Constitutive CD8 expression allows inefficient maturation of CD4+ helper T cells in class II major histocompatibility complex mutant mice.

Although mature CD4+ T cells bear T cell receptors (TCRs) that recognize class II major histocompatibility complex (MHC) and mature CD8+ T cells bear TCRs that recognize class I MHC, it is possible that the initial commitment of an immature thymocyte to a CD4 or CD8 lineage is made without regard to the specificity of the TCR. According to this model, CD4+ cells with class I TCR do not mature because the CD8 coreceptor is required for class I MHC recognition and positive selection. If this model is correct, constitutive expression of CD8 should allow CD4+ T cells with class I-specific TCRs to develop. In this report, we show that mature peripheral CD4+ cells are present in class II MHC-deficient mice that express a constitutive CD8.1 transgene. These cells share a number of properties with the major class II MHC-selected CD4 population, including the ability to express CD40 ligand upon activation. Although mature CD4 cells are also detectable in the thymus of class II MHC mutant/CD8.1 transgenic mice, they represent a small fraction of the mature CD4 cells found in mice that express class II MHC. These results indicate that some T cells choose the CD4 helper lineage independent of their antigen receptor specificity; however, the inefficiency of generating class I-specific CD4 cells leaves open the possibility that an instructive signal generated upon MHC recognition may bias lineage commitment

The cytoplasmic domain of CD4 promotes the development of CD4 lineage T cells.

Thymocytes must bind major histocompatibility complex (MHC) proteins on thymic epithelial cells in order to mature into either CD8+ cytotoxic T cells or CD4+ helper T cells. Thymic precursors express both CD8 and CD4, and it has been suggested that the intracellular signals generated by CD8 or CD4 binding to class I or II MHC, respectively, might influence the fate of uncommitted cells. Here we test the notion that intracellular signaling by CD4 directs the development of thymocytes to a CD4 lineage. A hybrid protein consisting of the CD8 extracellular and transmembrane domains and the cytoplasmic domain of CD4 (CD884) should bind class I MHC but deliver a CD4 intracellular signal. We find that expression of a hybrid CD884 protein in thymocytes of transgenic mice leads to the development of large numbers of class I MHC-specific, CD4 lineage T cells. We discuss these results in terms of current models for CD4 and CD8 lineage commitment

Strongly Coupled Quantum and Classical Systems and Zeno's Effect

A model interaction between a two-state quantum system and a classical switching device is analysed and shown to lead to the quantum Zeno effect for large values of the coupling constant k . A minimal piecewise deterministic random process compatible with the Liouville equation is described, and it is shown that 1/k can be interpreted as the jump frequency of the classical deviceComment: 9 page

NMR analogues of the quantum Zeno effect

We describe Nuclear Magnetic Resonance (NMR) demonstrations of the quantum Zeno effect, and discuss briefly how these are related to similar phenomena in more conventional NMR experiments.Comment: 8 pages including 4 figures; intended as a possible answer to Malcolm Levitt's question at the 2005 Magnetic Resonanace GRC: "What is the NMR analogue of the quantum Zeno effect?". In press at Physics Letters

Quantum Zeno Effect in Trapped Ions

A "continuous measurement" Quantum Zeno Effect (QZE) in the context of trapped ions is predicted. We describe the physical system and study its exact time evolution showing the appearance of Zeno Phenomena. New indicators for the occurrence of QZE in oscillatory systems are proposed and carefully discussed.Comment: 12 pages, 4 figure