Convergent evolution of bacterial ceramide synthesis

The bacterial domain produces numerous types of sphingolipids with various physiological functions. In the human microbiome, commensal and pathogenic bacteria use these lipids to modulate the host inflammatory system. Despite their growing importance, their biosynthetic pathway remains undefined since several key eukaryotic ceramide synthesis enzymes have no bacterial homologue. Here we used genomic and biochemical approaches to identify six proteins comprising the complete pathway for bacterial ceramide synthesis. Bioinformatic analyses revealed the widespread potential for bacterial ceramide synthesis leading to our discovery of the first known Gram-positive species to produce ceramides. Biochemical evidence demonstrated that the bacterial pathway operates in a different order than in eukaryotes. Furthermore, phylogenetic analyses support the hypothesis that the bacterial and eukaryotic ceramide pathways evolved independently

DIC and Photogrammetry for Structural Dynamic Analysis and High-Speed Testing

This chapter provides an overview and some important considerations to be made when making optical and stereophotogrammetry measurements on structures for dynamic applications. In particular, the chapter focuses on leveraging those measurements to perform digital image correlation (DIC) to extract dynamic parameters (e.g., strain, deflection, operating shapes, and mode shapes). Structural dynamic testing and analysis in the context of performing optical measurements is described. Information on optical high rate testing is also presented along with lessons learned and best practices

Innovation in hospitals: a survey of the literature

Innovation, Hospitals, Survey, O3, I1,