Analysis of Adipokines and some Steroid Hormones in Myocardial Infarction

الموت الشامل بسبب أمراض القلب والأوعية الدموية هو السبب السائد للوفاة، ومرض القلب الإقفاري هو السبب البارز. هناك ميل لزيادة حساسية الأساليب في المختبر المعاصر لتشخيص الأمراض القلبية الوعائية، وتقييم المفتاح كمؤشرات حيوية للأمراض القلبية الوعائية. المهمة العاجلة هي البحث عن مؤشرات مختلفة كأداة للكشف المبكر عن احتشاء عضلة القلب ومراقبته. ضمت هذه المحاولة ١١٧ متطوعًا، تم تسجيلهم من كلا الجنسين في الفئة العمرية من ٣٢ إلى ٦٤ عامًا. تم تصنيف المتطوعين إلى مجموعتين: ٦٧ مريضًا من احتشاء عضلة القلب، واحتضنت المجموعات الأخرى ٥٠ فردًا سليمًا. تم جمع عينات الدم وتوجيهها للتحليل الكيميائي الحيوي لتقييم مستويات هرمون الاستراديول والتستوستيرون والبروجسترون والأديبونكتين واللبتين والدهون في كل مجموعة. بناءً على التقييم الإحصائي لمعلمات المؤشر الحيوي، يمكن الإعلان عن الاستنتاج التالي من هذه الدراسة، وهو انخفاض كبير في مستويات هرمون التستوستيرون والبروتين  الدهني عالي الكثافة (P <0.001) في احتشاء عضلة القلب، ضمن P <0.061 المرتفعة بشكل ملحوظ من مستويات البروجسترون في عضلة القلب مرضى الاحتشاء بالمقارنة مع الأفراد الأصحاء. أشارت الاختبارات الكيميائية الحيوية المتبقية إلى مستويات مرتفعة بشكل ملحوظ في المرضى الذين يعانون من احتشاء عضلة القلب مثل استراديول (P <0.001) ، أديبونكتين (P <0.001)، ليبتين (P <0.001) ، الكلي الكوليسترولالكوليسترول (P <0.001) ، الدهون الثلاثية P) <0.001) ومستويات البروتين الدهني منخفض الكثافة ) P <0.001). تُظهر الأديبوكينات (أديبونكتين ، لبتين) وبعض هرمونات الستيرويد (استراديول ، هرمون التستوستيرون) أدوارًا حاسمة في تحسين أمراض التمثيل الغذائي والقلب والأوعية الدموية ويمكن استخدامها كمؤشر حيوي للتعرض لاحتشاء عضلة القلب، والسلوك الطبي والشدة. يقدم هذا الإقرار التشخيص المبكر للمرض وتطوره.The most common cause of death is cardiovascular disease (CVD), with ischemic heart disease being the most notable type.  There is a propensity to raise the sensitivity of methods in contemporary laboratory for diagnosing of CVD, and assessing key as CVD bio-indicators. The urgent task is to seek for different indicators as a hopeful tool for early detection and monitoring of myocardial infarction in blood samples only. This study comprised 117 Volunteers, recorded with both genders in the age range of 32-64 years old. The volunteers were categorized into two groups: 67patients of myocardial infarction, other group embraced 50 healthy individuals. The samples of blood were collected and directed for biochemical analysis to evaluate estradiol, testosterone, progesterone, adiponectin, leptin, and lipid profile [total cholesterol, triglycerides, high density lipoprotein (HDL), and low-density lipoprotein (LDL)] levels in each group. The following conclusion can be drawn from this study based on statistical assessment of bio-indicator parameters, significantly reduced of testosterone and HDL (P <0.001) levels in myocardial infarction, within non-significantly elevated P <0.061 of progesterone levels in myocardial infarction patients as compared with healthy individuals. The remaining biochemical tests indicated significantly elevated levels in patients with myocardial infarction such as estradiol (P <0.001), adiponectin (P <0.001), leptin (P <0.001), total cholesterol (P <0.001), triglycerides (P <0.001) and LDL (P <0.001) levels. Adipokines (adiponectin, leptin) and some steroid hormones (estradiol, testosterone) show crucial roles in the improvement of metabolic and cardiovascular diseases and may be utilized as bio-pointer for myocardial infarction exposure, medical conduct and severity. This acknowledgment offers early diagnosis of disease and progression.

Alteration of Serum Immunoglobulin Levels in Woman with Ovarian Cancer

Ovarian cancer has a high mortality and delayed diagnosis. Several immunological alterations take place during ovarian carcinogenesis, and can be of value in the surveillance of the diseases. This research was conducted to evaluate serum immunoglobulin levels in women with ovarian cancer and to assess their role in disease process. The present study is composed of 85 women (mean age = 62.03±12.4 yrs) with clinically and pathologically confirmed ovarian cancer and 65 healthy females as a control group (mean age = 61±12.1 yrs). ELISA test was achieved for the determination of serum [IgG, IgA, IgM]. The findings of current study illustrated significant (P=0.001) increase in serum IgG, IgA, and IgM levels as compared to controls. Analyzing serum immunoglobulins levels might assist in identify patients with a weak prediction, the elevation of serum immunoglobulins can be considered as an indication for disease status

Alteration of Serum Immunoglobulin Levels in Woman with Ovarian Cancer

Ovarian cancer has a high mortality and delayed diagnosis. Several immunological alterations take place during ovarian carcinogenesis, and can be of value in the surveillance of the diseases. This research was conducted to evaluate serum immunoglobulin levels in women with ovarian cancer and to assess their role in disease process. The present study is composed of 85 women (mean age = 62.03±12.4 yrs) with clinically and pathologically confirmed ovarian cancer and 65 healthy females as a control group (mean age = 61±12.1 yrs). ELISA test was achieved for the determination of serum [IgG, IgA, IgM]. The findings of current study illustrated significant (P=0.001) increase in serum IgG, IgA, and IgM levels as compared to controls. Analyzing serum immunoglobulins levels might assist in identify patients with a weak prediction, the elevation of serum immunoglobulins can be considered as an indication for disease status.&#x0D;  </jats:p

The Role of procollagen type 1 amino-terminal propertied (P1NP) Cytochrome P450 (CYPs) and Osteoprotegerin (OPG) as Potential Bone function markers in Prostate Cancer Bone Metastasis

Abstract Background: Procollagen type I amino-terminal propeptide (PINP) is often present during osteoblast development and could be a biomarker of early bone development. Osteoprotegerin (OPG) may protect tumor cells from apoptosis. Cytochrome P450 enzymes help tumor development and treatment (CYPs). Cytochrome P450 activates and deactivates anticancer drugs and procarcinogens. Objective: The study examined the amounts of a diagnostic marker of bone formation, the amino terminal propeptide of type I procollagen (PINP), Osteoprotegerin (OPG), and P450, in prostate cancer patients at different stages and its ability to detect osteoblastic metastases. Methods: ELISA was used to measure PINP, OPG, and P450 levels in 30 prostate cancer patients. (n = 32) and healthy men’s serum (n = 36). Results: Prostate cancer patients had higher blood levels of PINP, OPG, and P450 than healthy persons (301.3±134.9, 980±467.2, and 84.2±28.4 pg/mL, respectively). Compared to I+II prostate cancer patients, III+IV patients showed higher serum PINP, OPG, and P450 levels (P 0.001). OPG, P450, and PINP had statistically significant Area under the ROC curve (0.9467, P= 0.0001, 0.91, P= 0.0001, and 0.6977, P= 0.4035) in prostate cancer patients. Conclusions: Metastatic prostate cancer patients had greater PINP, OPG, and P450 levels, according to our findings. PINP, OPG, and P450 levels may affect prostate cancer progression. These findings imply that serum PINP, OPG, and P450 levels may predict and diagnose prostate cancer.</jats:p