11 research outputs found
Time resolved spectroscopy of the blue fluorescence of spinach leaves
International audienc
Analysis of time-resolved chlorophyll fluorescence and backscattered signal from vegetation
International audienc
Estimation of the chlorophyll fluorescence lifetime of plant canopies : validation of a deconvolution method based on the use of a 3-D canopy mockup
International audienc
Measurement of laser-induced fluorescence decay and reflectance of plant canopies
International audienc
Remote sensing of plant canopies by active laser measurements of time resolved chlorophyll fluorescence
National audienc
Epigallocatechin gallate (EGCG) inhibits adhesion and migration of neural progenitor cells in vitro
Food supplements based on herbal products are widely used during pregnancy as part of a self-care approach. The idea that such supplements are safe and healthy is deeply seated in the general population, although they do not underlie the same strict safety regulations than medical drugs. We aimed to characterize the neurodevelopmental effects of the green tea catechin epigallocatechin gallate (EGCG), which is now commercialized as high-dose food supplement. We used the "Neurosphere Assay" to study the effects and unravel underlying molecular mechanisms of EGCG treatment on human and rat neural progenitor cells (NPCs) development in vitro. EGCG alters human and rat NPC development in vitro. It disturbs migration distance, migration pattern, and nuclear density of NPCs growing as neurospheres. These functional impairments are initiated by EGCG binding to the extracellular matrix glycoprotein laminin, preventing its binding to β1-integrin subunits, thereby prohibiting cell adhesion and resulting in altered glia alignment and decreased number of migrating young neurons. Our data raise a concern on the intake of high-dose EGCG food supplements during pregnancy and highlight the need of an in vivo characterization of the effects of high-dose EGCG exposure during neurodevelopment