Infrared Search for Young Brown Dwarf Companions around Young Stellar Objects in the rho Ophiucus Molecular Cloud and the Serpens Molecular Cloud

We conducted an infrared search for faint companions around 351 young stellar objects in the rho Ophiucus molecular cloud and the Serpens molecular cloud. Nine objects in the Spitzer/IRAC archival images were identified as young stellar companion candidates. They showed an intrinsic infrared excess; one object was extremely red both in the [3.6] - [4.5] color and in the [4.5] - [5.8] color, and two objects were red in the [4.5] - [5.8] color. They were as faint as 15 mag in the [3.6] band. Follow-up K-band spectroscopy revealed that three objects had deep water absorption bands, indicative of low effective temperatures. By comparing the spectra and infrared spectral energy distributions with synthesized spectra of low-temperature objects, we derived the effective temperatures and continuum excess for these objects. It seems highly likely that one of the three objects is a low-mass stellar companion and two objects are young brown dwarf companions associated with the young stellar objects.Comment: 11 pages, 7 figures. P.A.S.J. accepte

Traceless synthesis of protein thioesters using enzyme-mediated hydrazinolysis and subsequent self-editing of cysteinyl prolyl sequence

A traceless thioester-producing protocol featuring carboxypeptidase Y-mediated hydrazinolysis of cysteinyl prolyl leucine-tagged peptides has been developed. The hydrazinolysis followed by thioesterification affords cysteinyl prolyl thioesters. Self-editing of the tag and subsequent trans-thioesterification yields peptide thioesters. The developed protocol was successfully applied to conversion of recombinant proteins to thioesters

Report on the Launch of a New Course <Integrated English>

This project aims to establish the environmental goals of a new course called and to find suitable materials for it. We also investigate similar courses in other major Japanese universities. The aims are: 1. To collect and study reference books concerning innovation in English education in Japan; 2. To establish the concept of clearly; 3. To work out the content and teaching methods for ; 4. To form some syllabi for several prototype classes; 5. To identify possible problems in practice; 6. To find ways to evaluate the class; The example syllabi in this report are as follows: 1. English in environmental studies; 2. English as a language for global communication; 3. English in movies; 4. Introduction to men's studies; In order to clarify the idea of , we include as an appendix a brief document on the history of innovation of English language education at Hiroshima University

Post-Exercise Muscle Protein Synthesis in Rats after Ingestion of Acidified Bovine Milk Compared with Skim Milk

Bovine milk proteins have a low absorption rate due to gastric acid-induced coagulation. Acidified milk remains liquid under acidic conditions; therefore, the absorption rate of its protein may differ from that of untreated milk. To investigate how this would affect muscle protein synthesis (MPS), we compared MPS after ingestion of acidified versus skim milk in rats. Male Sprague-Dawley rats swam for 2 h and were immediately administered acidified or skim milk, then euthanized at 30, 60, 90, and 120 min afterwards. Triceps muscle samples were excised for assessing fractional synthetic rate (FSR), plasma components, intramuscular free amino acids and mTOR signaling. The FSR in the acidified milk group was significantly higher than in the skim milk group throughout the post-ingestive period. Plasma essential amino acids, leucine, and insulin levels were significantly increased in the acidified milk group at 30 min after administration compared to the skim milk group. In addition, acidified milk ingestion was associated with greater phosphorylation of protein kinase B (Akt) and ribosomal protein S6 kinase (S6K1), and sustained phosphorylation of 4E-binding protein 1 (4E-BP1). These results indicate that compared with untreated milk, acidified milk ingestion is associated with greater stimulation of post-exercise MPS

Effects of Whey, Caseinate, or Milk Protein Ingestion on Muscle Protein Synthesis after Exercise

Whey protein (WP) is characterized as a “fast” protein and caseinate (CA) as a “slow” protein according to their digestion and absorption rates. We hypothesized that co-ingestion of milk proteins (WP and CA) may be effective for prolonging the muscle protein synthesis response compared to either protein alone. We therefore compared the effect of ingesting milk protein (MP) to either WP or CA alone on muscle protein synthesis after exercise in rats. We also compared the effects of these milk-derived proteins to a control, soy protein (SP). Male Sprague-Dawley rats swam for two hours. Immediately after exercise, one of the following four solutions was administered: WP, CA, MP, or SP. Individual rats were euthanized at designated postprandial time points and triceps muscle samples collected for measurement of the protein fractional synthesis rate (FSR). FSR tended to increase in all groups post-ingestion, although the initial peaks of FSR occurred at different times (WP, peak time = 60 min, FSR = 7.76%/day; MP, peak time = 90 min, FSR = 8.34%/day; CA, peak time = 120 min, FSR = 7.85%/day). Milk-derived proteins caused significantly greater increases (p &lt; 0.05) in FSR compared with SP at different times (WP, 60 min; MP, 90 and 120 min; CA, 120 min). Although statistical analysis could not be performed, the calculated the area under the curve (AUC) values for FSR following this trend were: MP, 534.61; CA, 498.22; WP, 473.46; and SP, 406.18. We conclude that ingestion of MP, CA or WP causes the initial peak time in muscle protein synthesis to occur at different times (WP, fast; MP, intermediate; CA, slow) and the dairy proteins have a superior effect on muscle protein synthesis after exercise compared with SP