ARTIST???S CREATIVE CONTRIBUTIONS IN THE CONTEXT OF INTERDISCIPLINARY RESEARCH

While expectations regarding art???s potential contributions to the interdisciplinary research context continue to grow, the creative endeavors of individual artists remain under-examined, perhaps because of the inter-relational nature of joint research settings. To explore, how artists navigate their contribution to a given research community, this study reviews the art practice of Seung-Hyun Ko, who participated in Science Walden, a Convergent Research Center carrying out an interdis-ciplinary research project that aimed to build an ecologically sustainable community. Drawing on comprehensive views of creativity that emphasize the importance of the social context in which the efforts of individuals emerge and are assessed, the study examines Ko???s recent collaborative practice in Science Walden within the larger context of his long-term practice as a leading artist of Yatoo, a bioregionally conscious artist community. Ko???s responses to the opportunities and challenges of his involvement in these two interrelated contexts disclose the value of the creative dynamics of interdisciplinary research, with implications for the increasingly diverse interdisciplinary research practices emerging within science and technology

Using Genomic Sequencing for Classical Genetics in E. coli K12

We here develop computational methods to facilitate use of 454 whole genome shotgun sequencing to identify mutations in Escherichia coli K12. We had Roche sequence eight related strains derived as spontaneous mutants in a background without a whole genome sequence. They provided difference tables based on assembling each genome to reference strain E. coli MG1655 (NC_000913). Due to the evolutionary distance to MG1655, these contained a large number of both false negatives and positives. By manual analysis of the dataset, we detected all the known mutations (24 at nine locations) and identified and genetically confirmed new mutations necessary and sufficient for the phenotypes we had selected in four strains. We then had Roche assemble contigs de novo, which we further assembled to full-length pseudomolecules based on synteny with MG1655. This hybrid method facilitated detection of insertion mutations and allowed annotation from MG1655. After removing one genome with less than the optimal 20- to 30-fold sequence coverage, we identified 544 putative polymorphisms that included all of the known and selected mutations apart from insertions. Finally, we detected seven new mutations in a total of only 41 candidates by comparing single genomes to composite data for the remaining six and using a ranking system to penalize homopolymer sequencing and misassembly errors. An additional benefit of the analysis is a table of differences between MG1655 and a physiologically robust E. coli wild-type strain NCM3722. Both projects were greatly facilitated by use of comparative genomics tools in the CoGe software package (http://genomevolution.org/)

Slow violence and toxic geographies : ‘out of sight’ to whom?

Toxic pollution is a form of violence. This article explores the gradual brutalities that communities surrounded by petrochemical infrastructure endure over time. Contributing to political geographies of violence and environmental justice, this paper puts the concept of ‘slow violence’ into critical comparison with work on ‘structural violence’. In doing so, the paper makes two key contributions: First, it emphasizes the intimate connections between structural and slow forms of harm, arguing that structural inequality can mutate into noxious instances of slow violence. Second, the paper pushes back against framings of toxic landscapes as entirely invisible to the people they impact. Instead of accepting the standard definition of slow violence as ‘out of sight’, we have to instead ask the question: ‘out of sight to whom?’ In asking this question, and taking seriously the knowledge claims of communities who inhabit toxic spaces, we can begin to unravel the political structures that sustain the uneven geographies of pollution. Based on long-term ethnographic research in a postcolonial region of Louisiana, nicknamed ‘Cancer Alley’, this paper reveals how people gradually ‘witness’ the impacts of slow violence in their everyday lives. Finally, drawing on the notion of ‘epistemic violence’, the paper suggests that slow violence does not persist due to a lack of arresting stories about pollution, but because these stories do not count, thus rendering certain populations and geographies vulnerable to sacrifice

Back the bid: the 2012 summer games and the governance of London

The Olympic Park being developed in east London for the 2012 Games is one large urban renewal project among many in the city. The impact of the Games on urban development may be of less significance than the impact on city politics. Bidding for and delivering the Games has contributed to a reassessment of the recent experiment with mayoral government. The paper examines these changing representations of the structures of London government that are now seen as a success. Much of the literature on Olympic cities is highly critical of the impact of the games, but the (current) substantial support for London2012 also needs to be explained. We examine how London has created opportunities for support, and moments and spaces for celebration when political leaders and Londoners can come together around particular representations of themselves and the city

Genetic diversity in invasive populations of Argentine stem weevil associated with adaptation to biocontrol

Modified, agricultural landscapes are susceptible to damage by insect pests. Biological control of pests is typically successful once a control agent has established, but this depends on the agent’s capacity to co-evolve with the host. Theoretical studies have shown that different levels of genetic variation between the host and the control agent will lead to rapid evolution of resistance in the host. Although this has been reported in one instance, the underlying genetics have not been studied. To address this, we measured the genetic variation in New Zealand populations of the pasture pest, Argentine stem weevil (Listronotus bonariensis), which is controlled with declining effectiveness by a parasitoid wasp, Microctonus hyperodae. We constructed a draft reference genome of the weevil, collected samples from a geographical survey of 10 sites around New Zealand, and genotyped them using a modified genotyping-by-sequencing approach. New Zealand populations of Argentine stem weevil have high levels of heterozygosity and low population structure, consistent with a large effective population size and frequent gene flow. This implies that Argentine stem weevils were able to evolve more rapidly than their biocontrol agent, which reproduces asexually. These findings show that monitoring genetic diversity in biocontrol agents and their targets is critical for long-term success of biological control

The \u3cem\u3eChlamydomonas\u3c/em\u3e Genome Reveals the Evolution of Key Animal and Plant Functions

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella

Sedimentary Environment Influences the Effect of an Infaunal Suspension Feeding Bivalve on Estuarine Ecosystem Function

The suspension feeding bivalve Austrovenus stutchburyi is a key species on intertidal sandflats in New Zealand, affecting the appearance and functioning of these systems, but is susceptible to several environmental stressors including sedimentation. Previous studies into the effect of this species on ecosystem function have been restricted in space and time, limiting our ability to infer the effect of habitat change on functioning. We examined the effect of Austrovenus on benthic primary production and nutrient dynamics at two sites, one sandy, the other composed of muddy-sand to determine whether sedimentary environment alters this key species' role. At each site we established large (16 m2) plots of two types, Austrovenus addition and removal. In winter and summer we deployed light and dark benthic chambers to quantify oxygen and nutrient fluxes and measured sediment denitrification enzyme activity to assess denitrification potential. Rates of gross primary production (GPP) and ammonium uptake were significantly increased when Austrovenus was added, relative to removed, at the sandy site (GPP, 1.5 times greater in winter and summer; ammonium uptake, 8 times greater in summer; 3-factor analysis of variance (ANOVA), p<0.05). Denitrification potential was also elevated in Austrovenus addition plots at the sandy site in summer (by 1.6 times, p<0.1). In contrast, there was no effect of Austrovenus treatment on any of these variables at the muddy-sand site, and overall rates tended to be lower at the muddy-sand site, relative to the sandy site (e.g. GPP was 2.1 to 3.4 times lower in winter and summer, respectively, p<0.001). Our results suggest that the positive effects of Austrovenus on system productivity and denitrification potential is limited at a muddy-sand site compared to a sandy site, and reveal the importance of considering sedimentary environment when examining the effect of key species on ecosystem function

Do you get us? A multi-experiment, meta-analytic test of the effect of felt understanding in intergroup relations

This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record Data and materials for all studies are available on the project OSF page: https://osf.io/3ye72/?view_only=13a1c3aef3044bc78d94b6ca9c8cf05a.Felt understanding is a key determinant of positive inter-personal relations, but its role and potential benefits in intergroup relations have been neglected. In the first multi-study, pre-registered test of its intergroup effects, we manipulated intergroup felt understanding (understood vs. misunderstood by an outgroup) in six studies (N = 1195) and meta-analyzed its effects. The results in most intergroup contexts supported the prediction that feeling understood (vs. misunderstood) would lead to more positive intergroup orientations (r = 0.25) and action intentions (r = 0.12). These effects were distinct from the effects of feeling liked by an outgroup, which was also manipulated in each study. An important caveat was that the felt understanding manipulation's effect reversed when the outgroup was stereotypically low in competence, but high in warmth (older adults). Overall, the findings indicate the value of incorporating felt understanding into models of intergroup relations and how those relations can be improved

Uncommon worlds: toward an ecological aesthetics of childhood in the Anthropocene

In addressing the need for a more robust engagement with aesthetics in posthumanist studies of childhood and nature, this chapter makes some tentative steps towards an ecological aesthetics of childhood that is responsive to Whitehead’s speculative philosophy. In doing so, the chapter takes an alternative theoretical approach from much of the ‘common worlds’ scholarship that has emerged in recent years, while making the case for a new aesthetics of childhood that is responsive to the accelerating social, technological, and environmental changes of the Anthropocene epoch. Our approach foregrounds the singularity of children’s aesthetic experiences as relational-qualitative ‘intensities’ that alter the fabric of nature as an extensive continuum held in common. We therefore argue that every moment in the life of a child is an uncommon and unrepeatable occasion through which the common world of nature is felt, perceived, and experienced differently. This eco-aesthetic approach is developed further through the analysis of photographs taken by children as part of the Climate Change and Me project, which has mapped children and young people’s affective responses to climate change over a period of three years in New South Wales, Australia. Rather than working with images as representations or analogic signifiers for children’s experience, we analyse how each photograph co-implicates children’s bodies and environments through affective vectors of feeling, or ‘prehensions’. This leads us to reframe aesthetic notions of image, sensibility, perception, and causality in relational terms, while also acknowledging the individuation of childhood experiences as ‘creaturely becomings’ that produce new potentials for environmental thought and behaviour