding of key pathways that could be linked more generally to rust resistance with particular application within Melaleuca. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Transition from a maternal to external nitrogen source in maize seedlings

Maximizing NO3− uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO3− uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO3− uptake and metabolism, to gain an understanding of how the NO3− uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seed‐derived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilizing eight days after imbibition (DAI). Similarly, shoot N% decreases, but does not stabilize until 12–13 DAI. Following the decrease in free amino acid concentrations, root NO3− uptake capacity increases until shoot N% stabilizes. The increase in root NO3− uptake capacity corresponds with a rapid rise in transcript levels of putative NO3− transporters, ZmNRT2.1 and ZmNRT2.2 . The processes underlying the increase in root NO3− uptake capacity to meet N demand provide an insight into the processes controlling N uptake

Feasibility of rigid 3D image registration of high-resolution peripheral quantitative computed tomography images of healing distal radius fractures

For accurate analysis of bone formation and resorption during fracture healing, correct registration of follow-up onto baseline image is required. A per-fragment approach could improve alignment compared to standard registration based on the whole fractured region. In this exploratory study, we tested the effect of fragment size and displacement on a per-fragment registration, and compared the results of this per-fragment registration to the results of the standard registration in two stable fractures and one unstable fracture. To test the effect of fragment size and displacement, high-resolution peripheral quantitative computed tomography (HR-pQCT) scans of three unfractured radii were divided into subvolumes. Different displacements in x-, y, or z-direction or rotations around each axis were applied, and each subvolume was registered onto the initial volume to realign it. Next, registration of follow-up onto baseline scan was performed in two stable and one unstable fracture. After coarsely aligning the follow-up onto the baseline scan, a more accurate registration was performed of the whole fracture, i.e. the standard registration, and of each fracture fragment separately, i.e. per-fragment registration. Alignment was checked using overlay images showing baseline, follow-up and overlap between these scans, and by comparing correlation coefficients between the standard and per-fragment registration. Generally, subvolumes as small as 300 mm3 that were displaced up to 0.82 mm in x- or y-, or up to 1.64 mm in z-direction could be realigned correctly. For the fragments of all fractures, correlation coefficients were higher after per-fragment registration compared to standard registration. Most improvement was found in the unstable fracture and one fragment of the unstable fracture did not align correctly. This exploratory study showed that image registration of individual subvolumes, such as fracture fragments, is feasible in both stable and unstable fractures, and leads to better alignment of these fragments compared to an approach that is based on registration using the whole fractured region. This result is promising for additional analysis of bone formation and resorption in HR-pQCT studies on fracture healing

Survival efficacy of the PEGylated G-CSFs Maxy-G34 and neulasta in a mouse model of lethal H-ARS, and residual bone marrow damage in treated survivors

In an effort to expand the worldwide pool of available medical countermeasures (MCM) against radiation, the PEGylated G-CSF (PEG-G-CSF) molecules Neulasta and Maxy-G34, a novel PEG-G-CSF designed for increased half-life and enhanced activity compared to Neulasta, were examined in a murine model of the Hematopoietic Syndrome of the Acute Radiation Syndrome (H-ARS), along with the lead MCM for licensure and stockpiling, G-CSF. Both PEG-G-CSFs were shown to retain significant survival efficacy when administered as a single dose 24 h post-exposure, compared to the 16 daily doses of G-CSF required for survival efficacy. Furthermore, 0.1 mg kg of either PEG-G-CSF affected survival of lethally-irradiated mice that was similar to a 10-fold higher dose. The one dose/low dose administration schedules are attractive attributes of radiation MCM given the logistical challenges of medical care in a mass casualty event. Maxy-G34-treated mice that survived H-ARS were examined for residual bone marrow damage (RBMD) up to 9 mo post-exposure. Despite differences in Sca-1 expression and cell cycle position in some hematopoietic progenitor phenotypes, Maxy-G34-treated mice exhibited the same degree of hematopoietic stem cell (HSC) insufficiency as vehicle-treated H-ARS survivors in competitive transplantation assays of 150 purified Sca-1+cKit+lin-CD150+cells. These data suggest that Maxy-G34, at the dose, schedule, and time frame examined, did not mitigate RBMD but significantly increased survival from H-ARS at one-tenth the dose previously tested, providing strong support for advanced development of Maxy-G34, as well as Neulasta, as MCM against radiation

PEGylated G-CSF (BBT-015), GM-CSF (BBT-007), and IL-11 (BBT-059) analogs enhance survival and hematopoietic cell recovery in a mouse model of the hematopoietic syndrome of the acute radiation syndrome

Hematopoietic growth factors (HGF) are recommended therapy for high dose radiation exposure, but unfavorable administration schedules requiring early and repeat dosing limit the logistical ease with which they can be used. In this report, using a previously described murine model of H-ARS, survival efficacy and effect on hematopoietic recovery of unique PEGylated HGF were investigated. The PEGylated-HGFs possess longer half-lives and more potent hematopoietic properties than corresponding non-PEGylated-HGFs. C57BL/6 mice underwent single dose lethal irradiation (7.76-8.72 Gy, Cs, 0.62-1.02 Gy min) and were treated with various dosing regimens of 0.1, 0.3, and 1.0 mg kg of analogs of human PEG-G-CSF, murine PEG-GM-CSF, or human PEG-IL-11. Mice were administered one of the HGF analogs at 24-28 h post irradiation, and in some studies, additional doses given every other day (beginning with the 24-28 h dose) for a total of three or nine doses. Thirty-day (30 d) survival was significantly increased with only one dose of 0.3 mg kg of PEG-G-CSF and PEG-IL-11 or three doses of 0.3 mg kg of PEG-GM-CSF (p ≤ 0.006). Enhanced survival correlated with consistently and significantly enhanced WBC, NE, RBC, and PLT recovery for PEG-G- and PEG-GM-CSF, and enhanced RBC and PLT recovery for PEG-IL-11 (p ≤ 0.05). Longer administration schedules or higher doses did not provide a significant additional survival benefit over the shorter, lower dose, schedules. These data demonstrate the efficacy of BBT's PEG-HGF to provide significantly increased survival with fewer injections and lower drug doses, which may have significant economic and logistical value in the aftermath of a radiation event

VP2 potentiates the proteccion induced by VP6 against the rotavirus infection in a DNA vaccine model

Viruses like particles (VLPs) composed of VP2/VP6 are very effective in inducing protection against the rotavirus infection in animal models. Individually, VP6 also can induce protection against the infection; however, there is no information about the immunogenicity of VP2. The aim of this work was to evaluate the efficacy of DNA vaccines that codify for VP2 and VP6 alone or combined to induce protection against the rotavirus infection. Murine rotavirus VP2 and VP6 genes were cloned into the pCDNA-3 vector. Adult BALB/c mice were inoculated 3 times by intramuscular injections with 100 or 200 mg of pCDNA-3VP2 and pCDNA-3VP6, alone or combined. Two weeks after the last inoculation, mice were challenged with the murine rotavirus EDIM. We found that both plasmids pCDNA-3VP2 and pCDNA-3VP6 were able to induce rotavirus-specific serum antibodies, but not intestinal rotavirus-specific IgA. Only pCDNA-3VP6 at 200 mg could induce 30 % protection against the infection. Co-administration of 100 mg of pCDNA-3VP2 with 100 mg of pCDNA-3VP6 induced 35 % protection. When different ratios of pCDNA-3VP2/pCDNA-3VP6 were used, it was found that the co-administration of 10 µg pCDNA-3VP2/ 100 µg pCDNA-3VP6 gave the best result with up to 55 % protection. These results indicate that the DNA plasmid expressing VP6 is a better vaccine candidate that the one expressing VP2 but co-administration of both plasmids is a good alternative to potentiate the protection induced by VP6, probably by the formation of VLPs VP2/VP6 in vivo