13 research outputs found
Nature of the Liability of Shareholders of a Corporation, Under Statute Imposing a Liability Additional to That for Stock Subscribed
Get PDFNature of the Liability of Shareholders of a Corporation, Under Statute Imposing a Liability Additional to That for Stock Subscribed
Get PDFScalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors
No full textSummary: Here, we present a protocol for producing 3D pancreatic-like organoids from human pluripotent stem cells in suspension bioreactors. We describe scalable techniques for generating 10,000–100,000 organoids that further mature in 4–5 weeks into α- and β-like cells with glucose-responsive insulin and glucagon release. We detail procedures for culturing, passaging, and cryopreserving stem cells as suspended clusters and specify growth media and differentiation factors for differentiation. Finally, we discuss functional assays for research applications.For complete details on the use and execution of this protocol, please refer to Alvarez-Dominguez et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics
Observer performance for adaptive, image-based denoising and filtered back projection compared to scanner-based iterative reconstruction for lower dose CT enterography
No full text
The role of gut microbiota (commensal bacteria) and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer: contribution of germ-free and gnotobiotic animal models of human diseases
No full text
