Anaplasma phagocytophilum in ticks and tissues collected from wild birds in Romania

Abstract. Anaplasma phagocytophilum are potentially emerging tick-borne pathogen, whereas many issues about ecology, reservoir host specificity, are still unclear. The material analyzed in this study was collected along 5 years (2009)(2010)(2011)(2012)(2013)(2014)(2015) of fieldwork from 88 locations, from 32 out of 42 counties of Romania. A total of 3,794 birds belonging to 125 species were assessed, made up by 879 carcasses and 2,915 alive birds. A total of 278 birds belonging to 37 species were found infested with ticks (9.53%), with individual prevalence ranging from 0 to 50%. Anaplasma spp. were detected in 8 cases (1.7%) of 459 analyzed ticks collected from two specimens of Rook one Robin, one Blackbird and one Chaffinch. The ticks found to carry Anaplasma spp., were Haemaphysalis concinna (1 larvae), I. arboricola (4 larvae), and I. ricinus (2 larvae and 2 nymphs). Tissue samples resulted in the detection of Anaplasma spp. from heart of one Robin and one Song Thrush, with a relative prevalence of 1.66%. The low prevalence of A. phagocytophilum in bird-fed ticks corresponds to previous investigations, suggesting that birds have a reduced reservoir competence for human granulocytic anaplasmosis agents