LXR agonist increases apoE secretion from HepG2 spheroid, together with an increased production of VLDL and apoE-rich large HDL

<p>Abstract</p> <p>Background</p> <p>The physiological regulation of hepatic apoE gene has not been clarified, although the expression of apoE in adipocytes and macrophages has been known to be regulated by LXR.</p> <p>Methods and Results</p> <p>We investigated the effect of TO901317, a LXR agonist, on hepatic apoE production utilizing HepG2 cells cultured in spheroid form, known to be more differentiated than HepG2 cells in monolayer culture. Spheroid HepG2 cells were prepared in alginate-beads. The secretions of albumin, apoE and apoA-I from spheroid HepG2 cells were significantly increased compared to those from monolayer HepG2 cells, and these increases were accompanied by increased mRNA levels of apoE and apoA-I. Several nuclear receptors including LXRα also became abundant in nuclear fractions in spheroid HepG2 cells. Treatment with TO901317 significantly increased apoE protein secretion from spheroid HepG2 cells, which was also associated with the increased expression of apoE mRNA. Separation of the media with FPLC revealed that the production of apoE-rich large HDL particles were enhanced even at low concentration of TO901317, and at higher concentration of TO901317, production of VLDL particles increased as well.</p> <p>Conclusions</p> <p>LXR activation enhanced the expression of hepatic apoE, together with the alteration of lipoprotein particles produced from the differentiated hepatocyte-derived cells. HepG2 spheroids might serve as a good model of well-differentiated human hepatocytes for future investigations of hepatic lipid metabolism.</p

Pseudogap of metallic layered nickelate R2-xSrxNiO4 (R=Nd, Eu) crystals measured using angle-resolved photoemission spectroscopy

We have investigated charge dynamics and electronic structures for single crystals of metallic layered nickelates, R2-xSrxNiO4 (R=Nd, Eu), isostructural to La2-xSrxCuO4. Angle-resolved photoemission spectroscopy on the barely-metallic Eu0.9Sr1.1NiO4 (R=Eu, x=1.1) has revealed a large hole surface of x2-y2 character with a high-energy pseudogap of the same symmetry and comparable magnitude with those of underdoped (x<0.1) cuprates, although the antiferromagnetic interactions are one order of magnitude smaller. This finding strongly indicates that the momentum-dependent pseudogap feature in the layered nickelate arises from the real-space charge correlation.Comment: 4 pages, 4 figures. Accepted in Physical Review Letter

Quantum phase transition in a minimal model for the Kondo effect in a Josephson junction

We propose a minimal model for the Josephson current through a quantum dot in a Kondo regime. We start with the model that consists of an Anderson impurity connected to two superconducting (SC) leads with the gaps $\Delta_{\alpha}=|\Delta_{\alpha}| e^{i \theta_{\alpha}}$, where $\alpha = L, R$ for the lead at left and right. We show that, when one of the SC gaps is much larger than the others $|\Delta_L| \gg |\Delta_R|$, the starting model can be mapped exactly onto the single-channel model, which consists of the right lead of $\Delta_R$ and the Anderson impurity with an extra onsite SC gap of $\Delta_d \equiv \Gamma_L e^{i \theta_L}$. Here $\theta_L$ and $\Gamma_L$ are defined with respect to the starting model, and $\Gamma_L$ is the level width due to the coupling with the left lead. Based on this simplified model, we study the ground-state properties for the asymmetric gap, $|\Delta_L| \gg |\Delta_R|$, using the numerical renormalization group (NRG) method. The results show that the phase difference of the SC gaps $\phi \equiv \theta_R -\theta_L$, which induces the Josephson current, disturbs the screening of the local moment to destabilize the singlet ground state typical of the Kondo system. It can also drive the quantum phase transition to a magnetic doublet ground state, and at the critical point the Josephson current shows a discontinuous change. The asymmetry of the two SC gaps causes a re-entrant magnetic phase, in which the in-gap bound state lies close to the Fermi level.Comment: 23 pages, 13 figures, typos are correcte

Binegativity and geometry of entangled states in two qubits

We prove that the binegativity is always positive for any two-qubit state. As a result, as suggested by the previous works, the asymptotic relative entropy of entanglement in two qubits does not exceed the Rains bound, and the PPT-entanglement cost for any two-qubit state is determined to be the logarithmic negativity of the state. Further, the proof reveals some geometrical characteristics of the entangled states, and shows that the partial transposition can give another separable approximation of the entangled state in two qubits.Comment: 5 pages, 3 figures. I made the proof more transparen

Evaluation of the Imaging Process for a Novel Subtraction Method Using Apparent Diffusion Coefficient Values

Diffusion-weighted imaging may be used to obtain the apparent diffusion coefficient (ADC), which aids the diagnosis of cerebral infarction and tumors. An ADC reflects elements of free diffusion. Diffusion kurtosis imaging (DKI) has attracted attention as a restricted diffusion imaging technique. The ADC subtraction method (ASM) was developed to visualize restricted diffusion with high resolution by using two ADC maps taken with different diffusion times. We conducted the present study to provide a bridge between the reported basic ASM research and clinical research. We developed new imaging software for clinical use and evaluated its performance herein. This software performs the imaging process automatically and continuously at the pixel level, using ImageJ software. The new software uses a macro or a plugin which is compatible with various operating systems via a Java Virtual Machine. We tested the new imaging software’s performance by using a Jurkat cell bio-phantom, and the statistical evaluation of the performance clarified that the ASM values of 99.98% of the pixels in the bio-phantom and physiological saline were calculated accurately (p<0.001). The new software may serve as a useful tool for future clinical applications and restricted diffusion imaging research

Classification of qubit entanglement: SL(2,C) versus SU(2) invariance

The role of SU(2) invariants for the classification of multiparty entanglement is discussed and exemplified for the Kempe invariant I_5 of pure three-qubit states. It is found to being an independent invariant only in presence of both W-type entanglement and threetangle. In this case, constant I_5 admits for a wide range of both threetangle and concurrences. Furthermore, the present analysis indicates that an SL^3 orbit of states with equal tangles but continuously varying I_5 must exist. This means that I_5 provides no information on the entanglement in the system in addition to that contained in the tangles (concurrences and threetangle) themselves. Together with the numerical evidence that I_5 is an entanglement monotone this implies that SU(2) invariance or the monotone property are too weak requirements for the characterization and quantification of entanglement for systems of three qubits, and that SL(2,C) invariance is required. This conclusion can be extended to general multipartite systems (including higher local dimension) because the entanglement classes of three-qubit systems appear as subclasses.Comment: 9 pages, 10 figures, revtex

Emergence of non-centrosymmetric topological insulating phase in BiTeI under pressure

The spin-orbit interaction affects the electronic structure of solids in various ways. Topological insulators are one example where the spin-orbit interaction leads the bulk bands to have a non-trivial topology, observable as gapless surface or edge states. Another example is the Rashba effect, which lifts the electron-spin degeneracy as a consequence of spin-orbit interaction under broken inversion symmetry. It is of particular importance to know how these two effects, i.e. the non-trivial topology of electronic states and Rashba spin splitting, interplay with each other. Here we show, through sophisticated first-principles calculations, that BiTeI, a giant bulk Rashba semiconductor, turns into a topological insulator under a reasonable pressure. This material is shown to exhibit several unique features such as, a highly pressure-tunable giant Rashba spin splitting, an unusual pressure-induced quantum phase transition, and more importantly the formation of strikingly different Dirac surface states at opposite sides of the material.Comment: 5 figures are include

Orbital characters of three-dimensional Fermi surfaces in Eu2-xSrxNiO4 as probed by soft-x-ray angle-resolved photoemission spectroscopy

The three-dimensional Fermi surface structure of hole-doped metallic layered nickelate Eu2-xSrxNiO4 (x=1.1), an important counterpart to the isostructural superconducting cuprate La2-xSrxCuO4, is investigated by energy-dependent soft-x-ray angle-resolved photoemission spectroscopy. In addition to a large cylindrical hole Fermi surface analogous to the cuprates, we observe a Gamma-centered 3z2-r2-derived small electron pocket. This finding demonstrates that in the layered nickelate the 3z2-r2 band resides close to the x2-y2 one in energy. The resultant multi-band feature with varying orbital character as revealed may strongly work against the emergence of the high-temperature superconductivity.Comment: 5 pages, 4 figures. Accepted in Physical Review B (Rapid Communication