Alien Registration- Arsenault, Isreal (Brunswick, Cumberland County)

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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus

Staphylococcus aureus is a major cause of hospital-acquired infections. The multi-drug resistant nature of certain S. aureus strains makes the discovery of new drug targets for S. aureus vital. A newly discovered virulence factor from S. aureus was described as an ortholog of NagD from E. coli, a member of the nitrophenyl phosphatase family of the HAD (Haloacid Dehalogenase) superfamily. This thesis will show that this virulence factor is not an ortholog of NagD UMPase from E. coli, but rather a phosphoglycolate phosphatase (PGPase). If phosphoglycolate accumulates in the cell, it will inhibit the glycolytic enzyme triose phosphate isomerase (TPI). In S. aureus, TPI also serves as an adhesion protein that can bind to host cells; phosphoglycolate would interfere with this adhesion process and thus make it harder for S. aureus to infect host cells. Thus, this S. aureus PGPase may act as a virulence factor by degrading the TPI inhibitor phosphoglycolate. We cloned the gene, expressed and purified the protein, and determined and characterized its activity. We have subcloned this PGPase into a His•Tag vector, purified the protein using nickel affinity and size exclusion chromatography, and characterized enzymatic activity, optimal conditions (substrate, pH, and metal usage), and kinetics

Threats of Terror: Objectives, Options and Obstructions in Moral Education

This article analyses the intellectual, religious, national and moral processes through which a democratic society has had to confront in its day-to-day routines under the ever-present threat of terror. It discusses the effects of the terror over the character of Israeli society and the critical debates in its system of education. As far as it can be ascertained through the observations in this study, the general publics’ attitude could be defined as a mildly moral realistic one: people think that terror and violence have objective foundations but certainly embody some subjective human conventions and beliefs

Bridging the Gap to Increasing the Number of Black Student into Graduate School: An Academic Advising Tool

One way to increase the “dismally low” number of Black students in graduate school is to take an appreciative approach for advisors to utilize. Given that academic advisors interact with black undergraduate students on academic matters, the purpose of this article is to provide those academic advisors with specific tools from the Appreciative Advising framework for encouraging Black students to pursue post-graduate degree opportunities