On-chip synthesis of fine-tuned bone-seeking hybrid nanoparticles

Aims: Here we report a one-step approach for reproducible synthesis of finely tuned targeting multifunctional hybrid nanoparticles (HNPs). Materials & methods: A microfluidic-assisted method was employed for controlled nanoprecipitation of bisphosphonate-conjugated poly(D,L-lactide-co-glycolide) chains, while coencapsulating superparamagnetic iron oxide nanoparticles and the anticancer drug Paclitaxel. Results: Smaller and more compact HNPs with narrower size distribution and higher drug loading were obtained at microfluidic rapid mixing regimen compared with the conventional bulk method. The HNPs were shown to have a strong affinity for hydroxyapatite, as demonstrated in vitro bone-binding assay, which was further supported by molecular dynamics simulation results. In vivo proof of concept study verified the prolonged circulation of targeted microfluidic HNPs. Biodistribution as well as noninvasive bioimaging experiments showed high tumor localization and suppression of targeted HNPs to the bone metastatic tumor. Conclusion: The hybrid bone-targeting nanoparticles with adjustable characteristics can be considered as promising nanoplatforms for various theragnostic applications

Microfluidic-Assisted Self-Assembly of Complex Dendritic Polyethylene Drug Delivery Nanocapsules

Microfluidic platform for the synthesis of complex nanocapsules is presented via a controlled self-assembly. The monodisperse nanocapsules in the range of 50–200 nm consist of a dendritic polyethylene core and a Pluronic copolymer shell. The resultant nanocarriers encapsulate large amount of hydrophobic anticancer drug like paclitaxel while providing a low complement activation as well as sustained release profile with high tunability

Data Available Statement, manuscript: Cell survival and differentiation with nanocrystalline glass-like carbon using substantia nigra dopaminergic cells derived from transgenic mouse embryos. Rodriguez-Losada et al

This file makes data available in a public repository from the PlosOne article titled: Cell survival and differentiation with nanocrystalline glass-like carbon using substantia nigra dopaminergic cells derived from transgenic mouse embryos. Rodriguez-Losada, N et al. 20171. Funds from the University of Malaga and Campus de Excelencia Internacional Andalucía Tech (UMA) 2. Junta de Andalucia Government, Spain (PAIDI-CTS156) 3. Spanish Ministry of Science and Innovation through the Ramon y Cajal Fellowship (RG