Association of SSR markers with contents of fatty acids in olive oil and genetic diversity analysis of an olive core collection

The purpose of this research was to characterize an olive core collection using some agronomic characters and simple sequence repeat (SSR) markers and to determine SSR markers associated with the content of fatty acids in olive oil. SSR marker analysis demonstrated the presence of a high amount of genetic variation between the olive cultivars analyzed. A UPGMA dendrogram demonstrated that olive cultivars did not cluster on the basis of their geographic origin. Fatty acid components of olive oil in these cultivars were determined. The results also showed that there was a great amount of variation between the olive cultivars in terms of fatty acid composition. For example, oleic acid content ranged from 57.76 to 76.9% with standard deviation of 5.10%. Significant correlations between fatty acids of olive oil were observed. For instance, a very high negative correlation (-0.812) between oleic and linoleic acids was detected. A structured association analysis between the content of fatty acids in olive oil and SSR markers was performed. STRUCTURE analysis assigned olive cultivars to two gene pools (K = 2). Assignment of olive cultivars to these gene pools was not based on geographical origin. Association between fatty acid traits and SSR markers was evaluated using the general linear model of TASSEL. Significant associations were determined between five SSR markers and stearic, oleic, linoleic, and linolenic acids of olive oil. Very high associations (P < 0.001) between ssrOeUA-DCA14 and stearic acid and between GAPU71B and oleic acid indicated that these markers could be used for marker-assisted selection in olive

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

The purpose of this study was to characterize olive core collection with amplified fragment length polymorphism (AFLP) markers and fruit traits and to determine AFLP markers significantly associated with these fruit characters in olive. A total of 168 polymorphic AFLP markers generated by five primer combinations and nine fruit traits were used to characterize relationships between 18 olive cultivars. Although all olive cultivars were discriminated from each other by either AFLP markers (<0.75 similarity level) or fruit traits, clustering based on the AFLP markers and fruit traits was not significantly correlated (r = 0.13). Partial clustering of olive cultivars by AFLP markers according to their geographical origin was observed. Associations of AFLP markers with fruits were determined using a multiple-regression analysis with stepwise addition of AFLP markers. Significant associations between eight AFLP markers and fruit traits were identified. While five AFLP markers demonstrated significant negative correlation with fruit and stone weight, width and length and total polyphenols (P < 0.05), three AFLP markers displayed significant positive correlation with a-tocopherol and.-tocopherol (P < 0.01). This is the first report on the association of molecular markers with fruit traits in olive. Molecular markers associated with morphological and agronomic traits could be utilized for the breeding of olive cultivars. However, the association power of these markers needs to be confirmed in larger populations, and highly correlated markers should then be converted to PCR-based DNA markers such as sequence-characterized amplified region markers for better utilization

A Hybrid Type I + III Inverse Seesaw Mechanism in U(1)R−LU(1)_{R-L}-symmetric MSSM

We show that, in a $U(1)_{R-L}$-symmetric supersymmetric model, the pseudo-Dirac bino and wino can give rise to three light neutrino masses through effective operators, generated at the messenger scale between a SUSY breaking hidden sector and the visible sector. The neutrino-bino/wino mixing follows a hybrid type I+III inverse seesaw pattern. The light neutrino masses are governed by the ratio of the $U(1)_{R-L}$-breaking gravitino mass, $m_{3/2}$, and the messenger scale $\Lambda_M$. The charged component of the $SU(2)_L$-triplet, here the lightest charginos, mix with the charged leptons and generate flavor-changing neutral currents at tree level. We find that resulting lepton flavor violating observables yield a lower bound on the messenger scale, $\Lambda_M \gtrsim (500-1000)~{\rm TeV}$ for a simplified hybrid mixing scenario. We identify interesting mixing structures for certain $U(1)_{R-L}$-breaking singlino/tripletino Majorana masses. For example, in some parameter regimes, bino or wino has no mixing with the electron neutrino. We also describe the rich collider phenomenology expected in this neutrino-mass generation mechanism.Comment: v2: 20 pages, 7 figures, plots updated, added clarifying comments, matches published version in JHE

A prospective study of serum concentrations of leptin, homocysteine and insulin resistance in children with steroid-sensitive nephrotic syndrome

Aim: To measure serum leptin, homocysteine concentrations and insulin resistance in active and remission stages of children with nephrotic syndrome (NS) and to investigate their role in NS pathogenesis. Methods: A total of 70 children were included in the study, 40 patients who had been diagnosed with NS and 30 healthy patients were control. Changes in plasma concentration of the serum homocysteine, leptin, and insulin were measured and compared with the other parameters in the groups. Results: Serum leptin concentrations in active phase were lower than the remission phase (1.48 ± 0.09 ng/dl, 1.84 ± 1.64 ng/ml, p&lt;0.05). Also, serum homocysteine concentrations in NS group during the active phase were lower than the remission phase and the control group (6.45±2.54 ng/dl, 9.35±2.99 ng/ml, 7.76± 1.97 ng/ml, p&lt;0.05). The serum fasting insulin concentrations and homeostatic model assessment for insulin resistance (HOMA-IR) values of remission phase were significantly higher than those of active phase (p&lt;0.05). A positive relationship was found between the homocysteine concentrations and the body mass index of the patient; whereas, a negative relationship was detected between erythrocyte sedimentation rate (ESR), and the LDL-cholesterol concentrations (p&lt;0.05). ESR was found as the only factor associated with lower concentrations of homocysteine during the active phase (r:-0.592, p&lt;0.05).&nbsp; Conclusion: In this study, we demonstrated that serum leptin and homocysteine concentrations decreased in active phase and increased in remission phase in children with NS.&nbsp; Insulin resistance could also develop as a result of steroid use in a short period of time in these patients

Determination by Landsat Satellite Imagery to Local Scales in Land and Pollution Monitoring: A Case of Buyuk Melen Watershed (Turkey)

Buyuk Melen Watershed provides drinking water from the Western Black Sea region to Istanbul province, which Buyuk and Kucuk Melen rivers, Asar, Ugur and Aksu rivers. Many settlement areas, fertilized agricultural lands, industrial plants and solid/liquid waste dumping areas have present in Melen watershed, causing substantial pollution problems. Melen watershed has been at a serious risk of pollution that a lot of settlement areas, agricultural lands, industrial facilities, and solid and liquid waste. In this study, LANDSAT satellite data was used to monitor the status of this area on the potential of the region studied. In the watershed change of 1987, 2001, 2006 and 2010 and also supported by satellite data. However, contaminants in the watershed discharges to the inner parts as shown from the satellite data have also been observed that the increase in pollution

QTL mapping for fatty acid composition in olive oil using a high-density genetic map based on SNP markers

Olive (Olea europaea L.) is an evergreen tree species that grows naturally in regions with Mediterranean climates. Its oil and fruits are commercially valuable. Olive oil contains high levels of omega-9 (oleic acid). Because the high percentage of oleic acid makes olive oil deterioration-resistant, the development of olive varieties containing high oleic acid is one of the major goals of olive breeding programs. Therefore, this study aimed to determine quantitative trait loci (QTL) affecting the fatty acid composition of olive oil. Thus, early selection of olive genotypes with a high oleic acid content can be possible. For the determination of QTLs affecting the fatty acid composition of olive oil, a high-density genetic map was developed using a segregating olive F1 population with 121 progeny and single-nucleotide polymorphism (SNP) markers based on genotyping by sequencing (GBS). The 2892.14 cM genetic map was composed of 3254 SNP markers on 23 chromosomes, with an average distance of 0.93 cM. For QTL analysis, the fatty acid composition of the segregating olive F1 population was determined using gas chromatography in two different years. A total of 31 QTLs were discovered in the first year and 29 in the second year. Common QTLs associated with fatty acid composition in both years have been found on chromosome 1, chromosome 2, and chromosome 10. For oleic acid, 11 QTLs were discovered in the first year and 12 QTLs in the second year. With these results, the QTLs linked to fatty acid synthesis in olive oil can be used as genetic resources for marker-assisted selection (MAS) in olive breeding studies

An aggressive aneurysmal bone cyst of the proximal humerus and related complications in a pediatric patient

Clinical behavior of aneurysmal bone cyst (ABC) in younger patients can be more aggressive than that in older children and adults. Angular deformity and shortening can occur due to growth plate destruction or tumor resection. A 11-year-old boy who had been operated twice in another center for an ABC located in the left proximal humerus presented to the author’s institution with complaints of pain, deformity and shortening of the left arm. Plain radiographs revealed left proximal humerus nonunion with a large defect. Reconstruction with nonvascularized fibular autograft was applied and left upper extremity was immobilized in a velpou bandage. At the third-month follow-up, graft incorporation was observed in the distal part; however, proximal part did not show adequate healing on radiographs. Additional immobilization in a sling for 3 months was advised to the patient and his family. However, they were lost to follow-up and readmitted to the author’s institution at the 12th month postoperatively. Radiographs showed failure of the fibular graft fixation and nonunion of the humerus. Autogenic bone grafts, either vascularized or nonvascularized are the best treatment method for the large defects after tumor curettage or resection. Nonvascularized grafts are technically much easier to use than vascularized grafts and provide excellent structural bone support at the recipient side. However, they may take several months to be fully incorporated. In addition, good therapeutic outcomes require patience and collaboration with the patient and parents. Most importantly, the patient should be monitored closely

Comparison of intraperitoneal honey and sodium hyaluronate-carboxymethylcellulose (SeprafilmTM) for the prevention of postoperative intra-abdominal adhesions

BACKGROUND: Abdominal surgery can lead to postoperative intra-abdominal adhesions (PIAAs) with significant morbidity and mortality. This study compares the use of honey with a standard bioresorbable membrane (Seprafilm tm) to prevent the formation of PIAAs in rats. METHODS: Thirty rats underwent laparotomy, and PIAAs were induced by scraping the cecum. The animals were divided into three groups, each containing ten rats. Group 1 (control) represented the cecal abrasion group, with no intraperitoneal administration of any substance. Group 2 (honey group) underwent cecal abrasion and intraperitoneal administration of honey. Group 3 (Seprafilm tm group) underwent cecal abrasion and intraperitoneal Seprafilm tm application. RESULTS: Group 1 exhibited higher adhesion scores for adhesions between the abdominal wall and the organs. Groups 2 and 3 had decreased adhesive attachments to the intra-abdominal structures. Compared to group 1, the incidence of adhesion formation was lower in both group 2 (p=0.001) and group 3 (p=0.001). The incidence of fibrosis was also lower in group 2 (p=0.016) and group 3 (p=0.063) compared to group 1. There was no significant difference between the histopathological fibrosis scores for the rats in group 2 and those in group 3 (p= 0.688). CONCLUSION: This study suggests that both honey and Seprafilm tm decrease the incidence of PIAAs in the rat cecal abrasion model. Although the mechanism of action is not clear, intraperitoneal administration of honey reduced PIAAs. The outcome of this study demonstrates that honey is as effective as Seprafilm tm in preventing PIAAs

Comparison of Intraperitoneal Honey and Sodium Hyaluronate-Carboxymethylcellulose (Seprafilm™) for the Prevention of Postoperative Intra-Abdominal Adhesions

BACKGROUND: Abdominal surgery can lead to postoperative intra-abdominal adhesions (PIAAs) with significant morbidity and mortality. This study compares the use of honey with a standard bioresorbable membrane (Seprafilm tm) to prevent the formation of PIAAs in rats. METHODS: Thirty rats underwent laparotomy, and PIAAs were induced by scraping the cecum. The animals were divided into three groups, each containing ten rats. Group 1 (control) represented the cecal abrasion group, with no intraperitoneal administration of any substance. Group 2 (honey group) underwent cecal abrasion and intraperitoneal administration of honey. Group 3 (Seprafilm tm group) underwent cecal abrasion and intraperitoneal Seprafilm tm application. RESULTS: Group 1 exhibited higher adhesion scores for adhesions between the abdominal wall and the organs. Groups 2 and 3 had decreased adhesive attachments to the intra-abdominal structures. Compared to group 1, the incidence of adhesion formation was lower in both group 2 (p=0.001) and group 3 (p=0.001). The incidence of fibrosis was also lower in group 2 (p=0.016) and group 3 (p=0.063) compared to group 1. There was no significant difference between the histopathological fibrosis scores for the rats in group 2 and those in group 3 (p= 0.688). CONCLUSION: This study suggests that both honey and Seprafilm tm decrease the incidence of PIAAs in the rat cecal abrasion model. Although the mechanism of action is not clear, intraperitoneal administration of honey reduced PIAAs. The outcome of this study demonstrates that honey is as effective as Seprafilm tm in preventing PIAAs