29 research outputs found
The efficacy of hyperimmune serum in the treatment of sweating sickness
Natural and experimental cases of sweating sickness were treated using a hyperimmune serum as specific treatment and hyperimmune serum combined with symptomatic and supportive treatment based on the clinico-pathological changes observed in cases of sweating sickness. The treatment regimens were found to be highly effective in pigs and sheep as well as in calves, although recovery in the latter species was slower.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
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The presence of endotoxin activity in cases of experimentally-induced heartwater in sheep
The presence of endotoxin was examined in 5 sheep with experimentally-induced heartwater. Two peaks in endotoxin levels were recorded in 4 out of the 5 sheep during the acute stage of the disease. The 1st peak coincided with or occurred shortly after the febrile reaction (over 40 °C). The 2nd peak occurred 3-5 days after the 1st, and in 2 sheep this 2nd elevation in endotoxin levels was associated with severe clinical signs (rapid and laboured breathing, cyanosis and recumbency), and 1 of the 2 sheep died on the day of the 2nd elevation. Both endotoxin peaks were of short duration and levels had decreased in the 24-h follow-up samples.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201
A Research-Based Curriculum for Teaching the Photoelectric Effect
Physics faculty consider the photoelectric effect important, but many
erroneously believe it is easy for students to understand. We have developed
curriculum on this topic including an interactive computer simulation,
interactive lectures with peer instruction, and conceptual and mathematical
homework problems. Our curriculum addresses established student difficulties
and is designed to achieve two learning goals, for students to be able to (1)
correctly predict the results of photoelectric effect experiments, and (2)
describe how these results lead to the photon model of light. We designed two
exam questions to test these learning goals. Our instruction leads to better
student mastery of the first goal than either traditional instruction or
previous reformed instruction, with approximately 85% of students correctly
predicting the results of changes to the experimental conditions. On the
question designed to test the second goal, most students are able to correctly
state both the observations made in the photoelectric effect experiment and the
inferences that can be made from these observations, but are less successful in
drawing a clear logical connection between the observations and inferences.
This is likely a symptom of a more general lack of the reasoning skills to
logically draw inferences from observations.Comment: submitted to American Journal of Physic
The presence of Cowdria ruminantium antigen in various tissues of Amblyomma hebraeum imagines as detected by an enzyme-linked immunosorbent assay
Investigation into the presence of C. ruminantium antigen, using an enzyme-linked immunosorbent assay
(ELISA) in various tick tissues and haemolymph of adult Amblyomma hebraeum ticks revealed that the organism
invades a number of body parts and can be demonstrated in A. hebraeum. In females, the gut, salivary glands,
hypodermis and synganglion and in males, the salivary glands and gut showed the highest concentration.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201
The detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum by an enzyme-linked immunosorbent assay
A sensitive and reliable enzyme-linked immunosorbent assay for the detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum nymphae is described. For the screening of antibodies, C. ruminantium from A. hebraeum nymphae, partially purified by wheat-germ lectin affinity chromatography, was used as antigen. To screen nymph populations, sera from either Ball 3 strain-infected sheep or Kumm-strain infected mice were used. By using appropriate controls the assays were rendered specific with respect to C. ruminantium.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201
Isolation of Cowdria ruminantium by cellular affinity chromatography and detection by an enzyme-linked immunosorbent assay
The isolation of Cowdria ruminantium by means of wheat germ lectin affinity chromatography as described
in this paper permits the recovery of partially purified viable organisms under mild conditions in short time.
These conclusions are based upon results of analyses of column fractions by intravenous inoculation into sheep,
protein determination, electronmicroscopy and enzyme-linked immunosorbent assay. The entire purification
procedure could be completed in 4-5 hours using only either infected sheep tissue or nymphae as starting
material.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
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The clinical pathology of heartwater (I): hematology and blood chemistry
Clinical pathological studies were undertaken in 5 calves with experimentally-induced heartwater. The most important findings include a progressive anaemia which may be associated with bone marrow depression and fluctuations in the total and differential white cell count, of which an eosinopenia and a lymphocytosis were the most marked. A severe drop in serum protein, especially in the albumin levels, was observed in all 5 cases. This disease is probably associated with an increased capillary permeability, as the protein content of the pericardial fluid in 1 case that died, approximated that of the serum. The osmolality of the effused fluid was also higher than that of the blood. No significant changes in the serum electrolyte levels occurred, except for total calcium levels which tended to decrease to below normal during the acute stage of the disease. Marked increases in total bilirubin were recorded. This, however, was not associated with liver pathology or haemolysis and may possibly be ascribed to a fasting hyperbilirubinaemia. Darkening of plasma colour was associated with peak rises in total bilirubin. Increases in both blood urea and creatinine levels indicate interference with renal glomerular filtration during the acute stage of the disease
Heartwater : past, present and future : proceedings of a workshop held at Berg en Dal, Kruger National Park, on 8-16 September 1986
The events leading to the production of an effective vaccine against heartwater are summarized. The production techniques used at Onderstepoort to produce and control the quality of the infected blood and nymph suspension vaccines, and covering their respective advantages and disadvantages, are compared. The many problems of these vaccines and how they may best be overcome are discussed.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201
Detection of Cowdria ruminantium antigen and antibody during the course of heartwater disease in sheep by means of an enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay was used to detect Cowdria ruminantium antibodies during the course of heartwater disease. IgM antibodies reached a maximum on the 4th day after infection and disappeared on the 7th day. IgG antibodies first appeared on the 8th day and continued to increase during the remainder of the
observation period of 28 days. The presence of C. ruminantium in the blood fractions of diseased animals was demonstrated by an enzyme-linked immunosorbent assay. The earliest day of C. ruminantium antigen detection was in plasma and serum on the 4th day after inoculatton. Of all the blood fractions investigated, the red blood cells showed the highest concentration, and this reached a maximum on the 12th day after infection.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
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Heartwater : past, present and future : proceedings of a workshop held at Berg en Dal, Kruger National Park, on 8-16 September 1986
The importance of further research on the susceptibility of wild hosts to Cowdria ruminantium infection is discussed. The literature is surveyed and an attempt is made to divide the various species described into susceptible and refractory hosts. The reasons or the numerous apparently conflicting reports are considered and it is suggested that those making further inquiries in this field of work take these factors into account.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201