4,438 research outputs found

    Getting Ahead or Losing Ground: Economic Mobility in America

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    Combines earlier research to present a comprehensive analysis of relative mobility, absolute mobility, and changes in income inequality. Focuses on intergenerational mobility, immigrants, comparisons by gender and race, and the role of education

    How Targeted Are Federal Expenditures on Children? A Kids' Share Analysis of Expenditures by Income in 2009

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    Analyzes the distribution of government spending on children by family income; category, such as health, social services, and education; and program, such as Medicaid; and the extent to which it is targeted to low-income children. Considers implications

    Evaluation of the quality of systematic reviews critiqued by clinical assistants in-training

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    Conduct an overview of systematic reviews (SRs) reviewed by clinical assistants (CAs) in-training. SRs relating to clinical procedures and theoretical concepts, critiqued by CAs were included. Review authors independently screened the results of the requested SRs and evaluated these using the AMSTAR-1 checklist and AMSTAR-2 tool. Differences regarding study outcomes were resolved by consultation. Articles (N=37) submitted to the researcher included 35 reviews published in accredited journals. Of the reviews, only 18 were SRs as stated in their titles and these were of mixed designs and quality; and 17 were either non-structured and biased literature or critical reviews. SR topics reviewed in-training varied; and included temporo-mandibular disorders, implants and implant-supported prosthesis. AMSTAR-1 scores were favourable; scores were low for most SRs using AMSTAR-2, including those with randomized controlled trials only, with the exception of one review that had no randomized controlled trials but fulfilled the critical domain criteria. Students’ misconceptions regarding what constitutes good SRs which are translatable into clinical practice are emphasized, impacting their learning. CAs lack of appraisal skills related to SRs which may influence clinical practices are highlighted

    Authentication of Red Snapper (\u3cem\u3eLutjanus campechanus\u3c/em\u3e) Fillets Using a Combination of Real-time PCR and DNA Barcoding

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    Red snapper (Lutjanus campechanus) is a historically overfished and highly valued species that is commonly substituted with other fish, such as tilapia, rockfish, and other snapper species. The objective of this study was to assess the ability of real-time PCR to be used as a screening tool to rapidly test commercial fillets for the presence of red snapper, followed by species identification of negative samples with DNA barcoding. A total of 24 frozen, fresh, or thawed (previously frozen) fillets labeled as “red snapper” were tested with real-time PCR, along with 54 fillets from fish that are common substitutes for red snapper. Real-time PCR parameters were optimized to reduce cross-reactivity. All samples were also tested with DNA barcoding to confirm the identity of fish species. Among the 78 total samples, 3 were authenticated as red snapper with DNA barcoding and successfully detected with real-time PCR. An additional two samples were initially identified as red snapper with real-time PCR but confirmed negative with DNA barcoding, resulting in a false positive rate of 2.7%. Overall, 39.7% of all samples and 91.7% of “red snapper” samples were mislabeled. Red snapper was substituted with other snapper species, rockfish, sea bream, and mahi-mahi. These results illustrate the ability of real-time PCR to be used as a screening tool and the importance of species confirmation with DNA barcoding. Real-time PCR has the potential to be used as a rapid on-site screening tool for regulatory and industry officials to determine the authenticity of red snapper fillets
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