An Immunohistochemical Study Of Survivin Expression In Normal And In Transformed Cells [QH585.2. I82 2006 f rb].

Survivin is a new member of the inhibitors of apoptosis protein (IAP) family,selectively over-expressed in common human cancers but not in normal adult tissues. It is also expressed in cancer cell lines. Survivin merupakan ahli baru dalam keluarga protin perencat apoptosis, secara terpilih diekspres secara berlebihan dalam kebanyakan kanser tetapi tidak di dalam tisu dewasa normal. Ia juga diekspres di dalam rangkaian sel-sel kanser

An Immunohistochemical, Study Of Survivin Expression In Normal And In Transformed Cells

Survivin merupakan ahli barn dalam keluarga protin perencat apoptosis, secara terpilih diekspres secara berlebihan dalam kebanyakan kanser tetapi tidak di dalam tisu dewasa nonnaL Ia juga diekspres di dalarn rangkaian sel-sel kanser. Kajian ini dilakukan bagi mengkaji secara asas dan klinikal tentang survivin di dalam sel normal- dan sel tertransfonnasi. Amab telah diirnunkan dengan sintetik oligopeptida, jujukan asid amino, MGAPTLPP A WQP dan KEFEET AKKVRRAIEQLAAMD daripada molekul survivin. Antibodi serum ditulenkan dengan ammonium sulfat dan asid caprilik dan speksifikasinya telah disahkan dengan teknik imrnunoblot dan ujian penyerapan awal terhadap tisu positif survivin dan sintetik oligopeptidasurvivin. Antibodi ini telah digunakan untuk -rnengesan survivin di dalam sel nonnal dan sel tertransfonnasi menggunakan kaedah imunohistokirnia pada hirisan tisu fonnalin-paraffm dan diukur menggunakan kaedah sistern pengskoran piawai dan analisis optimasi. Survivin is a new member of the inhibitors of apoptosis protein (IAP) family, selectively over-expressed in common human cancers but not in normal adult tissues. It is also expressed in cancer cell lines. The study was performed generally to investigate the basic and clinical roles of survivin in normal and transformed cells. Rabbits were immunized with two synthetic oligopeptides, MGAPTLPP A WQP and KEFEET AKKVRRAIEQLAAMD amino acids sequences of the survivin molecule. Serum antibodies were purified by ammonium sulphate and caprilic acid and their specificities were confirmed by immunoblo~ing and pre-absorption tests against survivin positive tissues or synthetic survivin oligopeptides. These antibodies were used to detect survivin in normal and transformed cells by immunohistochemistry in formalin-fixed paraffin embedded tissue sections, evaluated by a standard scoring system and chequerboard analysis

An Immunohistochemical Study Of Survivin Expression In Normal And In Transformed Cells

Survivin is a new member of the inhibitors of apoptosis protein (IAP) family, selectively over-expressed in common human cancers but not in normal adult tissues. It is also expressed in cancer cell lines. The study was performed generally to investigate the basic and clinical roles of survivin in normal and transformed cells. Rabbits were immunized with two synthetic oligopeptides, MGAPTLPPAWQP and KEFEETAKKVRRAIEQLAAMD amino acids sequences of the survivin molecule

Micronutrient levels among aborigines in Pahang and Perak

Micronutrients are essential component of human life. Only small amounts are required for normal growth. The objective of this study was to assess micronutrient status among aborigines living in Sg Siput (U), Perak and Kuala Lipis , Pahang. Our study showed that serum selenium, manganese, magnesium and zinc levels for aborigines were 0.517+0.243pmol/l (n=442), 0.221+0.016pg/l (n=457), 1.178+0.221 mmol/l (n=455) and 15.8472+1.348pmol/ (n=453) respectively. In general, serum selenium and manganese among aborigines were deficient compared to the levels of other population, whilst their serum magnesium and zinc were normal when compared to others population

Expression of human mammaglobin and clinicopathologic correlations in breast cancer: The findings in Malaysia

Background: Human mammaglobin (hMAG) is a secreted protein which has been detected in breast epithelial cells of mammary glands and has been used as a specific marker for breast cancer. Objectives: This study aims at studying the hMAG expression and identifying the significant predictors of hMAG expression in breast cancer tissues. Materials and Methods: The tissue samples were obtained from two major teaching hospitals in the country. They were examined by immunohistochemistry (IHC) and the hMAG expression was evaluated using an established scoring system. Results: Out of 84 breast cancer tissue samples, hMAG was expressed in 50 samples (59.6%). The expression of hMAG was found to be increased with cancer grade. The output of logistic regression model showed that hMAG was overexpressed in breast cancer samples from the first hospital (P = 0.014), but not with those from the second hospital. Conclusions: It can be concluded that hMAG may serve in the diagnosis and the assessment of progression with the increased cancer grade. The dominance in hMAG expression in samples from HUSM may correlate with ethnic, environmental or genetic factors