HPPD GENE EXPRESSION IN RELATION TO VITAMIN E CONTENT IN SPRING BARLEY

Abstract KOSAŘ, M., HOLKOVÁ, L., BŘEZINOVÁ BELCREDI, N., EHRENBERGEROVÁ, J.: HPPD gene expression in relation to vitamin E content in spring barley. Acta univ. agric. et silvic. Mendel. Brun., 2009, LVII, No. 4, pp. 13-18 The enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD) has a very important role in the biosynthetic pathway of vitamin E. Its activity infl uences the fi nal level of tocols in plant tissues. Seven barley cultivars with diff erent vitamin E level were grown under control conditions and activity of HPPD gene was measured four, eight and twelve days a er pollination of ear tissues. It was found that activity of HPPD gene corresponded with vitamin E content detected in grains (r = 0.77*). The relationship between the gene activity for HPPD eight and twelve days a er pollination and vitamin E content was also confi rmed for analyzed cultivars grown in the fi eld conditions (r = 0.85*)

The effect of the genotype and environment on damage of barley grains (Hordeum vulgare L.)

The occurrence of damaged grains was studied in 12 barley genotypes in a three-year period. The occurrence of grains with physiological split of lemma and endosperm was generally low. Split of palea and endosperm was mostly affected by the genotype (10%) and it was recorded most frequently in the genotype Heris. Incomplete overlapping of husks was also markedly affected by the genotype (10%) and it occurred most frequently in the genotype Scarlett. Sprouted grains and grains of green color occurred minimally. Black points were affected by year (21%) and genotype (15%). This damage was most frequently reported for the genotypes Camera and Jersey. Surprisingly, the occurrence of grains with apparently moldy husks was markedly affected by the genotype (25%), less substantially by the location (10%) and minimally by year (3%)

Healthy substances in barley and beer.

The steadily improved care of health leads to an increased interest in healthy nourishment. Apart from other angles, the nutriments and their raw materials are evaluated in compliance with the content of nutritively valuable substances. Barley, malt, malt extract and beer are sources of a number of chemical compounds that without any doubt have positive action on human organism - mainly the substances with antioxidant effect and some forms of dietetic fibres. In its department, the Malting Institute in Brno, the VUPS Ltd. engages in determination of content of tocols and b-glucans in individual grains of barley in dependence on the variety and genetic characters of the materials.The steadily improved care of health leads to an increased interest in healthy nourishment. Apart from other angles, the nutriments and their raw materials are evaluated in compliance with the content of nutritively valuable substances. Barley, malt, malt extract and beer are sources of a number of chemical compounds that without any doubt have positive action on human organism - mainly the substances with antioxidant effect and some forms of dietetic fibres. In its department, the Malting Institute in Brno, the VUPS Ltd. engages in determination of content of tocols and b-glucans in individual grains of barley in dependence on the variety and genetic characters of the materials