On the Initial Mass Function of Population III Stars

The collapse and fragmentation of filamentary primordial gas clouds are explored using 1D and 2D hydrodynamical simulations coupled with the nonequilibrium processes of H2 formation. The simulations show that depending upon the initial density,there are two occasions for the fragmentation of primordial filaments. If a filament has relatively low initial density, the radial contraction is slow due to less effective H2 cooling. This filament tends to fragment into dense clumps before the central density reaches $10^{8-9}$ cm$^{-3}$, where H2 cooling by three-body reactions is effective and the fragment mass is more massive than some tens $M_\odot$. In contrast, if a filament is initially dense, the more effective H2 cooling with the help of three-body reactions allows the filament to contract up to $n\sim 10^{12}$ cm$^{-3}$. After the density reaches $n\sim 10^{12}$ cm$^{-3}$, the filament becomes optically thick to H2 lines and the radial contraction subsequently almost stops. At this final hydrostatic stage, the fragment mass is lowered down to $\approx 1M_\odot$ because of the high density of the filament. The dependence of the fragment mass upon the initial density could be translated into the dependence on the local amplitude of random Gaussian density fields or the epoch of the collapse of a parent cloud. Hence, it is predicted that the initial mass function of Population III stars is likely to be bimodal with peaks of $\approx 10^2 M_\odot$ and $\approx 1M_\odot$, where the relative heights could be a function of the collapse epoch.Comment: Accepted by Ap

Fire Suppression Impacts on Fuels and Fire Intensity in the Western U.S.: Insights from Archaeological Luminescence Dating in Northern New Mexico

Here, we show that the last century of fire suppression in the western U.S. has resulted in fire intensities that are unique over more than 900 years of record in ponderosa pine forests (Pinus ponderosa). Specifically, we use the heat-sensitive luminescence signal of archaeological ceramics and tree-ring fire histories to show that a recent fire during mild weather conditions was more intense than anything experienced in centuries of frequent wildfires. We support this with a particularly robust set of optically stimulated luminescence measurements on pottery from an archaeological site in northern New Mexico. The heating effects of an October 2012 CE prescribed fire reset the luminescence signal in all 12 surface samples of archaeological ceramics, whereas none of the 10 samples exposed to at least 14 previous fires (1696–1893 CE) revealed any evidence of past thermal impact. This was true regardless of the fire behavior contexts of the 2012 CE samples (crown, surface, and smoldering fires). It suggests that the fuel characteristics from fire suppression at this site have no analog during the 550 years since the depopulation of this site or the 350 years of preceding occupation of the forested landscape of this region

Lysophosphatidic Acid Acyltransferase β (LPAATβ) Promotes the Tumor Growth of Human Osteosarcoma

Osteosarcoma is the most common primary malignancy of bone with poorly characterized molecular pathways important in its pathogenesis. Increasing evidence indicates that elevated lipid biosynthesis is a characteristic feature of cancer. We sought to investigate the role of lysophosphatidic acid acyltransferase β (LPAATβ, aka, AGPAT2) in regulating the proliferation and growth of human osteosarcoma cells. LPAATβ can generate phosphatidic acid, which plays a key role in lipid biosynthesis as well as in cell proliferation and survival. Although elevated expression of LPAATβ has been reported in several types of human tumors, the role of LPAATβ in osteosarcoma progression has yet to be elucidated.Endogenous expression of LPAATβ in osteosarcoma cell lines is analyzed by using semi-quantitative PCR and immunohistochemical staining. Adenovirus-mediated overexpression of LPAATβ and silencing LPAATβ expression is employed to determine the effect of LPAATβ on osteosarcoma cell proliferation and migration in vitro and osteosarcoma tumor growth in vivo. We have found that expression of LPAATβ is readily detected in 8 of the 10 analyzed human osteosarcoma lines. Exogenous expression of LPAATβ promotes osteosarcoma cell proliferation and migration, while silencing LPAATβ expression inhibits these cellular characteristics. We further demonstrate that exogenous expression of LPAATβ effectively promotes tumor growth, while knockdown of LPAATβ expression inhibits tumor growth in an orthotopic xenograft model of human osteosarcoma.Our results strongly suggest that LPAATβ expression may be associated with the aggressive phenotypes of human osteosarcoma and that LPAATβ may play an important role in regulating osteosarcoma cell proliferation and tumor growth. Thus, targeting LPAATβ may be exploited as a novel therapeutic strategy for the clinical management of osteosarcoma. This is especially attractive given the availability of selective pharmacological inhibitors

Spatial Variability of Levees as Measured Using the CPT

The spatial variability of a soil deposit is something that is commonly discussed but difficult to quantify. The heterogeneity as a function of lateral distance can be critical to the design of long engineered structures such as highways, bridges, levees, and other lifelines. This paper presents a methodology for using CPT measurements to quantifying the spatial variability of cone tip resistance along a levee in the California Bay Delta. The results, presented in the form of a general relative variogram, identify the distance at which the maximum spatial variability is achieved for a given soil strata. This information helps define minimally correlated stretches of levee for proper failure and risk analysis. Presented herein are methods of interpreting, calculating, and analyzing CPT data to arrive at the quantified spatial variability with respect to different static and seismic failure modes common to levee systems

Localization and Characterization of the 86- and 84-kDa Heat Shock Proteins in Hepa 1c1c7 Cells

The 90-kDa heat shock protein (hsp90) is present in cells at high levels in the cytoplasm and is composed of two separate gene products, hsp86 and hsp84. Rabbit polyclonal antibodies to the murine N-terminal sequences of the 86- and 84-kDa heat shock proteins were isolated from serum by peptide affinity chromatography, Antibodies against each form of hsp90 are capable of immunoprecipitating hsp90. Each antibody preparation is specific against either hsp86 or hsp84 when tested on a protein blot of Hepa 1c1c7 cytosol. The overall ratio of hsp84/hsp86 in Hepa 1 cytosol was estimated to be 2 to 1. Each antibody preparation was used to immunoprecipitate hsp84 or hsp86 from Hepa 1 cytosol to test whether hsp86/84 exists as a homo- and/or heterodimer. After electrophoresis, silver staining revealed that anti-hsp86 antibodies immunoprecipitated both hsp86 and hsp84. This result would suggest that hsp86 forms heterodimers with hsp84. In contrast, the anti-hsp84 antibodies immunoprecipitated almost entirely hsp84, suggesting that hsp84 exists largely as homodimers. Both anti-hsp86 and hsp84 antibodies were able to immunoprecipitate the 2-azido-3-[125I]iodo-7, 8-dibromodibenzo-p-dioxin-labeled Ah receptor from Hepa 1 cytosol, indicating that these antibodies are able to bind to hsp90 when it is complexed with other proteins. Both antibody preparations recognize hsp90 in mouse, rat, and human cell lines. Immunofluorescence and confocal microscopy were performed using both antibody preparations, and the results indicated that both hsp86 and hsp84 were located in the cytoplasm and nucleus of Hepa 1 cells. Hsp86 was found to localize unevenly in the cytoplasm, while hsp84 was found evenly dispersed throughout the cytoplasm. Hsp86 also appeared to be localized to a greater degree than hsp84 in the vicinity of the nuclear envelope.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/30416/1/0000036.pd