Effect of Origanum heracleoticum L. essential oil on food-borne Penicillium aurantiogriseum and Penicilium chrysogenum isolates

Molds are ubiquitously distributed in nature and their spores can be found in the atmosphere even at high altitudes. The difficulty of controlling these undesirable molds, as well as the growing interest of the consumers in natural products, have been forcing the industry to find new alternatives for food preservation. The modern trends in nutrition suggest the limitation of synthetic food additives or substitution with natural ones. Aromatic herbs are probably the most important source of natural antimicrobial agents. Origanum heracleoticum L. essential oil has been known as an interesting source of antimicrobial compounds to be applied in food preservation. In the this work, we have investigated the effect of essential oil obtained from O. heracleoticum on growth of six isolates of Penicillium aurantiogriseum and four isolates of Penicillium chrysogenum isolated from meat plant for traditional Petrovacka sausage (Petrovská klobása) production. The findings reveal that the essential oil of O. heracleoticum provides inhibition of all of fungal isolates tested. O. heracleoticum L. essential oil exhibited higher antifungal activity against the isolates of P. chrysogenum than the isolates of P. aurantiogriseum. O. heracleoticum essential oil showed a MIC value ranging from 25 to 100 μL/mL. The fungi cultivated in the medium with higher concentration of essential oil showed certain morphological changes. The alterations included lack of sporulation and loss of pigmentation

Povezanost sniženoga salivarnog melatonina i narušene kvalitete života u bolesnika s kroničnom spontanom urtikarijom - probno istraživanje

Chronic spontaneous urticaria (CSU) is a dermatological disorder accompanied by itching that greatly affects the quality of life and quality of sleep. Therefore, it is assumed that CSU patients consequently experience reduced melatonin secretion and lower values of serum or salivary melatonin. This pilot study included 20 patients with CSU (chronic urticaria of unknown etiology that lasts for more than 6 weeks) and 10 healthy controls. All subjects were examined by a dermatovenereologist-allergist, as well as an oral pathologist, to exclude oral pathological conditions. Salivary melatonin levels were determined by ELISA and all subjects completed a standardized Dermatology Life Quality Index questionnaire and Pittsburgh Sleep Quality Index on the same day they gave a saliva sample for analysis. According to our results, 86% of CSU patients had decreased values of salivary melatonin, and lower salivary melatonin values significantly correlated with a reduced quality of life in CSU patients. This study was the first to analyze melatonin in CSU patients, also suggesting a possible new therapeutic option for the treatment of CSU.Kronična spontana urtikarija (KSU) je bolest kože praćena intenzivnim subjektivnim osjećajem svrbeža koji snažno utječe na kvalitetu života i kvalitetu spavanja bolesnika. Pretpostavka je da bolesnici s KSU-om posljedično imaju smanjeno lučenje melatonina, kao i niže vrijednosti melatonina u serumu ili slini. Ovo probno istraživanje uključilo je 20 bolesnika s KSU-om (urtikarija koja traje dulje od 6 tjedana nepoznate etiologije) i 10 zdravih ispitanika. Svakog bolesnika je pregledao dermatovenerolog-alergolog, kao i oralni patolog koji je isključio bolesti usne šupljine koje bi mogle utjecati na razinu melatonina u bolesnika. Razina salivarnog melatonina određena je testom ELISA, a svi ispitanici su ispunili standardizirani dermatološki upitnik o kvaliteti života (Dermatology Life Quality Index, DLQI) i Pittsburški upitnik o kvaliteti sna (Pittsburgh Sleep Quality Index, PSQI) istoga dana kad im je uzet uzorak sline za analizu. Prema rezultatima ovog istraživanja 86% bolesnika s KSU-om imalo je snižene vrijednosti salivarnog melatonina koje značajno koreliraju s lošijom kvalitetom života u bolesnika s KSU-om. Ovo istraživanje je prvo istraživanje koje je analiziralo vrijednosti melatonina u bolesnika s KSU-om i koje predlaže novu terapijsku mogućnost u liječenju KSU-e

Gingival Necrosis Caused by an Ill-Fitting Denture

U ovom prikazu opisali smo slučaj 80-godišnjeg pacijenta koji je bio upućen u Zavod za oralnu medicinu Stomatološkog fakulteta u Zagrebu zbog gingivalnog ulkusa prisutnog osam dana. Na kliničkom pregledu uočena je eksponirana kost na bezubom alveolarnom grebenu u području molara s desne strane mandibule veličine 0,8 cm u promjeru. Inače, pacijent je svakodnevno uzimao doksazosin jer je imao teškoća s urinarnim traktom te ipatropij-bromid zbog respiratornih tegoba. Donju djelomičnu protezu nije promijenio već šest godina. Na početku je, točnije prva tri dana, bio liječen parodontnim zavojem (Resopack, HagenWerken, Njemačka) uz preporuku da ne nosi protezu, no nakon tri dana klinički pregled nije pokazao poboljšanje stanja. Zato smo se odlučili na liječenje topikalnim kortikosteroidom (betametazon) i oralnim antiseptikom (klorheksidin-diglukonat) tri puta na dan. Nakon tri tjedna lezija je zacijelila. Naveden je popis mogućih čimbenika koji mogu rezultirati nastankom ulkusa gingive.We present a case of an 80-year-old male who was referred to the Department of Oral Medicine, School of Dental Medicine University of Zagreb, Croatia due to gingival ulcer which was present for eight days. Clinical examination has revealed exposed bone on the toothless alveolar ridge in the lower molar region on the right side of 0.8 cm in diameter. Otherwise, the patient was taking doxazosin due to urinary problems and ipatropium bromide due to respiratory problems. The patient wore a 6-year-old partial lower denture. He was initially treated with periodontal bandage (Resopack, HagenWerken, Germany) for the first three days and was instructed not to wear the denture; however, no benefit could be seen. Therefore, we added a local corticosteroid (betamethasone) and an oral antiseptic (chlorhexidine digluconate) applied three times a day. After 3 weeks the lesion healed. A list of possible causative factors regarding gingival ulcers is included

Sindrom pekućih usta – goruća enigma

Burning Mouth Syndrome (BMS) is a chronic pain condition characterized by an intraoral burning sensation and an absence of oral mucosal lesions and disturbances in laboratory findings. Burning symptoms usually affect the anterior two-thirds of the tongue, its lateral borders, hard palate and labial mucosa, but other oral cavity sites may also be affected. Taste alterations and a decrease in the salivary flow rate frequently accompany the burning symptoms. This condition mostly affects peri- and postmenopausal women. To date, the etiology of BMS remains unclear. This unknown etiology means that no appropriate treatment is currently available. A large number of the treatments and medications have been tried for BMS, but treatment management remains unsatisfactory in some patients. The purpose of this article is to present current knowledge on the treatment of BMS.Sindrom pekućih usta (SPU) kronično je bolno stanje koje je karakterizirano osjećajem žarenja u usnoj šupljini bez vidljivih promjena na sluznici i poremećaja u laboratorijskim nalazima. Simptomi žarenja obično zahvaćaju prednje 2/3 trećine i lateralne površine jezika, tvrdo nepce, labijalnu sluznicu, ali može biti zahvaćena bilo koja regija u usnoj šupljini. Simptomi žarenja obično su praćeni promjenom osjeta okusa te smanjenim lučenjem sline. Ovo stanje najviše pogađa žene u peri- i postmenopauzi. S obzirom na to da je etiologija SPU-a nepoznata, ne postoji niti odgovarajuće liječenje. Dostupno je više različitih terapija i lijekova za SPU, međutim, liječenje kod pojedinih pacijenata i dalje je bezuspješno. Svrha ovog rada je prikazati trenutno znanje o liječenju SPU-a

Suvremeni pristup liječenju oralnog mukozitisa: pregledni rad

Oral mucositis (OM) is a serious inflammation of the mucosa and occurs in patients with head and neck cancer (HNC) who are being treated with radiotherapy (RT) and/or chemotherapy (CT), and in patients undergoing hematopoietic stem cell transplantation (HSCT). The inflammation accompanied with painful ulcerations inside the oral cavity impairs its function making the basic functions like eating and speech hard or even impossible. It usually occurs at the end of the first week of RT and lasts for several weeks after cessation. The intensity of OM can sometimes lead to discontinuation of RT. The degree of mucositis regarding chemotherapy depends on the type of antineoplastic drug, therapeutic procedure, duration of the therapy and dose, as well as previous exposure of the oral cavity to toxic agents. Prolonged or repeated administration of lower doses of chemotherapy is associated with a higher chance of developing oral mucositis comparing to a bolus, while chronomodulation of chemotherapy reduces the possibility of mucositis without affecting antineoplastic activity. The treatment of OM is symptomatic, as there is still no effective treatment. In this review paper, several contemporary options for alleviating the symptoms of oral mucositis are listed.Oralni mukozitis (OM) ozbiljna je upala sluznice i javlja se u bolesnika s karcinomom glave i vrata (HNC) koji se liječe radioterapijom (RT) i/ili kemoterapijom (CT) te u bolesnika koji su podvrgnuti transplantaciji hematopoetskih matičnih stanica (HSCT). Upala praćena bolnim ulceracijama unutar usne šupljine narušava njezinu funkciju, čineći osnovne funkcije poput prehrane i govora teškim ili čak nemogućim. Obično se javlja na kraju prvog tjedna radioterapije i traje nekoliko tjedana nakon prestanka. Intenzitet oralnog mukozitisa ponekad može dovesti do prekida radioterapije. Stupanj mukozitisa kod kemoterapije ovisi o vrsti antineoplastičnog lijeka, terapijskom postupku, trajanju terapije i dozi, kao i o prethodnoj izloženosti usne šupljine toksičnim agensima. Produljena ili ponovljena primjena nižih doza kemoterapije povezana je s većom šansom za razvoj oralnog mukozitisa u usporedbi s bolusom, dok kronomodulacija kemoterapije smanjuje mogućnost mukozitisa bez utjecaja na antineoplastično djelovanje. Liječenje oralnog mukozitisa je simptomatsko jer još uvijek nema učinkovitog lijeka. U ovom preglednom radu navodi se nekoliko suvremenih opcija za ublažavanje simptoma oralnog mukozitisa

Oral and vulvar lichen sclerosus

No abstract availabl

Oral and vulvar lichen sclerosus

No abstract availabl

Lijekovima izazvane oralne ulceracije: prikaz slučaja

A 70-year-old patient was admitted to the Department of Oral Medicine for multiple oral ulcerations on the left buccal mucosa, around 0.5 cm in diameter, as well as on the gingiva. Otherwise, the patient suffered from chronic lymphocytic leukemia, hypogammaglobulinemia, chronic renal insufficiency, with complete afunction of the right kidney, asthma, hypertension, gastritis and prostate hyperplasia. Differential diagnosis of oral ulcerations included drug induced oral ulcerations, paraneoplastic pemphigus, viral ulcerations (cytomegalovirus, herpes simplex viruses), fungal ulcerations (candidiasis, aspergillosis, histoplasmosis, cryptococcosis) and bacterial ulcerations, as well as neutropenic ulcers. One of the possible explanations was that the lesions were due to the use of drugs, the more so as oral lesions evolved when the doses of allopurinol and chlorambucil were increased, and subsided when the doses of both drugs were decreased. However, we could not establish for sure whether the lesions were due to allopurinol or chlorambucil. According to literature data, allopurinol is one of the most frequent drugs known to induce skin adverse reactions, therefore we assumed that it was the culprit drug. Unfortunately, several weeks later the patient died from sepsis, pneumonia with respiratory insufficiency and multiorgan failure.Bolesnik u dobi od 70 godina upućen je na Zavod za oralnu medicinu zbog nekoliko oralnih ulceracija na lijevoj obraznoj sluznici veličine oko pola centimetra u promjeru te na gingivi. Inače je bolovao od kronične limfocitne leukemije, hipogamaglobulinemije, kronične bubrežne insufi cijencije s potpunom afunkcijom desnog bubrega, astme, hipertenzije, gastritisa i hiperplazije prostate. Diferencijalna dijagnoza oralnih ulceracija uključivala je oralne ulceracije uslijed uzimanja lijekova, paraneoplastični pemfi gus, virusne ulceracije (citomegalovirus, herpes simpleks viruse), gljivične ulceracije (kandidijaza, aspergiloza, histoplazmoza, kriptokokoza), bakterijske ulceracije te neutropenične ulceracije. Jedno od mogućih objašnjenja je bilo da su lezije vjerojatno nastale uslijed uzimanja lijekova, jer su se pojavile kada se doza alopurinola i klorambucila povećala, a počele su nestajati kada se doza tih lijekova smanjila. Ipak, nismo sigurni koji je od ova dva lijeka doveo do nastanka lezija, jer je nažalost nekoliko tjedana poslije bolesnik izdahnuo uslijed sepse, pneumonije i respiracijske insuficijencije te višeorganskog zatajenja

Simultana pojava oralnog i kožnog anaplastičnog limfoma velikih T stanica

We present case of oral and skin anaplastic T-cell lymphoma in a 68-year-old woman. The patient presented with extensive ulcerations and necrotic tissue on the left mandibular gingiva. Orthopantomogram finding showed extensive necrolytic lesions of the adjacent mandible. Biopsy finding of oral lesions and subsequently of the skin confirmed the diagnosis of anaplastic T-cell lymphoma. The bridge on the teeth 35-37 was taken out. After three cycles of chemotherapy, oral lesions subsided, unlike skin lesions. Dentists should be aware that differential diagnosis when dealing with oral ulcerations might be the result of certain malignant hematologic diseases.U radu se prikazuje slučaj oralnog i kožnog anaplastičnog limfoma velikih T stanica u 68-godišnje osobe. U bolesnice su na prvom pregledu uočene opsežne ulceracije i nekrotično tkivo u području gingive na mandibuli lijeve strane lica. Na ortopantomogramu su se vidjele opsežne nekrolitične lezije na kosti mandibule toga dijela. Patohistološki nalaz sluznice usne šupljine, a poslije i kože potvrdio je dijagnozu anaplastičnog limfoma velikih T stanica. Most na zubima 35-37 je izvađen. Nakon tri ciklusa kemoterapije oralne lezije su se povukle, za razliku od kožnih lezija. Stomatolozi pri diferencijalnoj dijagnostici oralnih ulceracija moraju imati na umu i moguću povezanost tih lezija s malignim hematološkim bolestima

Simultana pojava oralnog i kožnog anaplastičnog limfoma velikih T stanica

We present case of oral and skin anaplastic T-cell lymphoma in a 68-year-old woman. The patient presented with extensive ulcerations and necrotic tissue on the left mandibular gingiva. Orthopantomogram finding showed extensive necrolytic lesions of the adjacent mandible. Biopsy finding of oral lesions and subsequently of the skin confirmed the diagnosis of anaplastic T-cell lymphoma. The bridge on the teeth 35-37 was taken out. After three cycles of chemotherapy, oral lesions subsided, unlike skin lesions. Dentists should be aware that differential diagnosis when dealing with oral ulcerations might be the result of certain malignant hematologic diseases.U radu se prikazuje slučaj oralnog i kožnog anaplastičnog limfoma velikih T stanica u 68-godišnje osobe. U bolesnice su na prvom pregledu uočene opsežne ulceracije i nekrotično tkivo u području gingive na mandibuli lijeve strane lica. Na ortopantomogramu su se vidjele opsežne nekrolitične lezije na kosti mandibule toga dijela. Patohistološki nalaz sluznice usne šupljine, a poslije i kože potvrdio je dijagnozu anaplastičnog limfoma velikih T stanica. Most na zubima 35-37 je izvađen. Nakon tri ciklusa kemoterapije oralne lezije su se povukle, za razliku od kožnih lezija. Stomatolozi pri diferencijalnoj dijagnostici oralnih ulceracija moraju imati na umu i moguću povezanost tih lezija s malignim hematološkim bolestima