Simple Node Architectures for Connection of Two ROADM Rings Using Hierarchical Optical Path Routing

We propose a wavelength assignment algorithm for full mesh node connectivity of two connected, bidirectional ring networks. It is proved to minimize the necessary number of wavelengths. We also propose novel ring-connecting nodes that can reduce switch size by 90-50% compared to conventional arrangement

p62‐mediated autophagy affects nutrition‐dependent insulin receptor substrate 1 dynamics in 3T3‐L1 preadipocytes

Abstract Aims/Introduction Previous studies have shown that an organism's nutritional status changes the protein levels of insulin receptor substrate 1 (IRS‐1) in a tissue‐specific manner. Although the mechanisms underlying the regulation of IRS‐1 in the nutrient‐rich conditions associated with diabetes and insulin resistance have been well studied, those under nutrient‐poor conditions remain unknown. The aim of the present study was to investigate how IRS‐1 protein levels change depending on the nutritional status of 3T3‐L1 preadipocytes. Materials and Methods 3T3‐L1 preadipocytes were treated with glucose‐, amino acid‐ and serum‐free medium for starvation. IRS‐1 protein levels were detected by western blot. Autophagy activity was observed by western blot and fluorescence microscopy. The effect of autophagy and p62, an adaptor for selective autophagy, on IRS‐1 protein levels under starvation conditions was examined by western blot and immunocytochemistry. Results We showed that the levels of IRS‐1, but not those of insulin receptor and protein kinase B, decreased when starvation activated autophagy. The inhibition of autophagy by chloroquine or autophagy‐related 7 (Atg7) ribonucleic acid interference counteracted the starvation‐induced decrease of IRS‐1. Additionally, Atg7 knockdown increased insulin‐stimulated phosphorylation of protein kinase B under starvation conditions. Furthermore, p62 colocalized with IRS‐1 under starvation conditions, and p62 knockdown counteracted the starvation‐induced degradation of IRS‐1. Conclusions Autophagy through p62 plays an important role in regulating IRS‐1 protein levels in response to nutritional deficiency. The present findings suggest that autophagy might function as energy depletion‐sensing machinery that finely tunes insulin signal transduction

Monolithically Integrated Waveband Selective Switch Using Cyclic AWGs

We propose a novel waveband-selective switch that uses cyclic Arrayed-Waveguide Gratings. The device is fabricated on one chip using Planer Lightwave Circuit technologies. Tests demonstrate that the designed performance is successfully realized

Gazette nationale ou le Moniteur universel

13 octobre 17911791/10/13 (N286)