The ATP-dependent generation of membrane potential by sub-bacterial vesicles from the marine bacterium, Vibrio alginolyticus

AbstractAddition of ATP leads to the accumulation of the permeant anion PCB− by sub-bacterial vesicles from Vibrio alginolyticus. This accumulation is caused by Δψ generation by ATPase, the effect being inhibited by CCCP, gramicidin D and DCCD. Δψ values may be increased by incubation of sub-bacterial vesicles at room temperature and with the protein fraction isolated according to Beechey et al. [(1975) Biochem. J. 148, 533–537] from another portion of the sub-bacterial vesicles. Δψ generation is observable only in the presence of Mg2+ at high concentrations (optimum ≈ 30 mM). Proceeding from experimental data we assume that Mg2+ reduces passive H+ conductivity of the vesicle membranes. Thus, a Δψ-generating ATPase has been shown for the first time in V. alginolyticus membranes

Time Attitudes in Contemporary Men and Women

Introduction. This paper investigates predictors of time attitudes in men and women aged 25–45 years. A theoretical analysis concentrates on the concept of time in the context of classical, non-classical, and post-non-classical ideals of rationality. In the process of individuals’ interaction with objects, time is born as a qualitatively new reality that is reducible neither to the subjective nor to the objective perspective. The psychological category of time is interpreted within the framework of post-non-classical approach that is capable of solving that contradiction. Methods. The survey method was employed in the study for data collection. Mathematical and statistical analyses included Student’s t-test for independent samples and step-wise regression analysis. Results. The transspective analysis of the problem of time in psychology enabled authors to describe its dynamics at classical, non-classical, and post-non-classical stages. The study examined time attitudes in men and women and determined their predictors in the context of gender differences. Discussion. This is the first study that examines the predictors of time attitudes in men and women. Its original findings suggest that there is a dependency between time attitudes and parameters of personal and subjective functioning, including self-actualization, psychological well-being, coping strategies, and psychological defenses. Not so much certain values, as the subsystems of values belonging to various aspects of life (family, hobby, profession, household, etc.) participate in the formation process of time attitudes in men and women. Personal values determine the specific character of regulation of the time of life in women and men. Coping strategies are important for personal organization of time. The study showed the differences in interrelationships between value choices and time attitudes in men and women

Самостоятельная работа студентов в профессиональных учебных заведениях

The reform of education in Russia has led to emergence of new learning pattern. Today, among numerous changes that have occurred in competence-based approach context, we note tendencies towards changes in teacher’s role in a student’s life, reduction in classroom hours and increase in the share of students’ independent work. In order to preserve and increase effectiveness of student learning, educational institutions should aim at finding new ways to use classroom hours to organize students' independent work. Insufficient knowledge of this topic actualizes the process of its further development. The purpose of our research was to verify the structure of independent work developed by the authors at a technical school in order to increase student training level. For this, the authors conducted an experiment in which two groups of second-year students of 25 people each took part. The results of the differentiated classification of two groups in the course "Engineering Graphics" were compared. The first group of students studied according to the structure available in the college, the second - with the help of the pattern developed by the authors. As a result, it was found that the second group has a higher level of training. The results of the experiment allowed us to conclude that the structure of independent work developed by the authors is very effective and its implementation will allow achieving positive results in students' competences development and in future graduates training.La reforma de la educación en Rusia ha llevado a la aparición de un nuevo patrón de aprendizaje. Hoy, entre los numerosos cambios que se han producido en el contexto del enfoque basado en la competencia, observamos tendencias hacia cambios en el papel del maestro en la vida de un estudiante, reducción en las horas de clase y aumento en la parte del trabajo independiente de los estudiantes. Para preservar y aumentar la efectividad del aprendizaje de los estudiantes, las instituciones educativas deben apuntar a encontrar nuevas formas de usar las horas de clase para organizar el trabajo independiente de los estudiantes. El conocimiento insuficiente de este tema actualiza el proceso de su desarrollo posterior. El propósito de nuestra investigación fue verificar la estructura del trabajo independiente desarrollado por los autores en una escuela técnica para aumentar el nivel de capacitación de los estudiantes. Para ello, los autores realizaron un experimento en el que participaron dos grupos de estudiantes de segundo año de 25 personas cada uno. Se compararon los resultados de la clasificación diferenciada de dos grupos en el curso "Gráficos de ingeniería". El primer grupo de estudiantes estudió de acuerdo con la estructura disponible en la universidad, el segundo, con la ayuda del patrón desarrollado por los autores. Como resultado, se encontró que el segundo grupo tiene un mayor nivel de entrenamiento. Los resultados del experimento nos permitieron concluir que la estructura del trabajo independiente desarrollado por los autores es muy efectiva y su implementación permitirá lograr resultados positivos en el desarrollo de competencias de los estudiantes y en la formación de futuros graduados.Реформа образования в России привела к появлению новой модели обучения. Сегодня среди многочисленных изменений, произошедших в контексте компетентностного подхода, мы отмечаем тенденции к изменению роли педагога в жизни студента, сокращению аудиторных часов и увеличению доли самостоятельной работы студентов. Чтобы сохранить и повысить эффективность обучения студентов, учебные заведения должны стремиться найти новые способы использования аудиторных часов для организации самостоятельной работы студентов. Недостаточная разработка этой темы актуализирует процесс ее дальнейшего развития. Целью нашего исследования была проверка структуры самостоятельной работы, разработанной авторами в техникуме с целью повышения уровня подготовки студентов. Для этого авторы провели эксперимент, в котором приняли участие две группы студентов второго курса по 25 человек в каждой. Результаты дифференцированной классификации двух групп по курсу «Инженерная графика» сравнивались. Первая группа студентов обучалась по структуре, имеющейся в колледже, вторая - по схеме, разработанной авторами. В результате выяснилось, что вторая группа имеет более высокий уровень подготовки. Результаты эксперимента позволили сделать вывод о том, что разработанная авторами структура самостоятельной работы имеет высокую результативность, а ее реализация позволит достичь положительных результатов в развитии компетенций студентов и в подготовке будущих выпускников

Eligibility for social services: social policy development in an international context

The article provides a frame of reference for reviewing the basic processes underlying the development of formal social policy. The bases for the article were provided by: (1) review of the literature in related social services policy development areas; (2) analyses and review of the literature specific to service eligibility; (3) analyses of related socio-economic data from international organizations and registries. Eligibility is proposed as an essential element of social services within the context of citizens’ rights, cultural mores and customs, political processes and economic conditions of the six contributing countries. These data sources were augmented by review of summary narratives of the history and status of social services development.O artigo apresenta um quadro de referência para a revisão dos processos fundamentais subjacentes ao desenvolvimento de políticas sociais formais. O artigo baseia-se em: (1) revisão de literatura das áreas de desenvolvimento das políticas de serviço social; 2) análise e revisão de literatura específica sobre a elegibilidade dos serviços; 3) análise de registos e dados socioeconómicos de organizações internacionais e registos. A elegibilidade é proposta como um elemento essencial dos serviços sociais no contexto dos direitos dos cidadãos, costumes culturais e valores, processos políticos e condições económicas dos seis países contribuintes. Estas fontes de informação foram ampliadas pela revisão de resumos de narrativas da história e estado de desenvolvimento dos serviços sociais.Cet article présente un canevas pour la révision des processus fondamentaux qui sous-tendent la mise en œuvre de politiques sociales formelles, en se basant sur : (1) la révision de la littérature des domaines de développement des politiques de protection sociale ; 2) l’analyse et la révision de la littérature spécifique sur l’éligibilité aux prestations ; 3) l’analyse des fichiers et des données socioéconomiques d’organisations internationales. L’éligibilité est proposée comme un élément essentiel des prestations sociales dans le contexte des droits des citoyens, des habitudes culturelles et des valeurs, des processus politiques et des conditions économiques des six pays contributeurs. Ces sources d’information ont été élargies par la révision de résumés de récits de l’histoire et de l’état de développement des services sociaux.El artículo presenta un marco de referencia para la revisión de los procesos fundamentales subyacentes al desarrollo de políticas sociales formales. El artículo se basa en: (1) revisión de literatura de las áreas de desarrollo de las políticas de servicio social; 2) análisis de la revisión de literatura específica sobre la elegibilidad de los servicios; 3) Análisis de registros y datos socioeconómicos de organizaciones internacionales y registros. La elegibilidad es propuesta como un elemento esencial de los servicios sociales en el contexto de los derechos de los ciudadanos, costumbres culturales  y valores, procesos políticos y condiciones económicas de sus países contribuyentes. Estas fuentes de información fueron ampliadas por la revisión de resúmenes de narrativas de la historia y estado de desarrollo de los servicios sociales

Information technologies in teaching humanitarian disciplines

The use of information technology in teaching humanitarian disciplines contributes to the formation of highly qualified, competitive specialists who can adapt to rapidly changing conditions, possessing a high level of development of critical and creative thinking, ready for professional self-development and self-improvement, for the implementation of reflection, capable of setting goals and systematically achieving them. The work actualizes the importance of information technology in the training of highly qualified competitive specialists capable of implementing professional activities at a high level. The purpose of the article is to analyze the experience of implementing information technologies in teaching humanitarian disciplines. Smart boards, multimedia installations, document cameras, electronic textbooks, electronic educational platforms and others were used as information technologies used in the teaching of the humanities. The study made it possible to reveal the influence of information technologies on the level of formation of students' competencies

A STUDY OF SOCIAL ADVERTS WITH RESPECT TO THE IMAGE OF MUSLIM REFUGEES IN SOCIETY

Purpose: The article deals with the problem of changes in social values made by the influence of refugee culture and mind on the British society, which is widely known to be very stable and unchangeable via comparative qualitative research methods. Methodology: The qualitative research adopted an analytical-comparative method. Data were collected through observation, interviews and questionnaires. Main Findings: As a result, the value-ignored actions from the Love letter are the most diverse in value types and in the number of uses in the text. In conclusion, when considering the verb value-oriented expressions, it is noted that there are much more positive actions than negative ones. Applications: This article can be used by regional schools, cultural organizations and universities. Novelty/Originality: The crisis of ideology in the field of morality in the modern social situation related to the problems of immigrants has been studied

The use of narrative as a speech behaviour model

The authors of this paper view narrative as a way for a human to express his or her attitude to the world, finding its presence virtually in every aspect of human relations. Revealing the essence of a narrative approach, the authors refer to the works by J. Brockmeier, R. Harré, A. Schütz, J.F. Lyotard, J. Bruner who analyzed the essence of narrative in their studies. The structure, characteristics, forms and functions of narrative as a type of discursive reality are analyzed. Particular attention is paid to personal narrative as a way to reflect acquired experience. The authors underline the use of narrative techniques in many knowledge areas: psychology, sociology, linguistics, philosophy, and psychiatry. Analyzing pedagogical aspects of narrative, the authors mention the use of metaphors as a means of influencing the audience. The authors pay a special attention to the practicability of narrative teaching methods, namely a narrative game as one of the methods of active learning in an educational process.peer-reviewe

SEMANTIC FEATURES OF THE PHRASEOLOGICAL UNITS WITH THE COMPONENT LIGHT THE WITHIN ARTISTIC DISCOURSE

Purpose: To conduct lexical and semantic analysis on the concept light in the artistic discourse of postmodern fiction. Methodology: In this research, comparative method, search for synonyms, continuous sampling and seminal analysis are used. Main Findings: As a result, it becomes obvious that the boundaries of the artistic concepts are extremely blurred and much wider than the boundaries of the corresponding non-artistic concepts. In conclusion, the concept light is frequently used in English artistic discourses, i.e. significant in the culture, and a special status in the semantic space of the concept light has the meaning of knowledge and information. Applications: The study results can be used by students and universities. Novelty/Originality: In this research, a model of the semantic features of the phraseological units with the component light is presented in a comprehensive and complete manner

Utilization of an In Vivo Reporter for High Throughput Identification of Branched Small Molecule Regulators of Hypoxic Adaptation

SummarySmall molecules inhibiting hypoxia inducible factor (HIF) prolyl hydroxylases (PHDs) are the focus of drug development efforts directed toward the treatment of ischemia and metabolic imbalance. A cell-based reporter produced by fusing HIF-1α oxygen degradable domain (ODD) to luciferase was shown to work as a capture assay monitoring stability of the overexpressed luciferase-labeled HIF PHD substrate under conditions more physiological than in vitro test tubes. High throughput screening identified novel catechol and oxyquinoline pharmacophores with a “branching motif” immediately adjacent to a Fe-binding motif that fits selectively into the HIF PHD active site in in silico models. In accord with their structure-activity relationship in the primary screen, the best “hits” stabilize HIF1α, upregulate known HIF target genes in a human neuronal line, and exert neuroprotective effects in established model of oxidative stress in cortical neurons