Non-resonant background suppression in preresonance CARS spectra of flavin adenine dinucleotide: Demonstration of a background suppression technique using phase mismatching and comparison with the polarization-sensitive CARS technique

Polarization-sensitive CARS spectra of a 5.7 × 10-3 mol dm-3 flavin adenine dinucleotide (FAD) solution were recorded under preresonance conditions at a pump wavelength of 532 nm. The depolarization ratios of the vibrations are shown to be close to the depolarization ratio of the non-resonant background. This results in a severe reduction of the vibration resonant signal (a factor of 700-900) in the polarization CARS spectrum, and a poor improvement in the ratio of the resonant signal and the non-resonant background (<10). \ud In this context, a non-resonant background suppression technique is discussed and demonstrated for 5.7 × 10-3 and 1.4 × 10-3 mol dm-3 FAD solutions excited at 532 nm; the non-resonant susceptibility of the walls of the cuvette, which contains the FAD solution, is used to compensate the non-resonant signal contribution of the solution. An improvement in the signal-to-noise ratio of ca. 50 is achieved at the cost of a factor of 30 in the resonant signal strength. Lorentzian-shaped spectral bands are obtained, facilitating the determination of band position, width and intensity. Line shape parameters and depolarization ratios for FAD are extracted from the presented spectra by curve fitting. The signal strength and background suppression achieved with these techniques and the resonance CARS technique (at a pump wavelength of 480 nm) are compared and discussed

Auditory and visual capture during focused visual attention

It is well known that auditory and visual onsets presented at a particular location can capture a person’s visual attention. However, the question of whether such attentional capture disappears when attention is focused endogenously beforehand has not yet been answered. Moreover, previous studies have not differentiated between capture by onsets presented at a nontarget (invalid) location and possible performance benefits occurring when the target location is (validly) cued. In this study, the authors modulated the degree of attentional focus by presenting endogenous cues with varying reliability and by displaying placeholders indicating the precise areas where the target stimuli could occur. By using not only valid and invalid exogenous cues but also neutral cues that provide temporal but no spatial information, they found performance benefits as well as costs when attention is not strongly focused. The benefits disappear when the attentional focus is increased. These results indicate that there is bottom-up capture of visual attention by irrelevant auditory and visual stimuli that cannot be suppressed by top-down attentional control

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

The last recorded presence of the Eurasian otter (Lutra lutra) in the Netherlands dates from 1989 and concerned a dead individual. In 2002 a reintroduction programme was started, and between June 2002 and April 2008 a total of 30 individuals (10 males and 20 females) were released into a lowland peat marsh in the north of the Netherlands. Noninvasive genetic monitoring based on the genetic profiles obtained from DNA extracted from otter faeces (spraints) was chosen for the post-release monitoring of the population. To this end, the founding individuals were genotyped before release and spraints were collected in the release area each winter from 2002 to 2008. From June 2002 to April 2008 we analysed the genetic profile of 1,265 spraints on the basis of 7–15 microsatellite loci, 582 of which (46%) were successfully assigned to either released or newly identified genotypes. We identified 54 offspring (23 females and 31 males): the females started to reproduce after 2 years and the males after 4 years. The mating and reproductive success among males was strongly skewed, with a few dominant males fathering two-thirds of the offspring, but the females had a more even distribution. The effective population size (Ne) was only about 30% of the observed density (N), mainly because of the large variance in reproductive success among males. Most juvenile males dispersed to surrounding areas on maturity, whereas juvenile females stayed inside the area next to the mother’s territory. The main cause of mortality was traffic accidents. Males had a higher mortality rate (22 out of 41 males (54%) vs. 9 out of 43 females (21%)). During winter 2007/08 we identified 47 individuals, 41 of which originated from mating within the release area. This study demonstrates that noninvasive molecular methods can be used efficiently in post-release monitoring studies of elusive species to reveal a comprehensive picture of the state of the populatio

Equilibrium-Independent Control of Continuous-Time Nonlinear Systems via the LPV Framework -- Extended Version

In this paper, we consider the analysis and control of continuous-time nonlinear systems to ensure universal shifted stability and performance, i.e., stability and performance w.r.t. each forced equilibrium point of the system. This "equilibrium-free" concept is especially beneficial for control problems that require the tracking of setpoints and rejection of persistent disturbances, such as input loads. In this paper, we show how the velocity form, i.e., the time-differentiated dynamics of the system, plays a crucial role in characterizing these properties and how the analysis of it can be solved by the application of Linear Parameter-Varying (LPV) methods in a computationally efficient manner. Furthermore, by leveraging the properties of the velocity form and the LPV framework, a novel controller synthesis method is presented which ensures closed-loop universal shifted stability and performance. The proposed controller design is verified in a simulation study and also experimentally on a real system. Additionally, we compare the proposed method to a standard LPV control design, demonstrating the improved stability and performance guarantees of the new approach.Comment: Non-extended version submitted to IEEE Transactions on Automatic Contro

Convex Equilibrium-Free Stability and Performance Analysis of Discrete-Time Nonlinear Systems

This paper considers the equilibrium-free stability and performance analysis of discrete-time nonlinear systems. We consider two types of equilibrium-free notions. Namely, the universal shifted concept, which considers stability and performance w.r.t. all equilibrium points of the system, and the incremental concept, which considers stability and performance between trajectories of the system. In this paper, we show how universal shifted stability and performance of discrete-time systems can be analyzed by making use of the time-difference dynamics. Moreover, we extend the existing results for incremental dissipativity for discrete-time systems based on dissipativity analysis of the differential dynamics to more general state-dependent storage functions for less conservative results. Finally, we show how both these equilibrium-free notions can be cast as a convex analysis problem by making use of the linear parameter-varying framework, which is also demonstrated by means of an example.Comment: Submitted to IET Control Theory & Application