Role of YidC in folding of polytopic membrane proteins

YidC of Echerichia coli, a member of the conserved Alb3/Oxa1/YidC family, is postulated to be important for biogenesis of membrane proteins. Here, we use as a model the lactose permease (LacY), a membrane transport protein with a known three-dimensional structure, to determine whether YidC plays a role in polytopic membrane protein insertion and/or folding. Experiments in vivo and with an in vitro transcription/translation/insertion system demonstrate that YidC is not necessary for insertion per se, but plays an important role in folding of LacY. By using the in vitro system and two monoclonal antibodies directed against conformational epitopes, LacY is shown to bind the antibodies poorly in YidC-depleted membranes. Moreover, LacY also folds improperly in proteoliposomes prepared without YidC. However, when the proteoliposomes are supplemented with purified YidC, LacY folds correctly. The results indicate that YidC plays a primary role in folding of LacY into its final tertiary conformation via an interaction that likely occurs transiently during insertion into the lipid phase of the membrane

Eligibility for social services: social policy development in an international context

The article provides a frame of reference for reviewing the basic processes underlying the development of formal social policy. The bases for the article were provided by: (1) review of the literature in related social services policy development areas; (2) analyses and review of the literature specific to service eligibility; (3) analyses of related socio-economic data from international organizations and registries. Eligibility is proposed as an essential element of social services within the context of citizens’ rights, cultural mores and customs, political processes and economic conditions of the six contributing countries. These data sources were augmented by review of summary narratives of the history and status of social services development.O artigo apresenta um quadro de referência para a revisão dos processos fundamentais subjacentes ao desenvolvimento de políticas sociais formais. O artigo baseia-se em: (1) revisão de literatura das áreas de desenvolvimento das políticas de serviço social; 2) análise e revisão de literatura específica sobre a elegibilidade dos serviços; 3) análise de registos e dados socioeconómicos de organizações internacionais e registos. A elegibilidade é proposta como um elemento essencial dos serviços sociais no contexto dos direitos dos cidadãos, costumes culturais e valores, processos políticos e condições económicas dos seis países contribuintes. Estas fontes de informação foram ampliadas pela revisão de resumos de narrativas da história e estado de desenvolvimento dos serviços sociais.Cet article présente un canevas pour la révision des processus fondamentaux qui sous-tendent la mise en œuvre de politiques sociales formelles, en se basant sur : (1) la révision de la littérature des domaines de développement des politiques de protection sociale ; 2) l’analyse et la révision de la littérature spécifique sur l’éligibilité aux prestations ; 3) l’analyse des fichiers et des données socioéconomiques d’organisations internationales. L’éligibilité est proposée comme un élément essentiel des prestations sociales dans le contexte des droits des citoyens, des habitudes culturelles et des valeurs, des processus politiques et des conditions économiques des six pays contributeurs. Ces sources d’information ont été élargies par la révision de résumés de récits de l’histoire et de l’état de développement des services sociaux.El artículo presenta un marco de referencia para la revisión de los procesos fundamentales subyacentes al desarrollo de políticas sociales formales. El artículo se basa en: (1) revisión de literatura de las áreas de desarrollo de las políticas de servicio social; 2) análisis de la revisión de literatura específica sobre la elegibilidad de los servicios; 3) Análisis de registros y datos socioeconómicos de organizaciones internacionales y registros. La elegibilidad es propuesta como un elemento esencial de los servicios sociales en el contexto de los derechos de los ciudadanos, costumbres culturales  y valores, procesos políticos y condiciones económicas de sus países contribuyentes. Estas fuentes de información fueron ampliadas por la revisión de resúmenes de narrativas de la historia y estado de desarrollo de los servicios sociales

Information technologies in teaching humanitarian disciplines

The use of information technology in teaching humanitarian disciplines contributes to the formation of highly qualified, competitive specialists who can adapt to rapidly changing conditions, possessing a high level of development of critical and creative thinking, ready for professional self-development and self-improvement, for the implementation of reflection, capable of setting goals and systematically achieving them. The work actualizes the importance of information technology in the training of highly qualified competitive specialists capable of implementing professional activities at a high level. The purpose of the article is to analyze the experience of implementing information technologies in teaching humanitarian disciplines. Smart boards, multimedia installations, document cameras, electronic textbooks, electronic educational platforms and others were used as information technologies used in the teaching of the humanities. The study made it possible to reveal the influence of information technologies on the level of formation of students' competencies

The use of narrative as a speech behaviour model

The authors of this paper view narrative as a way for a human to express his or her attitude to the world, finding its presence virtually in every aspect of human relations. Revealing the essence of a narrative approach, the authors refer to the works by J. Brockmeier, R. Harré, A. Schütz, J.F. Lyotard, J. Bruner who analyzed the essence of narrative in their studies. The structure, characteristics, forms and functions of narrative as a type of discursive reality are analyzed. Particular attention is paid to personal narrative as a way to reflect acquired experience. The authors underline the use of narrative techniques in many knowledge areas: psychology, sociology, linguistics, philosophy, and psychiatry. Analyzing pedagogical aspects of narrative, the authors mention the use of metaphors as a means of influencing the audience. The authors pay a special attention to the practicability of narrative teaching methods, namely a narrative game as one of the methods of active learning in an educational process.peer-reviewe

Самостоятельная работа студентов в профессиональных учебных заведениях

The reform of education in Russia has led to emergence of new learning pattern. Today, among numerous changes that have occurred in competence-based approach context, we note tendencies towards changes in teacher’s role in a student’s life, reduction in classroom hours and increase in the share of students’ independent work. In order to preserve and increase effectiveness of student learning, educational institutions should aim at finding new ways to use classroom hours to organize students' independent work. Insufficient knowledge of this topic actualizes the process of its further development. The purpose of our research was to verify the structure of independent work developed by the authors at a technical school in order to increase student training level. For this, the authors conducted an experiment in which two groups of second-year students of 25 people each took part. The results of the differentiated classification of two groups in the course "Engineering Graphics" were compared. The first group of students studied according to the structure available in the college, the second - with the help of the pattern developed by the authors. As a result, it was found that the second group has a higher level of training. The results of the experiment allowed us to conclude that the structure of independent work developed by the authors is very effective and its implementation will allow achieving positive results in students' competences development and in future graduates training.La reforma de la educación en Rusia ha llevado a la aparición de un nuevo patrón de aprendizaje. Hoy, entre los numerosos cambios que se han producido en el contexto del enfoque basado en la competencia, observamos tendencias hacia cambios en el papel del maestro en la vida de un estudiante, reducción en las horas de clase y aumento en la parte del trabajo independiente de los estudiantes. Para preservar y aumentar la efectividad del aprendizaje de los estudiantes, las instituciones educativas deben apuntar a encontrar nuevas formas de usar las horas de clase para organizar el trabajo independiente de los estudiantes. El conocimiento insuficiente de este tema actualiza el proceso de su desarrollo posterior. El propósito de nuestra investigación fue verificar la estructura del trabajo independiente desarrollado por los autores en una escuela técnica para aumentar el nivel de capacitación de los estudiantes. Para ello, los autores realizaron un experimento en el que participaron dos grupos de estudiantes de segundo año de 25 personas cada uno. Se compararon los resultados de la clasificación diferenciada de dos grupos en el curso "Gráficos de ingeniería". El primer grupo de estudiantes estudió de acuerdo con la estructura disponible en la universidad, el segundo, con la ayuda del patrón desarrollado por los autores. Como resultado, se encontró que el segundo grupo tiene un mayor nivel de entrenamiento. Los resultados del experimento nos permitieron concluir que la estructura del trabajo independiente desarrollado por los autores es muy efectiva y su implementación permitirá lograr resultados positivos en el desarrollo de competencias de los estudiantes y en la formación de futuros graduados.Реформа образования в России привела к появлению новой модели обучения. Сегодня среди многочисленных изменений, произошедших в контексте компетентностного подхода, мы отмечаем тенденции к изменению роли педагога в жизни студента, сокращению аудиторных часов и увеличению доли самостоятельной работы студентов. Чтобы сохранить и повысить эффективность обучения студентов, учебные заведения должны стремиться найти новые способы использования аудиторных часов для организации самостоятельной работы студентов. Недостаточная разработка этой темы актуализирует процесс ее дальнейшего развития. Целью нашего исследования была проверка структуры самостоятельной работы, разработанной авторами в техникуме с целью повышения уровня подготовки студентов. Для этого авторы провели эксперимент, в котором приняли участие две группы студентов второго курса по 25 человек в каждой. Результаты дифференцированной классификации двух групп по курсу «Инженерная графика» сравнивались. Первая группа студентов обучалась по структуре, имеющейся в колледже, вторая - по схеме, разработанной авторами. В результате выяснилось, что вторая группа имеет более высокий уровень подготовки. Результаты эксперимента позволили сделать вывод о том, что разработанная авторами структура самостоятельной работы имеет высокую результативность, а ее реализация позволит достичь положительных результатов в развитии компетенций студентов и в подготовке будущих выпускников

A STUDY OF SOCIAL ADVERTS WITH RESPECT TO THE IMAGE OF MUSLIM REFUGEES IN SOCIETY

Purpose: The article deals with the problem of changes in social values made by the influence of refugee culture and mind on the British society, which is widely known to be very stable and unchangeable via comparative qualitative research methods. Methodology: The qualitative research adopted an analytical-comparative method. Data were collected through observation, interviews and questionnaires. Main Findings: As a result, the value-ignored actions from the Love letter are the most diverse in value types and in the number of uses in the text. In conclusion, when considering the verb value-oriented expressions, it is noted that there are much more positive actions than negative ones. Applications: This article can be used by regional schools, cultural organizations and universities. Novelty/Originality: The crisis of ideology in the field of morality in the modern social situation related to the problems of immigrants has been studied

Utilization of an In Vivo Reporter for High Throughput Identification of Branched Small Molecule Regulators of Hypoxic Adaptation

SummarySmall molecules inhibiting hypoxia inducible factor (HIF) prolyl hydroxylases (PHDs) are the focus of drug development efforts directed toward the treatment of ischemia and metabolic imbalance. A cell-based reporter produced by fusing HIF-1α oxygen degradable domain (ODD) to luciferase was shown to work as a capture assay monitoring stability of the overexpressed luciferase-labeled HIF PHD substrate under conditions more physiological than in vitro test tubes. High throughput screening identified novel catechol and oxyquinoline pharmacophores with a “branching motif” immediately adjacent to a Fe-binding motif that fits selectively into the HIF PHD active site in in silico models. In accord with their structure-activity relationship in the primary screen, the best “hits” stabilize HIF1α, upregulate known HIF target genes in a human neuronal line, and exert neuroprotective effects in established model of oxidative stress in cortical neurons

A Model of Technical University Students’ Creative-Project Activities’ Systemic Commitment to Their Self-Development and the Experimental Verification of Its Effectiveness

The urgency of the need to develop a model of the systemic commitment of creative-project activity of technical university students to their self-development is due to the fact that the reserve possibilities of this type of teaching, research and professional activities from the perspective of the systemic commitment to self-development of the students’ competitiveness remain insufficiently investigated in the theory and practice of teacher education. In this regard, the article presents the main components of the model of systemic commitment of the creative-project activity of technical university students to their self-development, as well as the results for its implementation in practice of higher professional education. The presented components of the model in the article (objectives, contents, methods, learning and educational environment) are working for the ultimate goal − for self-development of professional creativity, self-development of the competitiveness of a student as a prospective specialist. The results of the article can be used by administrators and faculty members of higher education institutions. DOI: 10.5901/mjss.2015.v6n2s3p12