Characterisation of the microbiome along the gastrointestinal tract of growing turkeys

The turkey microbiome is largely understudied, despite its relationship with bird health and growth, and the prevalence of human pathogens such as Campylobacter spp. In this study we investigated the microbiome within the small intestine (SI), caeca (C), large intestine (LI) and cloaca (CL) of turkeys at 6, 10 and 16 weeks of age. Eight turkeys were dissected within each age category and the contents of the SI, C, LI and CL were harvested. 16S rDNA based QPCR was performed on all samples and samples for the 4 locations within 3 birds/age group were sequenced using ion torrent-based sequencing of the 16S rDNA. Sequencing data showed on a genus level, an abundance of Lactobacillus, Streptococcus and Clostridium XI (38.2, 28.1 and 13.0% respectively) irrespective of location and age. The caeca exhibited the greatest microbiome diversity throughout the development of the turkey. PICRUSt data predicted an array of bacterial function, with most differences being apparent in the caeca of the turkeys as they matured. QPCR revealed that the caeca within 10 week old birds, contained the most Campylobacter spp. Understanding the microbial ecology of the turkey gastrointestinal tract is essential in terms of understanding production efficiency and in order to develop novel strategies for targeting Campylobacter spppublishersversionPeer reviewe

Russian patronage over Abkhazia and South Ossetia: implications for conflict resolution

This article applies the concepts of linkage and leverage as developed by Steven Levitsky and Lucan Way and elaborated by Gwendolyn Sasse in the introduction to this special issue of East European Politics to Russia’s involvement with Abkhazia and South Ossetia, the two quasi-independent states in the South Caucasus. Through this re-conceptualisation of the developments in Abkhazia and South Ossetia, combined with new empirical insights, the article aims to increase our understanding of the conflicts in the region, of the involvement of the Russian Federation in particular, and consequently of the prospects of international conflict resolution. Compared to other political entities in the former Soviet Union, Abkhazia and South Ossetia represent an extreme case of Russian linkage and leverage. This article focuses on the period after the Russian–Georgian war in 2008, when Russia recognised the regions’ independence and effectively turned them into Russian protectorates. The economic, intergovernmental, technocratic and social linkages between Russia and the two regions are extraordinarily deep, and they directly undermine the autonomy of the regions. The post-war delegation of Russian cadres and institutional and legislative diffusion creates additional linkages underpinning Russian leverage.History and International Relation

Multicomponent Synthesis of Diverse 1,4-Benzodiazepine Scaffolds

The 1,4-benzodiazepine (BDZ) scaffold is of particular interest in drug design due to a balanced ensemble of beneficial physicochemical properties including a semirigid and compact diazepine ring with spatial placements of several substituents, combined with low number of rotatable bonds, hydrogen bond donors and acceptors, and intermediate lipophilicity. As an alternative to traditional multistep sequential syntheses, we designed routes employing one-pot MCRs to accelerate access diverse BDZ scaffolds in two or three steps