1 research outputs found
Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma
Author version made available in accordance with publisher policy.Abstract
Background The poor prognosis and rising incidence of esophageal adenocarcinoma highlight the need for improved detection
methods. The potential for circulating microRNAs (miRNAs) as biomarkers in other cancers has been shown, but circulating
miRNAs have not been well characterized in esophageal adenocarcinoma. We investigated whether circulating exosomal
miRNAs have potential to discriminate individuals with esophageal adenocarcinoma from healthy controls and non-dysplastic
Barrett’s esophagus.
Methods Seven hundred fifty-eight miRNAs were profiled in serum circulating exosomes from a cohort of 19 healthy controls,
10 individuals with Barrett’s esophagus, and 18 individuals with locally advanced esophageal adenocarcinoma. MiRNA expression
was assessed using all possible permutations of miRNA ratios per individual. Four hundred eight miRNA ratios were
differentially expressed in individuals with cancer compared to controls and Barrett’s esophagus (Mann-Whitney U test,
P<0.05). The 179/408 ratios discriminated esophageal adenocarcinoma from healthy controls and Barrett’s esophagus (linear
regression, P0.7, P<0.05). A multi-biomarker panel (RNU6-1/miR-
16-5p, miR-25-3p/miR-320a, let-7e-5p/miR-15b-5p, miR-
30a-5p/miR-324-5p, miR-17-5p/miR-194-5p) demonstrated
enhanced specificity and sensitivity (area under ROC=0.99,
95 % CI 0.96–1.0) over single miRNA ratios to distinguish
esophageal adenocarcinoma from controls and Barrett’s
esophagus.
Conclusions This study highlights the potential for serum
exosomal miRNAs as biomarkers for the detection of esophageal
adenocarcinoma