3 research outputs found
Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)
Immunohistochemical and radiological characterization of wound healing in porcine liver after radiofrequency ablation
Background: Radiofrequency ablation (RFA)
is a minimal invasive therapeutic option for patients with
hepatocellular carcinoma or liver metastases. We
investigated RFA-induced cellular changes in the liver of
pigs.
Material and Methods: Healthy pigs (n=18) were
sacrificed between day 0 and 3 months after RFA. The
wound healing process was evaluated by computed
tomography (CT), chromotrope anilinblue (CAB)
staining of large-scale and standard tissue sections.
Immunohistochemistry (IHC) for heat shock protein 70,
Caspase-3, Ki67, Reelin, Vinculin, Vimentin and αSMA was perfomed.
Results: One day after RFA, CAB staining showed
cell damage and massive hyperaemia. All IHC markers
were predominantly expressed at the outer borders of the
lesion, except Reelin, which was mainly detected in
untreated liver regions. By staining for Hsp70, the heat
stress during RFA was monitored, which was most
distinct 1-2 days after RFA. CT revealed decreased
lesion size after one week. Development of a Vimentin
and α-SMA positive fibrotic capsule was observed.
Conclusion: In the early phase signs of cell damage,
apoptosis and proliferation are dominant. Reduced
expression of Reelin suggests a minor role of hepatic
stellate cells in the RFA zone. After one week
myofibroblasts become prominent and contribute to the
development of the fibrotic capsule. This elucidates the
pathophysiology of RFA and could contribute to the
future optimization of RFA procedures