Clathrin and AP2 are required for PtdIns(4,5)P2-mediated formation of LRP6 signalosomes

Canonical Wnt signaling is initiated by the binding of Wnt proteins to their receptors, low-density lipoprotein-related protein 5 and 6 (LRP5/6) and frizzled proteins, leading to phosphatidylinositol (4,5)bisphosphate (PtdIns(4,5)P2) production, signalosome formation, and LRP phosphorylation. However, the mechanism by which PtdIns(4,5)P2 regulates the signalosome formation remains unclear. Here we show that clathrin and adaptor protein 2 (AP2) were part of the LRP6 signalosomes. The presence of clathrin and AP2 in the LRP6 signalosomes depended on PtdIns(4,5)P2, and both clathrin and AP2 were required for the formation of LRP6 signalosomes. In addition, WNT3A-induced LRP6 signalosomes were primarily localized at cell surfaces, and WNT3A did not induce marked LRP6 internalization. However, rapid PtdIns(4,5)P2 hydrolysis induced artificially after WNT3A stimulation could lead to marked LRP6 internalization. Moreover, we observed WNT3A-induced LRP6 and clathrin clustering at cell surfaces using super-resolution fluorescence microscopy. Therefore, we conclude that PtdIns(4,5)P2 promotes the assembly of LRP6 signalosomes via the recruitment of AP2 and clathrin and that LRP6 internalization may not be a prerequisite for Wnt signaling to β-catenin stabilization

Vortex-glass phase transition and superconductivity in an under- doped (Ba,K)Fe2As2 single crystal

Measurements of magnetotransport and current-voltage (I-V) characteristics up to 9 T were used to investigate the vortex phase diagram of an under-doped Measurements of magnetotransport and current-voltage (I-V) characteristics up to 9 T were used to investigate the vortex phase diagram of an under-doped (Ba,K)Fe2As2 single crystal with Tc=26.2 K. It is found that the anisotropy ratio of the upper critical field Hc2 decreases from 4 to 2.8 with decreasing temperature from Tc to 24.8 K. Consistent with the vortex-glass theory, the I-V curves measured at H=9 T can be well scaled with the vortex-glass transition temperature of Tg=20.7 K and critical exponents z=4.1 and v=1. Analyses in different magnetic fields produced almost identical critical exponent values, with some variation in Tg, corroborating the existence of the vortex-glass transition in this under-doped (Ba,K)Fe2As2 single crystal up to 9 T. A vortex phase diagram is presented, based on the evolution of Tg and Hc2 with magnetic field.Comment: 23 pages, 7 figures. accepted in Phys. Rev.

Low-magnetic-field control of dielectric constant at room temperature realized in Ba0.5Sr1.5Zn2Fe12O22

We show that room temperature resistivity of Ba0.5Sr1.5Zn2Fe12O22 single crystals increases by more than three orders of magnitude upon being subjected to optimized heat treatments. The increase in the resistivity allows the determination of magnetic field (H)-induced ferroelectric phase boundaries up to 310 K through the measurements of dielectric constant at a frequency of 10 MHz. Between 280 and 310 K, the dielectric constant curve shows a peak centered at zero magnetic field and thereafter decreases monotonically up to 0.1 T, exhibiting a magnetodielectric effect of 1.1%. This effect is ascribed to the realization of magnetic field-induced ferroelectricity at an H value of less than 0.1 T near room temperature. Comparison between electric and magnetic phase diagrams in wide temperature- and field-windows suggests that the magnetic field for inducing ferroelectricity has decreased near its helical spin ordering temperature around 315 K due to the reduction of spin anisotropy in Ba0.5Sr1.5Zn2Fe12O22

Electric field control of nonvolatile four-state magnetization at room temperature

We find the realization of large converse magnetoelectric (ME) effects at room temperature in a multiferroic hexaferrite Ba$_{0.52}$Sr$_{2.48}$Co$_{2}$Fe$_{24}$O$_{41}$ single crystal, in which rapid change of electric polarization in low magnetic fields (about 5 mT) is coined to a large ME susceptibility of 3200 ps/m. The modulation of magnetization then reaches up to 0.62 $\mu$$_{B}$/f.u. in an electric field of 1.14 MV/m. We find further that four ME states induced by different ME poling exhibit unique, nonvolatile magnetization versus electric field curves, which can be approximately described by an effective free energy with a distinct set of ME coefficients

Realization of giant magnetoelectricity in helimagnets

We show that low field magnetoelectric (ME) properties of helimagnets Ba0.5Sr1.5Zn2(Fe1-xAlx)12O22 can be efficiently tailored by Al-substitution level. As x increases, the critical magnetic field for switching electric polarization is systematically reduced from ~1 T down to ~1 mT, and the ME susceptibility is greatly enhanced to reach a giant value of 2.0 x 10^4 ps/m at an optimum x = 0.08. We find that control of nontrivial orbital moment in the octahedral Fe sites through the Al-substitution is crucial for fine tuning of magnetic anisotropy and obtaining the conspicuously improved ME characteristics

Electric polarization enhancement in multiferroic CoCr2O4 crystals with Cr-site mixing

Single crystals of multiferroic cobalt chromite Co (Cr2-x Cox) O4 have been grown via several methods to have different Co3+ doping levels (x=0.0, 0.14, and 0.18). Under magnetic fields, all the crystals display electric polarization reversal below their spiral spin ordering temperatures. We find that both saturated electric polarization and magnetization under magnetic fields increase significantly with the increase in x. This result can be qualitatively explained by a broken balance between at least two electric polarization contributions existing in CoCr2 O4 and is expected to be useful in tailoring electric polarization in similar kinds of multiferroics. © 2009 American Institute of Physics.open222

Reference Dependent Preference and Fan Responses: A Text Analysis of YouTube Comments on K-League Highlights

PURPOSE This study examined whether K-league fans’ responses to game outcomes align with reference-dependent preference and loss aversion principles. METHODS We collected user comments from the 2023 K-league game highlights videos on YouTube. We identified each user’s supporting team and excluded neutral fans’ comments. Sentiment analysis using KoBERT was applied, and estimated sentiment scores served as dependent variables. We performed panel regression to test whether unexpected wins and losses generate positive and negative comments. RESULTS First, an unexpected win generates more positive comments, and an unexpected loss generates more negative comments; a reference-dependent preference exists. Second, the difference between the coefficient on upset win and the absolute value of the coefficient on upset loss is not statistically different; loss aversion does not exist. CONCLUSIONS The findings derive a deeper understanding of sports viewership and explain the difference between domestic and overseas sports fans. Also, the findings provide insights into the domestic professional sports business

Simultaneous capturing of mixed contaminants from wastewater using novel one-pot chitosan functionalized with EDTA and graphene oxide adsorbent

The emergence of inorganic and organic contaminants has raised great concerns owing to their adverse impact on human health and ecological security. Herein, first time one-pot process was applied for chitosan (CS) functionalization using graphene oxide (GO) and ethylenediaminetetraacetic acid (EDTA) for simultaneous capturing of toxic inorganic (lead (Pb2+) and cadmium (Cd2+)) and organic (ciprofloxacin (CIP) and sildenafil (SDF)) contaminants from wastewater. In this approach, we believe that CS would work as a backbone, GO would capture both inorganic and organic contaminants via electrostatic interactions, while EDTA would make complexation with heavy metals. Various parameters including pH, reaction time, concentration, reusability etc. were evaluated to achieve the best experimental result in monocomponent system. The prepared adsorbent displayed an excellent monolayer adsorption capacity of 351.20 and 264.10 mg g−1 for Pb2+ and Cd2+, respectively, while a heterogeneous sorption capacity of 75.40 and 40.90 mg g−1 for CIP and SDF, respectively. The kinetics data fitted well to Pseudo-second order (PSO) kinetics model for both types of contaminants and gave faster interaction towards metal ions (higher k2) than organic contaminants. Experimental results showed excellent adsorption efficiencies at environmental levels in the capturing of both inorganic and organic contaminants at the same time from polluted water. The capturing mechanism of both types of contaminants was explained by elemental mapping, EDS, and FT−IR spectra. Overall, easy synthesis, excellent capturing capacity, and reusability imply that the prepared adsorbent has a sufficient potential for the treatment of co-existing toxic contaminants in water