Associations between genetic variants of the POU1F1 gene and production traits in Saanen goats

WOS: 000466883100001This study was conducted to determine the polymorphisms of the POU1F1 gene and their relationships with milk yield and components, litter size, birth weight, and weaning weight in goats. For this purpose, a total of 108 Saanen goats from two different farms (Bornova and Manisa) were used as animal materials. Polymorphisms at the exon 6 and the 3' flanking region of the POU1F1 gene were determined by using PCR-RFLP with PstI and AluI restriction enzymes and DNA sequencing analyses. Two alleles and three genotypes were identified by AluI or PstI digestions of the POU1F1 gene. The genotypes frequencies of TT, TC, and CC were 64.8 %, 31.5 % and 3.7 % for the PstI locus; 54.6 %, 31.5 % and 13.9 % for the AluI locus, respectively. T allele frequencies (0.56 and 0.88 for the AluI locus, 0.80 and 0.81 for the PstI locus, respectively) were predominant in both loci at the Bornova and Manisa farms. In terms of POU1F1-AluI and POU1F1-PstI loci, two populations were found to be in Hardy-Weinberg equilibrium. In the POU1F1-Alullocus, significant associations were found between genotypes and lactation milk yield and litter size. Similarly, a significant relationship between genotypes and birth weight in the POU1F1-PstI locus (p < 0.05) was determined. The TC and CC genotypes were observed to be higher than the TT genotype for lactation milk yield and litter size at the POU1F1-AluI locus. Birth weight was found to be higher in animals that have the CC genotype at the POU1F1-PstI locus. In conclusion, the POU1F1 gene can be used as a molecular marker for economic features like reproduction, growth, milk content and yield in Saanen goats.Priority Areas National Scholarship Program [TUBITAK-BIDEB 2211-C]This paper is a summary of the PhD thesis of the first author, who was supported by TUBITAK-BIDEB 2211-C Priority Areas National Scholarship Program for PhD Students

Associations between genetic variants of the insulin-like growth factor I (IGF-I) gene and growth traits in European sea bass (Dicentrarchus labrax, L.)

OZCAN GOKCEK, EMEL/0000-0001-6566-7680; ISIK, RAZIYE/0000-0003-2982-6562WOS: 000518425400001PubMed: 32140978The IGF-I is a significant peptide hormone to cell growth, development, reproduction, and metabolism of teleost fish. This study was conducted to investigate the IGF-I gene polymorphism and to reveal the association between the genotypes of IGF-I gene and growth traits in 80 harvest size European sea bass (Dicentrarchus labrax, L.) reared in Izmir region of Turkey. the 821 bp long exon 1 and 5' UTR regions of IGF-I gene were amplified, and PCR products were analyzed via DNA sequencing method. Two novel single nucleotide polymorphisms (SNPs) such as g.46672A > G and g.46749C > T in the 5' UTR region of IGF-I gene in the European sea bass were detected for the first time with this study. Associations between the genotypes of IGF-I g.46749C > T locus and body weight were found statistically significant (p T and g.46672A > G loci were found significantly associated with total length (p < 0.05). However, both loci did not show significant associations with body width. the studied gene region sequences were entered to NCBI GenBank database with the accession numbers: MK621680-MK621682. Thus, these polymorphisms have a significant effect on the body weight and total length which could be useful for sea bass selection and breeding for marker-assisted selection (MAS) program

Genetic Variation of Insulin-like Growth Factor II (IGF-II) Gene and its Associations with Growth Traits in European Sea bass (Dicentrarchus labrax)

ISIK, RAZIYE/0000-0003-2982-6562WOS: 000531553700004Insulin-like growth factor II (IGF-II) is a peptide hormone which plays a crucial role in the growth and metabolic regulations of vertebrates. in this study, we identified single nucleotide polymorphisms on IGF-II gene in the European sea bass (Dicentrarchus labrax) and revealed the potential association between the genotypes of IGF-II gene and growth traits in 150 European sea bass. the polymorphisms were determined using DNA sequencing and PCR-RFLP methods in two different regions of IGF-II gene. An AT indel and two SNPs as g.5127711C>G and g.5127731G>T in the intron 2 region, and one SNP as g.5129188C>T in the intron 3 region of IGF-II gene in the European sea bass were reported for the first time with this study. Two different genotypes (GG, TG) were observed and the G allele was predominant (65%) in the IGF-II-Ndel locus. Associations between the genotypes of IGF-11-Ndel locus and body weight and total length were statistically significant (P<0.05). Body weight and total length in each group were higher for GG genotype of IGF-II-Ndel locus (p<0.05). in conclusion, the IGF-II-Ndel locus had a significant effect on the body weight and total length which could be useful for sea bass selection and breeding for marker-assisted selection program.Scientific Research Projects Coordination Unit of Ege University [16-SUF-025]The funding of the current research was supported by Scientific Research Projects Coordination Unit of Ege University (Project No: 16-SUF-025). We thank Evren Koban Bastanlar for her scientific contributions during writing stage of this article