Low Cycle Fatigue Damage of Mod.9Cr-1Mo Steel under Non-Proportional Multiaxial Loading

AbstractThis study discusses multiaxial low cycle fatigue damage of Mod.9Cr-1Mo steel under proportional and non-proportional loadings at room and high (823K) temperatures. Strain-controlled multiaxial low cycle fatigue tests were carried out using a hollow cylinder specimen including interruption tests. Strain paths employed were a push-pull straining, a reversed torsion straining and a circle straining. Behaviors of cyclic deformations and failure lives and an evaluation of the failure life are discussed. Surface cracks and microstructures in fatigued specimens are also observed by a digital microscope and a transmission electron microscope (TEM) in order to investigate mechanisms of cyclic deformation, failure and reduction in failure life due to non-proportional loading

Quick-drying, protective coating of papers with poly(methacrylate) latices containing alkali-hydrolysable emulsifiers

Surfaces of filter papers pretreated with sodium carbonate were coated with poly(methacrylate) latices containing alkali-hydrolysable or non-hydrolysable cationic emulsifiers by a simple drop-coating method and their surface properties were investigated. Determination of fixed amounts of polymers and observation of the paper surfaces suggest that glass transition temperature of the polymers and hydrolysability of the emulsifier are dominant factors for the coating. The latices of poly(2-ethylhexyl methacrylate) containing the hydrolysable emulsifier could coat the paper surface almost perfectly and smoothly, making it quick-drying and water repellent.ArticlePOLYMER BULLETIN. 67(7):1367-1377 (2011)journal articl

Evaluation of wood preservatives against the drywood termite, Incisitermes minor

Tese de doutoramento em Arte Contemporânea, apresentada ao Colégio das Artes da Universidade de Coimbra

Development of a 1,3a,6a-triazapentalene derivative as a compact and thiol-specific fluorescent labeling reagent

For the fluorescence imaging of biologically active small compounds, the development of compact fluorophores that do not perturb bioactivity is required. Here we report a compact derivative of fluorescent 1,3a,6a-triazapentalenes, 2-isobutenylcarbonyl-1,3a,6a-triazapentalene (TAP-VK1), as a fluorescent labeling reagent. The reaction of TAP-VK1 with various aliphatic thiols proceeds smoothly to afford the corresponding 1,4-adducts in high yields, and nucleophiles other than thiols do not react. After the addition of thiol groups in dichloromethane, the emission maximum of TAP-VK1 shifts to a shorter wavelength and the fluorescence intensity is substantially increased. The utility of TAP-VK1 as a compact fluorescent labeling reagent is clearly demonstrated by the labeling of Captopril, which is a small molecular drug for hypertension. The successful imaging of Captopril, one of the most compact drugs, in this study demonstrates the usefulness of compact fluorophores for mechanistic studies

Traceless synthesis of protein thioesters using enzyme-mediated hydrazinolysis and subsequent self-editing of cysteinyl prolyl sequence

A traceless thioester-producing protocol featuring carboxypeptidase Y-mediated hydrazinolysis of cysteinyl prolyl leucine-tagged peptides has been developed. The hydrazinolysis followed by thioesterification affords cysteinyl prolyl thioesters. Self-editing of the tag and subsequent trans-thioesterification yields peptide thioesters. The developed protocol was successfully applied to conversion of recombinant proteins to thioesters