マレーシア産植物のアレロパシー性評価とアレロパシー性Goniothalamus andersonii J. Sinclairの天然除草剤への応用

京都大学0048新制・課程博士博士(農学)甲第21831号農博第2344号新制||農||1068(附属図書館)学位論文||H31||N5203(農学部図書室)京都大学大学院農学研究科地域環境科学専攻(主査)教授 平井 伸博, 准教授 赤松 美紀, 教授 縄田 栄治学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDFA

Transcriptomic evaluation of plant growth inhibitory activity of goniothalamin from the Malaysian medicinal plant goniothalamus andersonii

Goniothalamin produced by the Malaysian medicinal plant, Goniothalamus andersonii J. Sinclair, strongly inhibits plant growth. However, its mode of action has not been characterized at the gene expression level. We conducted DNA microarray assay to analyze the changes in early gene responses of Arabidopsis thaliana seedlings. After a 6-h exposure to goniothalamin, we observed an upregulation of genes highly associated with heat response, and 22 heat shock protein (AtHSP) genes were upregulated more than 50 fold. Together with these genes, we observed upregulation of the genes related to oxidative stress and protein folding. Also, the genes related to cell wall modification and cell growth, expansin (AtEXPA) genes, were significantly downregulated. The results suggested that goniothalamin induces oxidative stresses and inhibits the expression of cell wall-associated proteins resulting in growth inhibition of Arabidopsis seedlings