Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells

Transcription factors, chromatin components and chromatin modification activities are involved in many diseases including cancer. However, the means by which alterations in these factors influence the epigenotype of specific cell types is poorly understood. One problem that limits progress is that regulatory regions of eukaryotic genes sometimes extend over large regions of DNA. To improve chromatin structure–function analysis over such large regions, we have developed an automated, relatively simple procedure that uses magnetic beads and a capillary sequencer for ligation-mediated-PCR (LM-PCR). We show that the procedure can be used for the rapid examination of chromatin fine-structure, nucleosome positioning as well as changes in transcription factor binding-site occupancy during cellular differentiation

Valuing Urban Wetlands of the Gnangara Mound: A Hedonic Property Price Approach in Western Australia

Up to 60% of potable water supplied to Perth in Western Australia is extracted from the Gnangara mound. Many of the urban wetlands above the Mound are groundwater-dependent. Excessive groundwater extraction and climate change have resulted in a decline in water levels in the wetlands. This study estimates the value of urban wetlands in three local government districts in the Perth metropolitan region using the hedonic property price approach. Preliminary results found that proximity to wetlands influences the sales prices of properties. The marginal implicit price of reducing the distance to the nearest wetland by 1 metre, evaluated at the mean sales value, is AU$463. If there is more than one wetland within 1.5 kilometres of a property, the second wetland will help increase the property price by AU$6,081. For a 50 ha wetland, we estimate the total premium of on sales due to wetland proximity is AU$220 million, based on average property characteristics and medium house density. These results will help inform policy makers and land developers on the value of conserving existing urban wetlands, creating new wetland areas and urbanising rural wetlands.groundwater, housing development, aquifer, marginal implicit price, Land Economics/Use,

The welfare costs of urban outdoor water restrictions

Outdoor water restrictions are usually implemented as bans on a particular type of watering technology (sprinklers), which allow households to substitute for labour-intensive (hand-held) watering. This paper presents a household production model approach to analysing the impact of sprinkler restrictions on consumer welfare and their efficacy as a demand management tool. Central to our empirical analysis is an experimentally derived production function which describes the relationship between irrigation and lawn quality. We demonstrate that for a typical consumer complete sprinkler bans may be little more effective than milder restrictions policies, but are substantially more costly to the household.household model, urban water demand, urban water restrictions, Resource /Energy Economics and Policy,

Developing an off-farm agricultural education program, Oliver Springs, Tennessee

Purpose The purpose of the study was to ascertain what skills and attitudes are needed for employment in off-farm occupations in the Oliver Springs High School area. Method A survey form was developed by the investigator and information was secured by personal interview from agricultural businesses in the Oliver Springs High School area. Summary 1. Off-farm agricultural cooperative training stations exist in the Oliver Springs school area. 2. The participating employers felt a degree of competency was needed in thirteen general business skills, seventeen agri-cultural mechanical skills, thirteen animal science skills and six teen crop and soil science skills. Twenty-six additional skills were listed as needing special emphasis for employment in agricultural businesses. 3. The employers felt that a development of personal traits within prospective workers was important and they, along with the school and individual, should share in the task of instilling it within prospective workers. 4. The employers were willing to help the vocational agri-culture department plan and carry out an off-farm program. 5. The employers felt that vocational agriculture depart-ments have the major role of developing a knowledge of technical agriculture in prospective off-farm employees. 6. It was indicated that personality development in off-farm employment was more important than academic and technical skills

Crafting Courts in New Democracies: The Politics of Subnational Judicial Reform in Brazil and Mexico

Why does the strength of local courts vary in new democracies? Highlighting empirical and theoretical puzzles generated by the state-level variation in court strength within Latin Americas two largest democracies, Brazil and Mexico, this study offers a historical institutional explanation of judicial change. Notably, in contrast to much \u27new institutionalist\u27 work — which examines the effects of formal instititutional arrangements — judicial institutions here are the dependent variable. The theoretical framework builds on existing explanations regarding the effects of electoral competition and ideology, specifying underlying causal logics and mechanisms. The framework also highlights the role of actors internal to institutions (judges), and the importance of social movement theory for understanding interactions between ideological judges and sympathetic actors outside the institution, leading to judicial mobilization or behavior \u27beyond the bench. The empirical analysis draws on the analytic leverage of a subnational level of analysis and integrates quantitative and qualitative methods, yielding conclusions that would be impossible using either method in isolation. First, time-series cross-section analyses of judicial spending (as a proxy for court strength) examine broad relationships across Brazil\u27s 26 states from 1985 to 2006 and Mexico\u27s 31 states from 1993-2007. Quantitative tools for case selection identify \u27nested\u27, model-testing cases, around which I build small-N research designs consisting of three states in each country. The in-depth, qualitative analysis draws on 115 personal, semi-structured interviews with judges and other legal elites, archival evidence, and direct observation to trace the process of judicial change. Overall, electoral competition operates as a pre-condition for reform, but its effect is indeterminate once a minimum threshold of competition is crossed. Ideology has the most consistent and meaningful effect on reform. Actors and their intentions matter. However, the expression of these intentions is contingent upon the nature of opportunity structures, including mobilization strategies and alliances, as well as overlapping historical processes. In short, I find that strong reforms are most likely where progressive judges coincide with sympathetic, left-of-center politicians. The results emphasize the role of ideas and the conditional expression of these ideas, that is, the contingency of intentionality

An application of the requirement vs capability analysis to estimating design reliability of solid rocket motors

Design reliability parameters for solid propellant rocket engine