RNA Synthesis in the Tracheal Epithelial Cells of Aging Mice as Revealed by Electron Microscopic Radioautography

For the purpose of studying the aging changes of macromolecular synthesis in the tracheal epithelial cells of experimental animals, we studied 10 groups of aging mice during development and aging from fetal day 19 to postnatal month 24. They were injected with 3H-uridine, a precursor for RNA synthesis, sacrificed and the tracheal tissues were taken out, fixed and processed for light and electron microscopic radioautography. On many radioautograms the localization of silver grains demonstrating RNA synthesis in tracheal epithelial cells in respective aging groups were analyzed qualitatively and quantitatively. The number of mitochondria per cell, the number of labeled mitochondria with silver grains and the number of silver grains in each cell in respective aging groups were analyzed quantitatively in relation to the aging of animals. The results revealed that the RNA synthetic activity as expressed by the incorporations of RNA precursor, i.e., the number of silver grains in cell nuclei, cell organelles, changed due to the aging of animals. The number of mitochondria, the number of labeled mitochondria and the mitochondrial labeling index labeled with silver grains were counted in each tracheal epithelial cell. It was demonstrated that the number of mitochondria increased from embryonic day 19 to postnatal newborn day 1, 3, 9, 14, adult month 1, 2, reaching the maximum and kept plateau, while the number of labeled mitochondria increased from embryonic day 19 to postnatal adult month 2, reaching the maximum but the labeling indices showing RNA synthesis increased from embryonic day 19 to postnatal newborn day 14 and adult month 1 and decreased to year 2, indicating the aging changes. Based upon our findings, available literature on macromolecular synthesis in mitochondria of various cells are reviewed

Cholecystitis & An Enzyme Study

Cholecystitis is inflammation of the gallbladder that develops in short time usually when gallstone obstructs the cystic duct. Patients over the passage of time land to chronic cholecystitis. They have an abnormal liver function test with clinical features suggestive of gall bladder disease. Therefore, systematic step by step reviews of various investigations are important in diagnosis of gall bladder disease. The first step includes clinical evaluation of the patient followed by estimation of enzyme markers.. The seriousness of disease can be estimated from combined information of clinical examination & specialized biochemical tests. Specialized enzymatic markers are helpful for proper follow-up as delay can be devastating. It can form a platform for malignant & cirrhotic changes of liver: Present study has been undertaken to avoid dreads by simple clinical enzyme study. Serum levels of 5’NT/ALP/AST/ALT/Bilirubin were estimated in sixty cases of clinically diagnosed cholecystitis against forty normal individuals. Purpose was to single out a parameter which is most significant & may help as an endoscope to \ud Surgeon for timely intervention. The study delineates5’NT to be superior to ALP due to its specificity &. Sensitivity. While elevated AST & ALT levels signify extent of hepatic cell damage, 5”NT specifically signifies the bile duct obstruction or cholestasis as well as hepatic cell damage. \u

Tamsulosin � turn a round

Tamsulosin is a sulfamoylphen-ethylamine derivative, a potent and a selective antagonist of Alpha-1A adrenoceptor. It�s approved in the treatment of LUTS in BPH disease, being a specific Alpha -1A blocker it does not interfere much with the cardiovascular system. Though an age old molecule but still it�s a friendly drug to most of the physicians. Even the recent studies found its as efficacious to some of the newer molecules in the group

Viral Hepatitis And Enzyme Study

The present study was undertaken in sixty patients of viral hepatitis of both sexes ranging 20 to 60 yrs. of age to compare serum levels or 5’Nucleotidase, Alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase & serum bilirubin. Forty of clinically healthy subjects were taken as control group. These were patient’s attendants without any evidence of liver disease so as to equlibriate the socioeconomic status and age. The study group patients were either admitted to Rajindra Hospital, Patiala, or attending the OPD. A detailed clinical examination was carried out in all as per plan mentioned in materials & methods. Diagnosis of these patients was based on clinical findings. Endeavour was to find out a single enzyme marker which is most specific and sensitive parameter out of all above. It was later concluded that value of 5’ NT was highly significant as compared to ALP. A positive correlation was observed between AST, ALT & serum bilirubin level. But 5’ NT showed no correlation with aminotransferases

Studies on association of arbuscular mycorrhizal fungi with gluconacetobacter diazotrophicus and its effect on improvement of sorghum bicolor (L.)

Considerable attention has been paid on endophytic diazotrophs in recent times, because of its of ability to fix and transfer fixed nitrogen to the host plant. Arbuscular mycorrhizal (AM) fungi are ubiquitous and play a significant role in improving the growth of plants through better uptake of nutrients, especially phosphorus. Interaction between AM fungi and nitrogen fixing bacteria and its impact on the host plant has been studied in several instances. In the present study, an attempt has been made to know the combining ability of G.diazotrophicus with AM fungi on S.bicolor. Spores of ten species of AM fungi were isolated from the rhizosphere soils of S.bicolor from different localities of Madurai and Sivagangai districts of Tamil Nadu. G.diazotrophicus was isolated from stem tissues of sugarcane (Saccharrum officinarum L.) from Madurai districts. The AM fungi in association with G.diazotrophicus were evaluated on the basis of root colonization, fresh and drymatter yield, N, P, soluble sugars and photosynthetic pigments in leaves of S.bicolor. Fresh weight and dry weight was significantly higher in dual inoculated plants. The highest values were recorded with Glomus fasciculatum + G.diazotrophicus combination. AM fungal infection was significantly higher in dual inoculated plants. N concentration was significantly increased by G.diazotrophicus even more in association with the efficient fungal strains. Dual inoculated plants showed a significant increase in P, soluble sugars, photosynthetic pigments in leaves was observed in G.diazotrophicus + Glomus fasciculatum combination. Such morphological modification may enhance water and nutrient uptake. Our results confirm the importance of studying plant-microbial interrelationship to provide useful information for agricultural system management

Effect of obesity on autonomic nervous system

The present study was carried out on 100 volunteers of which 50 subjects with BMI > 30kg/m2 were included in study group and 50 subjects with BMI < 30kg/m2 (non obese) were included in control group. The functioning of Autonomic nervous system was evaluated by six non-invasive tests- four of which were based mainly on parasympathetic control (30:15 ratio, standing to lying ratio (S/L ratio), expiration/inspiration ratio (E/I ratio) and valsalva maneuver) and two on sympathetic control (Blood pressure response to standing and cold pressor test). The results of the present study showed significantly low (p=0.001) S/L ratio in study group (1.04 ± 0.12) when compared to controls (1.12 ± 0.11) indicating impaired parasympathetic function. The mean change in sytolic blood pressure before and after cold pressor test (CPT) was less in study group (7.12 ± 5.28) as compared to control group (10.38 ± 6.35) and this was statistically significant (p=0.006) indicating impaired sympatheitc function. Thus ,in obese both division of ANS are affected which may be the cause of various cardiovascular complications

Study of Histamine Forming Bacteria in Commercial fish samples of Kalyan city

Histamine food poisoning is found to be associated with consumption of scombroid fish containing unusually high levels of histamine. Fish belonging to non-scombroid group may also cause histamine poisoning. In this study, histamine forming bacteria in the commercial fish samples of local markets of Kalyan region were investigated. Among 54 isolates 24 were found to be prominent histamine producers. A simple and rapid colorimetric method for the quantification of histamine in fish was used. Histamine level in fresh mackerel samples was found to be around 20 mg/100 g, which was much above the defect action level (5 mg/100 g) given by FDA indicating potential risk for histamine poisoning. The study suggest that practice of more hygienic and sanitary conditions during handling and processing of fish are required to minimize the contamination of such histamine producing bacteri

A Study on Ajuga bracteosa wall ex. Benth for analgesic activity

Ethnopharmacological relevance: Ajuga bracteosa wall ex. Benth (Labiatae) is traditionally used medicine in the treatment of malaria and gout. The plant is substitute of cinchona. Its allied species Ajuga Parviflora is also found sporadically. In Ayurvedic prepration the aqueous extract of the leaves part showed diuretic activity. Aim of the study: The present study was carried out to investigate analgesic activity of Ajuga bracteosa wall ex. Benth aerial part extracts. Materials and methods: A. bracteosa, a widespread medicinal plant traditionally used in the disease, was collected from Hamirpur district of Himachal Pradesh. Aerial part was extracted with petroleum ether, chloroform, methanol, ethanol and water. Analgesic activity of these extracts was assessed in swiss albino mice with acetic acid-induced writhing test and tail immersion test. Results: At the doses used (200 and 400 mg/kg, i.p.) chloroform and water extracts showed significant and dose-dependent analgesic effects.\ud Conclusion: Our results indicate that extracts Ajuga bracteosa wall ex. Benth obtained from demonstrate an analgesic effect probably mediated by opioid receptors.\u

Multilocular Cystic Nephroma – A Surgical and Radiological Dilemma

Multilocular cystic nephroma is a slow growing benign renal tumor. It has been identified as exclusive adult lesion, more common in females. It commonly occurs as an asymtomatic mass, occasionally with hematuria. Clinical presentation & radiological features puts urologist under dilemma. Only a few studies have correlated the Bosniak renal cyst classification with pathological findings; none of them has managed to recruit many patients, and all have case selection bias. Although new imaging techniques are available, surgical excision and histological analysis of the tumor are the only effective methods to distinguish benign from malignant cystic lesions of the kidney. Here in we present a case to highlight the need for proper diagnosis and designation of these tumors. These renal tumors are benign and carry an excellent prognosis

Peristaltic flow of a Newtonian fluid through a porous medium in a vertical tube under the effect of a magnetic field

In this paper, we studied the effects of heat transfer and magnetic field with peristaltic flow of a viscous incompressible Newtonian fluid through a porous medium in a vertical tube under the assumptions of long wavelength and low Reynolds number. The closed form solutions of velocity field and temperature are obtained. The influence of various pertinent parameters on the flow characteristics, the temperature and the heat transfer coefficient are discussed through graphs