62 research outputs found
Purification and characterization of a novel neutral and heat-tolerant phytase from a newly isolated strain Bacillus nealsoniiZJ0702
Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis
Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum
Analysis of myo-inositol hexakisphosphate hydrolysis by Bacillus phytase:Indication of a novel reaction mechanism
Phytic acid (myo-inositol hexakisphosphate, InsP(6)) hydrolysis by Bacillus phytase (PhyC) was studied. The enzyme hydrolyses only three phosphates from phytic acid. Moreover, the enzyme seems to prefer the hydrolysis of every second phosphate over that of adjacent ones. Furthermore, it is very likely that the enzyme has two alternative pathways for the hydrolysis of phytic acid, resulting in two different myo-inositol trisphosphate end products: Ins(2,4,6)P(3) and Ins(1,3,5)P(3). These results, together with inhibition studies with fluoride, vanadate, substrate and a substrate analogue, indicate a reaction mechanism different from that of other phytases. By combining the data presented in this study with (1) structural information obtained from the crystal structure of Bacillus amyloliquefaciens phytase [Ha, Oh, Shin, Kim, Oh, Kim, Choi and Oh (2000) Nat. Struct. Biol. 7, 147-153], and (2) computer-modelling analyses of enzyme-substrate complexes, a novel mode of phytic acid hydrolysis is proposed
Identification of two novel bacterial phosphataseâencoding genes in Pseudomonas putida
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