Multiplication of the patato tuber moth granulosis virus in Phthorimaea operculella cell cultures

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Natural DNA-recombinations of Spodoptera littoralis NPV among field populations in Egypt

Six natural isolates belonging to #Spodotera littoralis$ NPV were identified. Using the restriction endonucleases Eco RI and Bam HI, the DNA profiles of each isolate from Giza, Menya, Kaliubeia-Kaha, Kafr El-Sheikh, Gharbeia and Kaliubeia-Tokh were differentiated from that of the reference Egypt-84. DNA electrophoretic profiles on different isolates collected 5 years later were compared to the difference type. The DNA sequence was not changed trough three serial passages of viral isolates in vivo, while several viral clones were obtained after the 4th passage. (Résumé d'auteur

Establishment of two cell lines from embryonic cells of Pectinophora gossypiella (Lepidoptera : Gelichiidae)

Two cell lines from the most important insect pest of cotton, #Pectinophora gossypiella S., were obtained from an embryonic tissue culture. The cells were cultured in GRACE's modified medium. The first cell line, designated ORS-PgS (PgS), was obtained by selecting the non-adherent cells during subculturing and consisted of a homogeneous population of rounded cells. The second cell line, designated ORS-PgA (PgA), obtained without any artificial selection, was a heterogeneous population of spherical and spindle-shaped cells with a great capacity to adhere. The two cell lines had a doubling time of 40 and 48 h respectively and were sub-cultured for more than 50 passages. Their polypeptidic profiles appeared identical but were different from those of other lepidopteran cell lines tested. The two cell lines support the multiplication of #Autographa california nucleopolyhedrovirus, producing 100000 plaque forming units/ml. In 1 to 2% PgS cells, polyhedra were synthetized, whereas PgA cells multiplied only virions. (Résumé d'auteur

Establishment of a cell line derived from embryos of the potato tuber moth Phthorimaea operculella (Zeller)

Une lignée cellulaire a été obtenue à partir d'embryocultures de #Phthorimaea operculella (Zeller), le principal ravageur de la pomme de terre dans les régions tropicales et sub-tropicales. La lignée cellulaire, nommée ORS-Pop-93, cultivée en milieu Grace modifié, a une population hétérogène de cellules arrondies et allongées très adhérentes. Le temps de doublement des cellules est de 40 h. La lignée a été subcultivée plus de 40 fois. Le profil polypeptidique est différent de celui d'autres lignées de lépidoptères. La lignée est permissive à la polyhédrose nucléaire de #Autographa californica. (Résumé d'auteur

Mise en évidence d'un virus de granulose chez Sesamia cretica Led. (Lepidoptera Noctuidae), principal ravageur du maïs d'Afrique du Nord-Est : caractérisation de l'ADN génomique et diagnostic viral

Un virus de granulose a été isolé du foreur de tige de maïs #Sesamia cretica Led. (Lepidoptera, Noctuidae) en Egypte. L'ADN de ce Baculovirus, dénommé ScGV (#S. cretica Granulosis Virus), a été caractérisé. La taille du génome a été estimée à 112 kilobases et des profils caractéristiques de restriciton enzymatique ont été dressés pour 17 endonucléases. Des méthodes de diagnostic viral, test immunoenzymatique ELISA et sonde nucléique totale, ont été établies et testées en vue d'études épidémiologiques de la maladie. (Résumé d'auteur