Antioksidativna aktivnost metanolskog ekstrakta lista biljke Saxifraga Rotundifolia L.

The aim of this study was to investigate the potential antioxidant activity of the methanol leaf extract of Saxifraga rotundifolia L., depending on the total quantity of phenols, flavonoids and flavonol. The tested extracts showed high phenolic content (324.39 mg GA/g) and small amounts of flavonoids (36.81 mg RU/g) and flavonols (11.52 mg RU/g). The antioxidant activity was determined by a series of in vitro methods. The tested extracts showed high level of total antioxidant activity (509.78 mg AA/g), and high degree of ABTS+ and DPPH "scavenger" activity with IC50 value of 48.76 μg/mL for ABTS+ method and 30.39 μg/mL for DPPH method. The results indicate that the tested extract has significant antioxidant activity and can be a source of natural antioxidants.Cilj rada je bio da se ispita potencijalna antioksidativna aktivnost metanolskog ekstrakta lista biljke Saxifraga rotundifolia L. u zavisnosti od ukupne količine fenola, flavonoida i flavonola. Ispitivani ekstrakt pokazao je visok sadržaj fenola (324,39 mg GA/g) i manje količine flavonoida (36,81 mg RU/g) i flavonola (11,52 mg RU/g). Antioksidativna aktivnost određena je serijom in vitro metoda. Ispitivani ekstrakt pokazuje visok nivo ukupne antioksidativne aktivnosti (509,78 mg AA/g), kao i visok stepen АBTS+. i DPPH ,,skevindžer“ aktivnosti sa IC50 vrednošću od 48,76 μg/mL za ABTS+. i 30,39 μg/mL za DPPH metodu. Dobijeni rezultati ukazuju da ispitivani ekstrakt poseduje značajnu antioksidativnu aktivnost i može biti izvor prirodnih antioksidanata

Alchemilla Vulgaris L. kao potencijalni izvor prirodnih antioksidanata

The aim of this study was to examine the total phenolic and flavonoid content, and antioxidant activity of the methanol extract of Alchemilla vulgaris aerial parts, as a potential source of natural antioxidants. The obtained results showed that extract has high total phenolic content (590,22 mg GAE/g), wheras flavonoid content was lower (12,58 mg RU/g). Antioxidant activity was determined in vitro by DPPH· and ABTS+· reagents. The IC50 values of DPPH (5,40 μg/ml) and ABTS+· scavenging capacities (60,10 μg/ml) of methanol extract showed that tested extract has extremely strong antioxidant activity.Cilj ovog rada je bio ispitivanje antioksidativne aktivnosti, kao i količine ukupnih fenolnih jedinjenja i flavonoida u metanolnom ekstraktu biljke A. vulgaris L., kao potencijalnog izvora prirodnih antioksidanata. Ispitivani ekstrakt poseduje visok sadržaj ukupnih fenola (590,22 mg GA/g), dok je koncentracija flavonoida znatno manja (12,58 mg RU/g). Antioksidativna aktivnost je ispitivana in vitro DPPH i ABTS+· metodom, a IC50 vrednosti ukazuju da ispitivani ekstrakt pokazuje izuzetno visok stepen antioksidativne aktivnosti u poređenju sa referentnim fenolnim jedinjenjima, što ukazuje na mogućnost primene ekstrakta A. vulgaris u prehrambenoj industriji

Stabilnost ekstrakta korena suručice i efekat na sprečavanje lipidne oksidacije u mesu

Nowadays, consumers are demanding more natural foods, obliging the industry to include natural antioxidants in foods and synthetic antioxidants have been rejected because of their carcinogenicity. Natural antioxidants have been used instead of synthetic to retard lipid oxidation in foods to improve their quality and nutritional value. The aim of this work was to evaluate pH and termal stability, and effect on lipid oxidation in meat-model system of methanolic extract of Filipendula hexapetala Gilib. Results suggest that the stability of the extract is expressed in a variety of pH and thermal conditions. The root extract of F. hexapetala exerted a concentration-dependent antioxidant activity in a meat model system, lower than synthetic antioxidant BHT.Danas je u ishrani zastupljen veliki broj prirodnih proizvoda, tako da prehrambena industrija sve više koristi prirodne antioksidante, a sintetički antioksidanti se izbacuju iz upotrebe zbog njihove kancerogenosti. Prirodni antioksidanti se koriste umesto sintetičkih da uspore oksidaciju lipida, poboljšaju kvalitet i nutritivnu vrednost namirnica. Cilj ovog rada bio je određivanje pH i termalne stabilnosti, kao i efekta na lipidnu oksidaciju u mesu metanolskog ekstrakta korena biljke Filipendula hexapetala Gilib. Rezultati ukazuju da je stabilnost ekstrakta izražena u različitim pH i termalnim uslovima. Ekstrakt korena F. hexapetala pokazao je koncentraciono-zavisnu antioksidativnu aktivnost u meso model-sistemu, nešto nižu u odnosu na sintetički antioksidant BHT

FORMATION AND SINTERING OF γ-Al2 O3 WITH MgO ADDITIONS FROM COPRECIPITATED HYDRATES

Des poudres d'alumine-γ avec un dopage homogène ont été obtenues par calcination des coprécipités A100H - Mg(OH)2 formant des solutions solides. L'influence du dopage, pour des rapports Mg/Al de 0.02 à 0.06 sur le frittage a été analysée par mesure de la densité, de la porosité, des retraits linéaires ainsi que par détermination de la résistance à la traction.The gamma Al2O3 powders with homogeneous doppant distribution were obtained by calcination of coprecipitates consisted of A100H solid solutions with Mg(OH)2. The effect of additive with Mg/Al ion ratio 0.02-0.06 on sintering was analysed by density, porosity, linear shrinkage and tensile strength determinations

Lythrum salicaria L. (Lythraceae) as a promising source of phenolic compounds in the modulation of oxidative stress: Comparison between aerial parts and root extracts

Lythrum salicaria is a flowering plant traditionally used for inflammatory diseases, gastrointestinal ailments, dysentery, and as astringent for external use. The aim of this study was to determine the phenolic content, antioxidant, cytotoxic, and antimicrobial activities of L. salicaria aerial part and root methanol extracts, as well as the protective effects against oxidative damage on DNA and on a cell-based model. Moreover, simulated in vitro digestion studies were performed to estimate the stability of individual compounds present in the extracts. L. salicaria extracts contained a significant amount of total phenolic compounds and several phenolic compounds including C-glycoside flavones, orientin, isoorientin, and vitexin, were identified in extracts. The extracts demonstrated high antioxidant potential in different in vitro methods, moderate antimicrobial properties, and low cytotoxicity on studied normal and cancer cell lines. Also, the extracts showed significant DNA protection and protection of cells against arsenite-induced oxidative stress. These results suggest that, although the aerial part of L. salicaria is in extensive use since ancient times as a medicinal plant, its root may also possess the valuable biological properties and both could be used as functional food ingredients, considering their good antioxidant and antigenotoxic activities, and stability during in vitro digestion