EWSR1-ATF1融合遺伝子を持つ歯原性明細胞癌細胞株の樹立と性状解析

Objective: Clear cell odontogenic carcinoma (CCOC) is a rare malignant odontogenic tumor (MOT) characterized by sheets and lobules of vacuolated and clear cells. To understand the biology of CCOC, we established a new cell line, CCOC-T, with EWSR1-ATF1 fusion gene from a mandible tumor with distant metastasis and characterized this cell line. Materials and methods: To detect the EWSR1-ATF1 fusion gene, we used three CCOC cases, including the present case, by RT-PCR and FISH analysis. We characterized established CCOC-T cells by checking cell growth, invasion and the expression of odontogenic factors and bone-related factors. Moreover, the gene expression profile of CCOC-T cells was examined by microarray analysis. Results: Histologically, the primary tumor was comprised of cords and nests containing clear and squamoid cells separated by fibrous septa. In addition, ameloblastomatous islands with palisaded peripheral cells were observed, indicating probable odontogenic origin. This tumor expressed the fusion gene EWSR1-ATF1, which underlies the etiology of hyalinizing clear cell carcinoma (HCCC) and potentially that of CCOC. We found a breakpoint in the EWSR1-ATF1 fusion to be the same as that reported in HCCC. Established CCOC-T cells grew extremely slowly, but the cells showed highly invasive activity. Moreover, CCOC-T cells expressed bone-related molecules, odontogenic factors, and epithelial mesenchymal transition (EMT)-related molecules. Conclusion: To the best of our knowledge, this is the first report on the establishment of a CCOC cell line. CCOC-T cells serve as a useful in vitro model for understanding the pathogenesis and nature of MOT

モクタン ノ ビサイ コウゾウ ノ ケイセイ キコウ

京都大学0048新制・課程博士博士(農学)甲第13127号農博第1632号新制||農||942(附属図書館)学位論文||H19||N4253(農学部図書室)UT51-2007-H400京都大学大学院農学研究科森林科学専攻(主査)教授 今村 祐嗣, 教授 矢野 浩之, 教授 杉山 淳司学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDA

<Preliminary>Surface Characterization of Wood Charcoal under Different Carbonization Conditions

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Microstructural study of carbonized wood after cell wall sectioning

Wooden blocks of Japanese cedar (Cryptomeria japonica) were carbonized at 700 and 1,800 degrees C. The microstructure was analyzed by transmission electron microscopy (TEM) and mu-Raman spectroscopy of the inner planes of wood cell walls. The predominant structure was of a turbostratic nature and no heterogeneity was observed originating from the original cell walls. TEM observations of samples carbonized at 1,800 degrees C showed ordered regions in the surface layer of cell walls. This result was supported by polarized mu-Raman analysis. It may be caused by the deposition of carbon compounds volatilized from the cell walls during pulse current heating

Spectroscopic analysis of carbonization behavior of wood, cellulose and lignin

The surface and bulk chemistry of Japanese cedar (Cryptomeria Japonica), cotton cellulose and lignin samples carbonized at 500-1,000 degrees C was investigated by elemental analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and micro-Raman spectrometry. The objective was to link the original wood components to the final carbonized wood microstructures. The carbonized samples show increasing degrees of order from cellulose to wood to lignin. The cellulose component in the wood strongly affects the ordering of polyaromatic carbons in carbonized wood; this ordering is attributed primarily to the difference in ratio between aromatic and aliphatic carbons and to the amount of cross-linking by ether and carboxylic groups up to 500 degrees C

Characterization of sp(2)- and sp(3)-bonded carbon in wood charcoal

Japanese cedar (Cryptomeria japonica) preheated at 700 degrees C was subsequently heated to 1800 degrees C and characterized by electron microscopy, X-ray diffraction, and micro-Raman spectroscopy. The degree of disorder of carbon crystallites and the amount of amorphous phase decreased considerably with an increase in heat treatment temperature to 1400 degrees C, while carbon crystallites clearly developed above this temperature, showing that the microstructure of carbonized wood undergoes drastic changes around 1400 degrees C. Besides showing the bands for sp(2-)bonded carbon, the Raman spectra showed a shoulder near 1100 cm(-1) assigned to sp3-bonded carbon. With an increase of heat treatment temperature, the peak position of the Raman sp(3) band shifted to a lower frequency from 1190 to 1120 cm(-1), which is due to the transformation of sp3-bonded carbon from an amorphous phase to a nanocrystalline phase. These data showed that the microstructure of carbonized wood from 700 degrees to 1800 degrees C consisted of the combination of sp(2-) and sp(3-)bonded carbon, which is probably due to the disordered microstructure of carbonized wood. It is suggested that the sp(3-)bonded carbon is transformed from an amorphous structure to a nanocrystalline structure with the growth of polyaromatic stacks at temperatures above 1400 degrees C