Reply to the comment on “Accuracy and usability of a diagnostic decision support system in the diagnosis of three representative rheumatic diseases: a randomized controlled trial among medical students”

With great interest, we read the comment by Gilbert and Wicks on our recent publication [1] testing the accuracy and usability of Ada’s symptom checker among medical students

Discrepancies in Determinations of the Ginzburg-Landau Parameter

Long-standing discrepancies within determinations of the Ginzburg-Landau parameter $\kappa$ from supercritical field measurements on superconducting microspheres are reexamined. The discrepancy in tin is shown to result from differing methods of analyses, whereas the discrepancy in indium is a consequence of significantly differing experimental results. The reanalyses however confirms the lower $\kappa$ determinations to within experimental uncertainties.Comment: submitted to Phys. Rev.

Full length interleukin 33 aggravates radiation-induced skin reaction

The interleukin (IL)-1 family member IL-33 has been described as intracellular alarmin with broad roles in wound healing, skin inflammation but also autoimmunity. Its dichotomy between full length (fl) IL-33 and the mature (m) form of IL-33 and its release by necrosis is still not fully understood. Here, we compare functional consequences of both forms in the skin in vivo, and therefore generated two lines of transgenic mice which selectively overexpress mmIL-33 and flmIL-33 in basal keratinocytes. Transgene mRNA was expressed at high level in skin of both lines but not in organs due to the specific K14 promoter. We could demonstrate that transgenic overexpression of mmIL-33 in murine keratinocytes leads to a spontaneous skin inflammation as opposed to flmIL-33. K14-mmIL-33 mice synthesize and secrete high amounts of mmIL-33 along with massive cutaneous manifestations, like increased epidermis and dermis thickness, infiltration of mast cells in the epidermis and dermis layers and marked hyperkeratosis. Using skin inflammation models such as IL-23 administration, imiquimod treatment, or mechanical irritation did not lead to exacerbated inflammation in the K14-flmIL-33 strain. As radiation induces a strong dermatitis due to apoptosis and necrosis, we determined the effect of fractionated radiation (12 Gy, 4 times). In comparison to wild-type mice, an increase in ear thickness in flmIL-33 transgenic mice was observed 25 days after irradiation. Macroscopic examination showed more severe skin symptoms in irradiated ears compared to controls. In summary, secreted mmIL-33 itself has a potent capacity in skin inflammation whereas fl IL-33 is limited due to its intracellular retention. During tissue damage, fl IL-33 exacerbated radiation-induced skin reaction

Charge-Dependence of the Nucleon-Nucleon Interaction

Based upon the Bonn meson-exchange-model for the nucleon-nucleon ($NN$) interaction, we calculate the charge-independence breaking (CIB) of the $NN$ interaction due to pion-mass splitting. Besides the one-pion-exchange (OPE), we take into account the $2\pi$-exchange model and contributions from three and four irreducible pion exchanges. We calculate the CIB differences in the $^1S_0$ effective range parameters as well as phase shift differences for partial waves up to total angular momentum J=4 and laboratory energies below 300 MeV. We find that the CIB effect from OPE dominates in all partial waves. However, the CIB effects from the $2\pi$ model are noticable up to D-waves and amount to about 40% of the OPE CIB-contribution in some partial waves, at 300 MeV. The effects from 3$\pi$ and 4$\pi$ contributions are negligible except in $^1S_0$ and $^3P_2$.Comment: 12 pages, RevTex, 14 figure