ORAL RABIES IMMUNIZATION OF SWINE: USE OF VNUKOVO-32/107 VACCINATION STRAIN

The apathogenicity and antigenic activity of the live oral rabies vaccine prepared from the strain Vnukovo-32/107 were evaluated in two experiments carried out on non-target speciesswine (domestic swine Sus scrofa domestica and wild boar Sus scrofa). The Vnukovo-32/107 vaccination strain has been used to produce oral rabies vaccine Kamark for immunization of free living carnivores. Wild boars compete with foxes for acceptance of baits distributed in the field. The tested vaccination strain for oral application (including very high doses) proved apathogenic to non-target species -swine. Detection and quantification of rabies antibodies were carried out by an immunoenzymatic assay -ELISA, rapid fluorescence focus inhibition test (RFFIT) and virus neutralization test (VNT) on mice on days 30 and 90 post-immunization. The values obtained were expressed in international units (IU/cm 3 ); the value of 0,5 IU/cm 3 was used as a positivity criterium. Antirabic antibodies were detected in more than 50 % of animals in all three groups of two animal sets on day 30 post-immunization. The results obtained show good antigenic activity of the live oral rabies vaccine prepared from the strain Vnukovo-32/107. apathogenicity; antigenic activity Rabies, vaccination strai